Molecular characterization and nucleotide substitution of antibiotic resistance genes in multidrug-resistant Escherichia coli isolated from environmental swine farms
An increase of multi-drug resistant Escherichia coli in swine husbandry have been concerned worldwide. This study aimed to determine the multidrug resistance and nucleotide substitution of beta-lactam antibiotic and tetracycline resistant genes in E. coli from swine farms in Southern Thailand. A tot...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
KeAi Communications Co., Ltd.
2023-12-01
|
| Series: | Emerging Contaminants |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405665023000471 |
| _version_ | 1797615950679244800 |
|---|---|
| author | Ratchadaporn Boripun Phirabhat Saengsawang Sutsiree Intongead Ruethai Narinthorn Tuempong Wongtawan Veeranoot Nissapatorn Maria de Lourdes Pereira Watcharapong Mitsuwan |
| author_facet | Ratchadaporn Boripun Phirabhat Saengsawang Sutsiree Intongead Ruethai Narinthorn Tuempong Wongtawan Veeranoot Nissapatorn Maria de Lourdes Pereira Watcharapong Mitsuwan |
| author_sort | Ratchadaporn Boripun |
| collection | DOAJ |
| description | An increase of multi-drug resistant Escherichia coli in swine husbandry have been concerned worldwide. This study aimed to determine the multidrug resistance and nucleotide substitution of beta-lactam antibiotic and tetracycline resistant genes in E. coli from swine farms in Southern Thailand. A total of 112 isolates of E. coli was isolated from 50 pig farms, which were confirmed by identified by MALDI-TOF analysis. Seventy-three isolates (65.18%) and 39 isolates (34.82%) were isolated from the feces and waste water samples, respectively. One hundred percent resistance to beta-lactam antibiotics as well as their resistant gene blaTEM was detected in isolates. Furthermore, 81% isolates were tetracycline resistance and both tetA [68.42% (13/19) in feces samples, 72.73% (8/11) in waste water samples] and tetB [10.53% (2/19) in feces samples, 18.18% (2/11) in waste water samples] genes responsible for tetracycline resistance were observed. Furthermore, 54 isolates had multi-drug resistance that presented 11 different patterns. The nucleotide substitution of genes was detected in 3 isolates of E. coli, and may consider as the point mutation. The nucleotide at 859 bp of tetA gene of the isolate WU-WW009-01 was changed from T to A. While, the isolate WU-WW004-02 showed 2 nucleotide substitution sites at the position of 266 (from A to G) and 859 (from T to G) bp. The nucleotide at 36 bp of blaTEM gene of the isolate WU-F003-02 was replaced from G to A. Findings of this study may help to control the spread of E. coli antibiotic resistance genes in the swine farms. |
| first_indexed | 2024-03-11T07:34:15Z |
| format | Article |
| id | doaj.art-ca35ae9734624a70af83041f947e612b |
| institution | Directory Open Access Journal |
| issn | 2405-6650 |
| language | English |
| last_indexed | 2024-03-11T07:34:15Z |
| publishDate | 2023-12-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Emerging Contaminants |
| spelling | doaj.art-ca35ae9734624a70af83041f947e612b2023-11-17T05:27:26ZengKeAi Communications Co., Ltd.Emerging Contaminants2405-66502023-12-0194100249Molecular characterization and nucleotide substitution of antibiotic resistance genes in multidrug-resistant Escherichia coli isolated from environmental swine farmsRatchadaporn Boripun0Phirabhat Saengsawang1Sutsiree Intongead2Ruethai Narinthorn3Tuempong Wongtawan4Veeranoot Nissapatorn5Maria de Lourdes Pereira6Watcharapong Mitsuwan7Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat, 80160, Thailand; One Health Research Center, Walailak University, Nakhon Si Thammarat, 80160, ThailandAkkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat, 80160, Thailand; One Health Research Center, Walailak University, Nakhon Si Thammarat, 80160, ThailandAkkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat, 80160, ThailandAkkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat, 80160, ThailandAkkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat, 80160, ThailandSchool of Allied Health Sciences, Southeast Asia Water Team (SEA Water Team), World Union for Herbal Drug Discovery (WUHeDD), And Research Excellence Center for Innovation and Health Products (RECIHP), Walailak University, Nakhon Si Thammarat, ThailandCICECO-Aveiro Institute of Materials and Department of Medical Sciences, University of Aveiro, Aveiro, PortugalAkkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat, 80160, Thailand; One Health Research Center, Walailak University, Nakhon Si Thammarat, 80160, Thailand; Center of Excellence in Innovation of Essential Oil, Walailak University, Nakhon Si Thammarat, 80160, Thailand; Corresponding author. Akkhraratchakumari Veterinary College, Walailak University, Nakhon Si Thammarat, Thailand.An increase of multi-drug resistant Escherichia coli in swine husbandry have been concerned worldwide. This study aimed to determine the multidrug resistance and nucleotide substitution of beta-lactam antibiotic and tetracycline resistant genes in E. coli from swine farms in Southern Thailand. A total of 112 isolates of E. coli was isolated from 50 pig farms, which were confirmed by identified by MALDI-TOF analysis. Seventy-three isolates (65.18%) and 39 isolates (34.82%) were isolated from the feces and waste water samples, respectively. One hundred percent resistance to beta-lactam antibiotics as well as their resistant gene blaTEM was detected in isolates. Furthermore, 81% isolates were tetracycline resistance and both tetA [68.42% (13/19) in feces samples, 72.73% (8/11) in waste water samples] and tetB [10.53% (2/19) in feces samples, 18.18% (2/11) in waste water samples] genes responsible for tetracycline resistance were observed. Furthermore, 54 isolates had multi-drug resistance that presented 11 different patterns. The nucleotide substitution of genes was detected in 3 isolates of E. coli, and may consider as the point mutation. The nucleotide at 859 bp of tetA gene of the isolate WU-WW009-01 was changed from T to A. While, the isolate WU-WW004-02 showed 2 nucleotide substitution sites at the position of 266 (from A to G) and 859 (from T to G) bp. The nucleotide at 36 bp of blaTEM gene of the isolate WU-F003-02 was replaced from G to A. Findings of this study may help to control the spread of E. coli antibiotic resistance genes in the swine farms.http://www.sciencedirect.com/science/article/pii/S2405665023000471Escherichia coliSwine farmsMulti-drug resistanceBeta-lactam antibioticTetracycline |
| spellingShingle | Ratchadaporn Boripun Phirabhat Saengsawang Sutsiree Intongead Ruethai Narinthorn Tuempong Wongtawan Veeranoot Nissapatorn Maria de Lourdes Pereira Watcharapong Mitsuwan Molecular characterization and nucleotide substitution of antibiotic resistance genes in multidrug-resistant Escherichia coli isolated from environmental swine farms Emerging Contaminants Escherichia coli Swine farms Multi-drug resistance Beta-lactam antibiotic Tetracycline |
| title | Molecular characterization and nucleotide substitution of antibiotic resistance genes in multidrug-resistant Escherichia coli isolated from environmental swine farms |
| title_full | Molecular characterization and nucleotide substitution of antibiotic resistance genes in multidrug-resistant Escherichia coli isolated from environmental swine farms |
| title_fullStr | Molecular characterization and nucleotide substitution of antibiotic resistance genes in multidrug-resistant Escherichia coli isolated from environmental swine farms |
| title_full_unstemmed | Molecular characterization and nucleotide substitution of antibiotic resistance genes in multidrug-resistant Escherichia coli isolated from environmental swine farms |
| title_short | Molecular characterization and nucleotide substitution of antibiotic resistance genes in multidrug-resistant Escherichia coli isolated from environmental swine farms |
| title_sort | molecular characterization and nucleotide substitution of antibiotic resistance genes in multidrug resistant escherichia coli isolated from environmental swine farms |
| topic | Escherichia coli Swine farms Multi-drug resistance Beta-lactam antibiotic Tetracycline |
| url | http://www.sciencedirect.com/science/article/pii/S2405665023000471 |
| work_keys_str_mv | AT ratchadapornboripun molecularcharacterizationandnucleotidesubstitutionofantibioticresistancegenesinmultidrugresistantescherichiacoliisolatedfromenvironmentalswinefarms AT phirabhatsaengsawang molecularcharacterizationandnucleotidesubstitutionofantibioticresistancegenesinmultidrugresistantescherichiacoliisolatedfromenvironmentalswinefarms AT sutsireeintongead molecularcharacterizationandnucleotidesubstitutionofantibioticresistancegenesinmultidrugresistantescherichiacoliisolatedfromenvironmentalswinefarms AT ruethainarinthorn molecularcharacterizationandnucleotidesubstitutionofantibioticresistancegenesinmultidrugresistantescherichiacoliisolatedfromenvironmentalswinefarms AT tuempongwongtawan molecularcharacterizationandnucleotidesubstitutionofantibioticresistancegenesinmultidrugresistantescherichiacoliisolatedfromenvironmentalswinefarms AT veeranootnissapatorn molecularcharacterizationandnucleotidesubstitutionofantibioticresistancegenesinmultidrugresistantescherichiacoliisolatedfromenvironmentalswinefarms AT mariadelourdespereira molecularcharacterizationandnucleotidesubstitutionofantibioticresistancegenesinmultidrugresistantescherichiacoliisolatedfromenvironmentalswinefarms AT watcharapongmitsuwan molecularcharacterizationandnucleotidesubstitutionofantibioticresistancegenesinmultidrugresistantescherichiacoliisolatedfromenvironmentalswinefarms |