Fixation of free gingival grafts with cyanoacrylate glues: A histomorphometric and immunohistochemical study
Aim: The objective of this study was to evaluate the inflammatory process resulting from the use of two cyanoacrylate-based adhesives to stabilize grafts. Methodology: A total of 45 male Wistar rats were randomly divided into three groups (n = 15/group) treated with ethyl cyanoacrylate glue (TG1), o...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-12-01
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| Series: | Saudi Dental Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1013905221000547 |
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| author | Dayrine Silveira de Paula Lia Vila Real Lima Roberta Barroso Cavalcante Paulo Goberlânio de Barros Silva Ana Paula Negreiros Nunes Alves Bruno Rocha da Silva |
| author_facet | Dayrine Silveira de Paula Lia Vila Real Lima Roberta Barroso Cavalcante Paulo Goberlânio de Barros Silva Ana Paula Negreiros Nunes Alves Bruno Rocha da Silva |
| author_sort | Dayrine Silveira de Paula |
| collection | DOAJ |
| description | Aim: The objective of this study was to evaluate the inflammatory process resulting from the use of two cyanoacrylate-based adhesives to stabilize grafts. Methodology: A total of 45 male Wistar rats were randomly divided into three groups (n = 15/group) treated with ethyl cyanoacrylate glue (TG1), octyl-2-cyanoacrylate glue (TG2) or suture threads (CG). After de-epithelialization in the anterior gingival region of the mandible, the graft was removed from the donor site (hard palate), taken to the recipient site and stabilized according to the protocol of each group. After 7, 14, and 45 days, the animals were euthanized. The graft area was analysed macroscopically, histologically, histochemically (Masson trichrome), and immunohistochemically positive cell count for TGF-β, α-SMA, RANKL, OPG, FGF, and IL-10. The Kruskal-Wallis/Dunn test (SPSS 20.0, p < 0.05) was used for analysis. Results: There was no difference in the clinical parameters among the three groups, but TG1 showed the lowest mononuclear inflammatory cell count and the highest amount of total collagen. FGF immunoexpression was significantly higher for the CG group, but the TG2 showed a significant reduction in the RANKL/OPG ratio. Conclusion: TG1 had a mild inflammatory response and a higher collagen deposition than other glues, and TG2 had a reduction in the RANKL / OPG ratio. |
| first_indexed | 2024-12-14T23:25:07Z |
| format | Article |
| id | doaj.art-ca371db6377343768a37537c7c04eb9a |
| institution | Directory Open Access Journal |
| issn | 1013-9052 |
| language | English |
| last_indexed | 2024-12-14T23:25:07Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Saudi Dental Journal |
| spelling | doaj.art-ca371db6377343768a37537c7c04eb9a2022-12-21T22:43:49ZengElsevierSaudi Dental Journal1013-90522021-12-0133810631070Fixation of free gingival grafts with cyanoacrylate glues: A histomorphometric and immunohistochemical studyDayrine Silveira de Paula0Lia Vila Real Lima1Roberta Barroso Cavalcante2Paulo Goberlânio de Barros Silva3Ana Paula Negreiros Nunes Alves4Bruno Rocha da Silva5Division of Oral Pathology, Federal University of Ceará, Rua Monsenhor Furtado, S/N, Fortaleza, BrazilPostgraduate Program in Clinical Dentistry, Unichristus, R. João Adolfo Gurgel, 133 - Cocó, Fortaleza, Ceará, BrazilDepartment of Oral Pathology, School of Dentistry, University of Fortaleza - UNIFOR, Av. Washington Soares, 1321 Fortaleza, CE, BrazilDepartment of Dentistry, Unichristus, R. João Adolfo Gurgel, 133 - Cocó, Fortaleza, Ceará, Brazil; Division of Oral Pathology, Federal University of Ceará, Rua Monsenhor Furtado, S/N, Fortaleza, BrazilDivision of Oral Pathology, Federal University of Ceará, Rua Monsenhor Furtado, S/N, Fortaleza, BrazilSchool of Dentistry, University of Fortaleza - UNIFOR, Av. Washington Soares, 1321 Fortaleza, CE, Brazil; Corresponding author at: School of Dentistry, University of Fortaleza, UNIFOR, Avenida: Washington Soares 1321, Bloco O, Edson Queiroz, Fortaleza, CE, Brazil.Aim: The objective of this study was to evaluate the inflammatory process resulting from the use of two cyanoacrylate-based adhesives to stabilize grafts. Methodology: A total of 45 male Wistar rats were randomly divided into three groups (n = 15/group) treated with ethyl cyanoacrylate glue (TG1), octyl-2-cyanoacrylate glue (TG2) or suture threads (CG). After de-epithelialization in the anterior gingival region of the mandible, the graft was removed from the donor site (hard palate), taken to the recipient site and stabilized according to the protocol of each group. After 7, 14, and 45 days, the animals were euthanized. The graft area was analysed macroscopically, histologically, histochemically (Masson trichrome), and immunohistochemically positive cell count for TGF-β, α-SMA, RANKL, OPG, FGF, and IL-10. The Kruskal-Wallis/Dunn test (SPSS 20.0, p < 0.05) was used for analysis. Results: There was no difference in the clinical parameters among the three groups, but TG1 showed the lowest mononuclear inflammatory cell count and the highest amount of total collagen. FGF immunoexpression was significantly higher for the CG group, but the TG2 showed a significant reduction in the RANKL/OPG ratio. Conclusion: TG1 had a mild inflammatory response and a higher collagen deposition than other glues, and TG2 had a reduction in the RANKL / OPG ratio.http://www.sciencedirect.com/science/article/pii/S1013905221000547CyanoacrylatesGingivaTransplants |
| spellingShingle | Dayrine Silveira de Paula Lia Vila Real Lima Roberta Barroso Cavalcante Paulo Goberlânio de Barros Silva Ana Paula Negreiros Nunes Alves Bruno Rocha da Silva Fixation of free gingival grafts with cyanoacrylate glues: A histomorphometric and immunohistochemical study Saudi Dental Journal Cyanoacrylates Gingiva Transplants |
| title | Fixation of free gingival grafts with cyanoacrylate glues: A histomorphometric and immunohistochemical study |
| title_full | Fixation of free gingival grafts with cyanoacrylate glues: A histomorphometric and immunohistochemical study |
| title_fullStr | Fixation of free gingival grafts with cyanoacrylate glues: A histomorphometric and immunohistochemical study |
| title_full_unstemmed | Fixation of free gingival grafts with cyanoacrylate glues: A histomorphometric and immunohistochemical study |
| title_short | Fixation of free gingival grafts with cyanoacrylate glues: A histomorphometric and immunohistochemical study |
| title_sort | fixation of free gingival grafts with cyanoacrylate glues a histomorphometric and immunohistochemical study |
| topic | Cyanoacrylates Gingiva Transplants |
| url | http://www.sciencedirect.com/science/article/pii/S1013905221000547 |
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