Detection of "<it>Rickettsia </it>sp. strain Uilenbergi" and "<it>Rickettsia </it>sp. strain Davousti" in <it>Amblyomma tholloni </it>ticks from elephants in Africa
<p>Abstract</p> <p>Background</p> <p>To date, 6 tick-borne rickettsiae pathogenic for humans are known to occur in Africa and 4 of them were first identified in ticks before being recognized as human pathogens.</p> <p>Results</p> <p>We examined 3...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2007-08-01
|
| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/7/74 |
| _version_ | 1828418339307782144 |
|---|---|
| author | Jeffery Kathryn Rolain Jean-Marc Parola Philippe Matsumoto Kotaro Raoult Didier |
| author_facet | Jeffery Kathryn Rolain Jean-Marc Parola Philippe Matsumoto Kotaro Raoult Didier |
| author_sort | Jeffery Kathryn |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>To date, 6 tick-borne rickettsiae pathogenic for humans are known to occur in Africa and 4 of them were first identified in ticks before being recognized as human pathogens.</p> <p>Results</p> <p>We examined 33 and 5 <it>Amblyomma tholloni </it>ticks from African elephants in the Central African Republic and Gabon, respectively, by PCR amplification and sequencing of a part of <it>gltA </it>and <it>ompA </it>genes of the genus <it>Rickettsia</it>. The partial sequences of <it>gltA </it>and <it>ompA </it>genes detected in tick in Gabon had 99.1% similarity with those of <it>R. heilongjiangensis </it>and 97.1% with those of <it>Rickettsia </it>sp. HL-93 strain, respectively. The partial <it>gltA </it>and <it>ompA </it>gene sequences detected in tick in the Central African Republic were 98.9% and 95.1% similar to those of <it>Rickettsia </it>sp. DnS14 strain and <it>R. massiliae</it>, respectively. Phylogenetic analysis showed <it>Rickettsia </it>sp. detected in Gabon clusters with <it>R. japonica </it>and <it>R. heilongjiangensis </it>in a phylogenetic tree based on the partial <it>gltA </it>and <it>ompA </it>genes. The genotype of the <it>Rickettsia </it>sp. detected in the Central African Republic is close to those of <it>R. massiliae </it>group in the phylogenetic tree based on partial <it>gltA </it>gene sequences, and distantly related to other rickettsiae in the tree based on partial <it>ompA </it>gene.</p> <p>Conclusion</p> <p>The degrees of similarity of partial <it>gltA </it>and <it>ompA </it>genes with recognized species indicate the rickettsiae detected in this study may be new species although we could only study the partial sequences of 2 genes regarding the amount of DNA that was available. We propose the <it>Rickettsia </it>sp. detected in Gabon be provisionally named "<it>Rickettsia sp</it>. stain Davousti" and <it>Rickettsia </it>sp. detected in the Central African Republic be named "<it>Rickettsia sp. </it>strain Uilenbergi".</p> |
| first_indexed | 2024-12-10T14:35:10Z |
| format | Article |
| id | doaj.art-ca2d41cbe9ac44f7aa440baa540b74ae |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-10T14:35:10Z |
| publishDate | 2007-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-ca2d41cbe9ac44f7aa440baa540b74ae2022-12-22T01:44:51ZengBMCBMC Microbiology1471-21802007-08-01717410.1186/1471-2180-7-74Detection of "<it>Rickettsia </it>sp. strain Uilenbergi" and "<it>Rickettsia </it>sp. strain Davousti" in <it>Amblyomma tholloni </it>ticks from elephants in AfricaJeffery KathrynRolain Jean-MarcParola PhilippeMatsumoto KotaroRaoult Didier<p>Abstract</p> <p>Background</p> <p>To date, 6 tick-borne rickettsiae pathogenic for humans are known to occur in Africa and 4 of them were first identified in ticks before being recognized as human pathogens.</p> <p>Results</p> <p>We examined 33 and 5 <it>Amblyomma tholloni </it>ticks from African elephants in the Central African Republic and Gabon, respectively, by PCR amplification and sequencing of a part of <it>gltA </it>and <it>ompA </it>genes of the genus <it>Rickettsia</it>. The partial sequences of <it>gltA </it>and <it>ompA </it>genes detected in tick in Gabon had 99.1% similarity with those of <it>R. heilongjiangensis </it>and 97.1% with those of <it>Rickettsia </it>sp. HL-93 strain, respectively. The partial <it>gltA </it>and <it>ompA </it>gene sequences detected in tick in the Central African Republic were 98.9% and 95.1% similar to those of <it>Rickettsia </it>sp. DnS14 strain and <it>R. massiliae</it>, respectively. Phylogenetic analysis showed <it>Rickettsia </it>sp. detected in Gabon clusters with <it>R. japonica </it>and <it>R. heilongjiangensis </it>in a phylogenetic tree based on the partial <it>gltA </it>and <it>ompA </it>genes. The genotype of the <it>Rickettsia </it>sp. detected in the Central African Republic is close to those of <it>R. massiliae </it>group in the phylogenetic tree based on partial <it>gltA </it>gene sequences, and distantly related to other rickettsiae in the tree based on partial <it>ompA </it>gene.</p> <p>Conclusion</p> <p>The degrees of similarity of partial <it>gltA </it>and <it>ompA </it>genes with recognized species indicate the rickettsiae detected in this study may be new species although we could only study the partial sequences of 2 genes regarding the amount of DNA that was available. We propose the <it>Rickettsia </it>sp. detected in Gabon be provisionally named "<it>Rickettsia sp</it>. stain Davousti" and <it>Rickettsia </it>sp. detected in the Central African Republic be named "<it>Rickettsia sp. </it>strain Uilenbergi".</p>http://www.biomedcentral.com/1471-2180/7/74 |
| spellingShingle | Jeffery Kathryn Rolain Jean-Marc Parola Philippe Matsumoto Kotaro Raoult Didier Detection of "<it>Rickettsia </it>sp. strain Uilenbergi" and "<it>Rickettsia </it>sp. strain Davousti" in <it>Amblyomma tholloni </it>ticks from elephants in Africa BMC Microbiology |
| title | Detection of "<it>Rickettsia </it>sp. strain Uilenbergi" and "<it>Rickettsia </it>sp. strain Davousti" in <it>Amblyomma tholloni </it>ticks from elephants in Africa |
| title_full | Detection of "<it>Rickettsia </it>sp. strain Uilenbergi" and "<it>Rickettsia </it>sp. strain Davousti" in <it>Amblyomma tholloni </it>ticks from elephants in Africa |
| title_fullStr | Detection of "<it>Rickettsia </it>sp. strain Uilenbergi" and "<it>Rickettsia </it>sp. strain Davousti" in <it>Amblyomma tholloni </it>ticks from elephants in Africa |
| title_full_unstemmed | Detection of "<it>Rickettsia </it>sp. strain Uilenbergi" and "<it>Rickettsia </it>sp. strain Davousti" in <it>Amblyomma tholloni </it>ticks from elephants in Africa |
| title_short | Detection of "<it>Rickettsia </it>sp. strain Uilenbergi" and "<it>Rickettsia </it>sp. strain Davousti" in <it>Amblyomma tholloni </it>ticks from elephants in Africa |
| title_sort | detection of it rickettsia it sp strain uilenbergi and it rickettsia it sp strain davousti in it amblyomma tholloni it ticks from elephants in africa |
| url | http://www.biomedcentral.com/1471-2180/7/74 |
| work_keys_str_mv | AT jefferykathryn detectionofitrickettsiaitspstrainuilenbergianditrickettsiaitspstraindavoustiinitamblyommatholloniitticksfromelephantsinafrica AT rolainjeanmarc detectionofitrickettsiaitspstrainuilenbergianditrickettsiaitspstraindavoustiinitamblyommatholloniitticksfromelephantsinafrica AT parolaphilippe detectionofitrickettsiaitspstrainuilenbergianditrickettsiaitspstraindavoustiinitamblyommatholloniitticksfromelephantsinafrica AT matsumotokotaro detectionofitrickettsiaitspstrainuilenbergianditrickettsiaitspstraindavoustiinitamblyommatholloniitticksfromelephantsinafrica AT raoultdidier detectionofitrickettsiaitspstrainuilenbergianditrickettsiaitspstraindavoustiinitamblyommatholloniitticksfromelephantsinafrica |