Composition and antioxidant activity of Paeonia lactiflora petal flavonoid extract and underlying mechanisms of the protective effect on H2O2-induced oxidative damage in BRL3A cells
Flavonoids have attracted considerable attention due to their health benefits. This study aimed to investigate the flavonoid profiles and antioxidant activity of Paeonia lactiflora petal flavonoid extract (PPF). The UHPLC-ESI-Q-Exactive HF MS/MS method was established for characterization, and 21 pr...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2023-04-01
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| Series: | Horticultural Plant Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468014122000541 |
| _version_ | 1797847726029799424 |
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| author | Lei Liu Yingdan Yuan Jiajia Zuo Jun Tao |
| author_facet | Lei Liu Yingdan Yuan Jiajia Zuo Jun Tao |
| author_sort | Lei Liu |
| collection | DOAJ |
| description | Flavonoids have attracted considerable attention due to their health benefits. This study aimed to investigate the flavonoid profiles and antioxidant activity of Paeonia lactiflora petal flavonoid extract (PPF). The UHPLC-ESI-Q-Exactive HF MS/MS method was established for characterization, and 21 predominant flavonoid compounds were tentatively identified in PPF. Among them, isoscutellarein-7-(6′-acetylallosyl-(1->2)-glucoside) and scutellarin methylester were discovered in PPF for the first time. Pretreatment with PPF significantly reduced H2O2-induced cell damage, ROS accumulation, and malondialdehyde content and increased the activity of SOD, CAT, and GSH-Px in buffalo rat liver 3A (BRL3A) cells. Moreover, the expression of nuclear Factor E2-related factor (Nrf2) was upregulated by PPF, whose expression trend was consistent with that of heme oxygenase-1 (HO-1), glutamate-cysteine ligase catalytic subunit (GCLC), and NAD(P)H quinone oxidoreductase-1 (NQO1). These findings suggested that herbaceous peony flavonoids can be used as a natural bioactive agent to prevent oxidative stress. |
| first_indexed | 2024-04-09T18:16:03Z |
| format | Article |
| id | doaj.art-ca3067f1bbdb4acab2fce8a9ee49e2c2 |
| institution | Directory Open Access Journal |
| issn | 2468-0141 |
| language | English |
| last_indexed | 2024-04-09T18:16:03Z |
| publishDate | 2023-04-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Horticultural Plant Journal |
| spelling | doaj.art-ca3067f1bbdb4acab2fce8a9ee49e2c22023-04-13T04:26:57ZengKeAi Communications Co., Ltd.Horticultural Plant Journal2468-01412023-04-0192335344Composition and antioxidant activity of Paeonia lactiflora petal flavonoid extract and underlying mechanisms of the protective effect on H2O2-induced oxidative damage in BRL3A cellsLei Liu0Yingdan Yuan1Jiajia Zuo2Jun Tao3College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225009, China; College of Horticulture, Xinyang Agriculture and Forestry University, Xinyang, Henan 464000, China; College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, Jiangsu 225009, ChinaCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou, Jiangsu 225009, ChinaCollege of Horticulture and Plant Protection, Yangzhou University, Yangzhou, Jiangsu 225009, ChinaCollege of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu 225009, China; College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, Jiangsu 225009, China; Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu 225009, China; Corresponding author.Flavonoids have attracted considerable attention due to their health benefits. This study aimed to investigate the flavonoid profiles and antioxidant activity of Paeonia lactiflora petal flavonoid extract (PPF). The UHPLC-ESI-Q-Exactive HF MS/MS method was established for characterization, and 21 predominant flavonoid compounds were tentatively identified in PPF. Among them, isoscutellarein-7-(6′-acetylallosyl-(1->2)-glucoside) and scutellarin methylester were discovered in PPF for the first time. Pretreatment with PPF significantly reduced H2O2-induced cell damage, ROS accumulation, and malondialdehyde content and increased the activity of SOD, CAT, and GSH-Px in buffalo rat liver 3A (BRL3A) cells. Moreover, the expression of nuclear Factor E2-related factor (Nrf2) was upregulated by PPF, whose expression trend was consistent with that of heme oxygenase-1 (HO-1), glutamate-cysteine ligase catalytic subunit (GCLC), and NAD(P)H quinone oxidoreductase-1 (NQO1). These findings suggested that herbaceous peony flavonoids can be used as a natural bioactive agent to prevent oxidative stress.http://www.sciencedirect.com/science/article/pii/S2468014122000541Antioxidant activityFlavonoidHerbaceous peony petalOxidative stressSolid-phase extraction |
| spellingShingle | Lei Liu Yingdan Yuan Jiajia Zuo Jun Tao Composition and antioxidant activity of Paeonia lactiflora petal flavonoid extract and underlying mechanisms of the protective effect on H2O2-induced oxidative damage in BRL3A cells Horticultural Plant Journal Antioxidant activity Flavonoid Herbaceous peony petal Oxidative stress Solid-phase extraction |
| title | Composition and antioxidant activity of Paeonia lactiflora petal flavonoid extract and underlying mechanisms of the protective effect on H2O2-induced oxidative damage in BRL3A cells |
| title_full | Composition and antioxidant activity of Paeonia lactiflora petal flavonoid extract and underlying mechanisms of the protective effect on H2O2-induced oxidative damage in BRL3A cells |
| title_fullStr | Composition and antioxidant activity of Paeonia lactiflora petal flavonoid extract and underlying mechanisms of the protective effect on H2O2-induced oxidative damage in BRL3A cells |
| title_full_unstemmed | Composition and antioxidant activity of Paeonia lactiflora petal flavonoid extract and underlying mechanisms of the protective effect on H2O2-induced oxidative damage in BRL3A cells |
| title_short | Composition and antioxidant activity of Paeonia lactiflora petal flavonoid extract and underlying mechanisms of the protective effect on H2O2-induced oxidative damage in BRL3A cells |
| title_sort | composition and antioxidant activity of paeonia lactiflora petal flavonoid extract and underlying mechanisms of the protective effect on h2o2 induced oxidative damage in brl3a cells |
| topic | Antioxidant activity Flavonoid Herbaceous peony petal Oxidative stress Solid-phase extraction |
| url | http://www.sciencedirect.com/science/article/pii/S2468014122000541 |
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