Coxiella burnetii in humans and ticks in rural Senegal.
<h4>Background</h4>Q fever is a worldwide zoonotic disease caused by Coxiella burnetii. Epidemiologically, animals are considered reservoirs and humans incidental hosts.<h4>Methodology/principal findings</h4>We investigated Q fever in rural Senegal. Human samples (e.g., sera,...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2010-04-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20386603/pdf/?tool=EBI |
| _version_ | 1828087839964790784 |
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| author | Oleg Mediannikov Florence Fenollar Cristina Socolovschi Georges Diatta Hubert Bassene Jean-François Molez Cheikh Sokhna Jean-François Trape Didier Raoult |
| author_facet | Oleg Mediannikov Florence Fenollar Cristina Socolovschi Georges Diatta Hubert Bassene Jean-François Molez Cheikh Sokhna Jean-François Trape Didier Raoult |
| author_sort | Oleg Mediannikov |
| collection | DOAJ |
| description | <h4>Background</h4>Q fever is a worldwide zoonotic disease caused by Coxiella burnetii. Epidemiologically, animals are considered reservoirs and humans incidental hosts.<h4>Methodology/principal findings</h4>We investigated Q fever in rural Senegal. Human samples (e.g., sera, saliva, breast milk, feces) were screened in the generally healthy population of two villages of the Sine-Saloum region. Ticks were collected in four regions. Seroprevalence was studied by immunofluorescence, and all other samples were tested by two qPCR systems for detection of C. burnetii. Positive samples were genotyped (multispacer typing) by amplification and sequencing of three spacers. Strains were isolated by cell culture. We found that the seroprevalence may be as high as 24.5% (59 of 238 studied) in Dielmo village. We identified spontaneous excretion of C. burnetii by humans through faeces and milk. Hard and soft ticks (8 species) were infected in 0-37.6%. We identified three genotypes of C. burnetii. The previously identified genotype 6 was the most common in ticks in all studied regions and the only one found in human samples. Three strains of genotype 6 of C. burnetii were also recovered from soft tick Ornithodoros sonrai. Two other genotypes found in ticks, 35 and 36, were identified for the first time.<h4>Conclusions/significance</h4>Q fever should be considered a significant public health threat in Senegal. Humans, similar to other mammals, may continuously excrete C. burnetii. |
| first_indexed | 2024-04-11T05:17:46Z |
| format | Article |
| id | doaj.art-ca30843d136849cb9446395532fa0cb0 |
| institution | Directory Open Access Journal |
| issn | 1935-2727 1935-2735 |
| language | English |
| last_indexed | 2024-04-11T05:17:46Z |
| publishDate | 2010-04-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj.art-ca30843d136849cb9446395532fa0cb02022-12-24T05:34:12ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352010-04-0144e65410.1371/journal.pntd.0000654Coxiella burnetii in humans and ticks in rural Senegal.Oleg MediannikovFlorence FenollarCristina SocolovschiGeorges DiattaHubert BasseneJean-François MolezCheikh SokhnaJean-François TrapeDidier Raoult<h4>Background</h4>Q fever is a worldwide zoonotic disease caused by Coxiella burnetii. Epidemiologically, animals are considered reservoirs and humans incidental hosts.<h4>Methodology/principal findings</h4>We investigated Q fever in rural Senegal. Human samples (e.g., sera, saliva, breast milk, feces) were screened in the generally healthy population of two villages of the Sine-Saloum region. Ticks were collected in four regions. Seroprevalence was studied by immunofluorescence, and all other samples were tested by two qPCR systems for detection of C. burnetii. Positive samples were genotyped (multispacer typing) by amplification and sequencing of three spacers. Strains were isolated by cell culture. We found that the seroprevalence may be as high as 24.5% (59 of 238 studied) in Dielmo village. We identified spontaneous excretion of C. burnetii by humans through faeces and milk. Hard and soft ticks (8 species) were infected in 0-37.6%. We identified three genotypes of C. burnetii. The previously identified genotype 6 was the most common in ticks in all studied regions and the only one found in human samples. Three strains of genotype 6 of C. burnetii were also recovered from soft tick Ornithodoros sonrai. Two other genotypes found in ticks, 35 and 36, were identified for the first time.<h4>Conclusions/significance</h4>Q fever should be considered a significant public health threat in Senegal. Humans, similar to other mammals, may continuously excrete C. burnetii.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20386603/pdf/?tool=EBI |
| spellingShingle | Oleg Mediannikov Florence Fenollar Cristina Socolovschi Georges Diatta Hubert Bassene Jean-François Molez Cheikh Sokhna Jean-François Trape Didier Raoult Coxiella burnetii in humans and ticks in rural Senegal. PLoS Neglected Tropical Diseases |
| title | Coxiella burnetii in humans and ticks in rural Senegal. |
| title_full | Coxiella burnetii in humans and ticks in rural Senegal. |
| title_fullStr | Coxiella burnetii in humans and ticks in rural Senegal. |
| title_full_unstemmed | Coxiella burnetii in humans and ticks in rural Senegal. |
| title_short | Coxiella burnetii in humans and ticks in rural Senegal. |
| title_sort | coxiella burnetii in humans and ticks in rural senegal |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20386603/pdf/?tool=EBI |
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