Crystallographic Data Collection Using a Multilayer Monochromator on an Undulator Beamline at the Shanghai Synchrotron Radiation Facility
To resolve photons hungry for weak diffraction samples by the crystallographic method, a double-multilayer monochromator (DMM) was employed on an undulator beamline (BL17UM) at the Shanghai Synchrotron Radiation Facility (SSRF) to provide a focused sub-micron beam with high brightness for macromolec...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-02-01
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| Series: | Crystals |
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| Online Access: | https://www.mdpi.com/2073-4352/14/2/199 |
| _version_ | 1797298512958849024 |
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| author | Chenyu Zhang Qin Xu Weiwei Wang Miao Liang Li Yu Minjun Li Zhimin Zhu Liqing Huang Qianhui Li Feng Yu Yuzhu Wang Huan Zhou Qisheng Wang |
| author_facet | Chenyu Zhang Qin Xu Weiwei Wang Miao Liang Li Yu Minjun Li Zhimin Zhu Liqing Huang Qianhui Li Feng Yu Yuzhu Wang Huan Zhou Qisheng Wang |
| author_sort | Chenyu Zhang |
| collection | DOAJ |
| description | To resolve photons hungry for weak diffraction samples by the crystallographic method, a double-multilayer monochromator (DMM) was employed on an undulator beamline (BL17UM) at the Shanghai Synchrotron Radiation Facility (SSRF) to provide a focused sub-micron beam with high brightness for macromolecular crystallography experiments. High-quality crystallographic datasets from model protein crystal samples were collected and processed by an existing crystallographic program for structure solution and refinement. The data quality was compared with datasets from a normal silicon crystal monochromator to evaluate the bandwidth of the DMM effect on these crystallographic data. This experiment demonstrates that multilayer optics on an undulator beamline may play a valuable role in satisfying the demands of structure-related research, which requires novel methods. |
| first_indexed | 2024-03-07T22:36:54Z |
| format | Article |
| id | doaj.art-ca34589baec345d9899293d8e8a9caab |
| institution | Directory Open Access Journal |
| issn | 2073-4352 |
| language | English |
| last_indexed | 2024-03-07T22:36:54Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Crystals |
| spelling | doaj.art-ca34589baec345d9899293d8e8a9caab2024-02-23T15:13:21ZengMDPI AGCrystals2073-43522024-02-0114219910.3390/cryst14020199Crystallographic Data Collection Using a Multilayer Monochromator on an Undulator Beamline at the Shanghai Synchrotron Radiation FacilityChenyu Zhang0Qin Xu1Weiwei Wang2Miao Liang3Li Yu4Minjun Li5Zhimin Zhu6Liqing Huang7Qianhui Li8Feng Yu9Yuzhu Wang10Huan Zhou11Qisheng Wang12Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, ChinaShanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, ChinaShanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, ChinaShanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, ChinaShanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, ChinaShanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, ChinaShanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, ChinaShanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, ChinaShanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, ChinaShanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, ChinaShanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, ChinaShanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, ChinaShanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, ChinaTo resolve photons hungry for weak diffraction samples by the crystallographic method, a double-multilayer monochromator (DMM) was employed on an undulator beamline (BL17UM) at the Shanghai Synchrotron Radiation Facility (SSRF) to provide a focused sub-micron beam with high brightness for macromolecular crystallography experiments. High-quality crystallographic datasets from model protein crystal samples were collected and processed by an existing crystallographic program for structure solution and refinement. The data quality was compared with datasets from a normal silicon crystal monochromator to evaluate the bandwidth of the DMM effect on these crystallographic data. This experiment demonstrates that multilayer optics on an undulator beamline may play a valuable role in satisfying the demands of structure-related research, which requires novel methods.https://www.mdpi.com/2073-4352/14/2/199double-multilayer monochromatormacromolecular crystallographyroom temperature crystallography |
| spellingShingle | Chenyu Zhang Qin Xu Weiwei Wang Miao Liang Li Yu Minjun Li Zhimin Zhu Liqing Huang Qianhui Li Feng Yu Yuzhu Wang Huan Zhou Qisheng Wang Crystallographic Data Collection Using a Multilayer Monochromator on an Undulator Beamline at the Shanghai Synchrotron Radiation Facility Crystals double-multilayer monochromator macromolecular crystallography room temperature crystallography |
| title | Crystallographic Data Collection Using a Multilayer Monochromator on an Undulator Beamline at the Shanghai Synchrotron Radiation Facility |
| title_full | Crystallographic Data Collection Using a Multilayer Monochromator on an Undulator Beamline at the Shanghai Synchrotron Radiation Facility |
| title_fullStr | Crystallographic Data Collection Using a Multilayer Monochromator on an Undulator Beamline at the Shanghai Synchrotron Radiation Facility |
| title_full_unstemmed | Crystallographic Data Collection Using a Multilayer Monochromator on an Undulator Beamline at the Shanghai Synchrotron Radiation Facility |
| title_short | Crystallographic Data Collection Using a Multilayer Monochromator on an Undulator Beamline at the Shanghai Synchrotron Radiation Facility |
| title_sort | crystallographic data collection using a multilayer monochromator on an undulator beamline at the shanghai synchrotron radiation facility |
| topic | double-multilayer monochromator macromolecular crystallography room temperature crystallography |
| url | https://www.mdpi.com/2073-4352/14/2/199 |
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