Effect of Post -Thaw Addition of Insemination Media and Prostatic Fluid on Dog Sperm Motility Parameters
Improving sperm parameters with a role in fertilization and keeping them as long as possible at optimal levels is still a current requirement for frozen-thawed semen. The aim of the study was to evaluate the effect of the addition of an artificial insemination media (M) and prostatic fluid (PF), pos...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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AcademicPres
2018-12-01
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| Series: | Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Veterinary Medicine |
| Subjects: | |
| Online Access: | http://journals.usamvcluj.ro/index.php/veterinary/article/view/13179 |
| _version_ | 1818487133574266880 |
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| author | Violeta IGNA Ramona FENEȘ Ileana BRUDIU |
| author_facet | Violeta IGNA Ramona FENEȘ Ileana BRUDIU |
| author_sort | Violeta IGNA |
| collection | DOAJ |
| description | Improving sperm parameters with a role in fertilization and keeping them as long as possible at optimal levels is still a current requirement for frozen-thawed semen. The aim of the study was to evaluate the effect of the addition of an artificial insemination media (M) and prostatic fluid (PF), post thawing, on dog sperm motility parameters. The sperm rich fraction from nine dog ejaculates was cryopreserved. After thawing, three experimental variants were carried out: 1) semen diluted 1:1 with CaniPlus Enhance–media 2) with the PF and 3) semen without any addition. Samples were incubated at 37°C and sperm motility parameters were assessed after 10, 25, 40 and 50 minutes. Samples supplemented with the insemination media recorded the highest values of total motility, progressive motility and sperm velocity throughout incubation. Addition of PF had a negative effect on the total and progressive motility values and caused a slight increase in velocity. |
| first_indexed | 2024-12-10T16:33:17Z |
| format | Article |
| id | doaj.art-ca3a2ea1a9ce4eb5a331ece25720f6e0 |
| institution | Directory Open Access Journal |
| issn | 1843-5270 1843-5378 |
| language | English |
| last_indexed | 2024-12-10T16:33:17Z |
| publishDate | 2018-12-01 |
| publisher | AcademicPres |
| record_format | Article |
| series | Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Veterinary Medicine |
| spelling | doaj.art-ca3a2ea1a9ce4eb5a331ece25720f6e02022-12-22T01:41:30ZengAcademicPresBulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Veterinary Medicine1843-52701843-53782018-12-0175223624210.15835/buasvmcn-vm:2018.004110794Effect of Post -Thaw Addition of Insemination Media and Prostatic Fluid on Dog Sperm Motility ParametersVioleta IGNA0Ramona FENEȘ1Ileana BRUDIU2Banat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” TimisoaraBanat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” TimisoaraBanat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” TimisoaraImproving sperm parameters with a role in fertilization and keeping them as long as possible at optimal levels is still a current requirement for frozen-thawed semen. The aim of the study was to evaluate the effect of the addition of an artificial insemination media (M) and prostatic fluid (PF), post thawing, on dog sperm motility parameters. The sperm rich fraction from nine dog ejaculates was cryopreserved. After thawing, three experimental variants were carried out: 1) semen diluted 1:1 with CaniPlus Enhance–media 2) with the PF and 3) semen without any addition. Samples were incubated at 37°C and sperm motility parameters were assessed after 10, 25, 40 and 50 minutes. Samples supplemented with the insemination media recorded the highest values of total motility, progressive motility and sperm velocity throughout incubation. Addition of PF had a negative effect on the total and progressive motility values and caused a slight increase in velocity.http://journals.usamvcluj.ro/index.php/veterinary/article/view/13179dogfrozen-thawed semeninsemination mediaprostatic fluidsperm motility. |
| spellingShingle | Violeta IGNA Ramona FENEȘ Ileana BRUDIU Effect of Post -Thaw Addition of Insemination Media and Prostatic Fluid on Dog Sperm Motility Parameters Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca: Veterinary Medicine dog frozen-thawed semen insemination media prostatic fluid sperm motility. |
| title | Effect of Post -Thaw Addition of Insemination Media and Prostatic Fluid on Dog Sperm Motility Parameters |
| title_full | Effect of Post -Thaw Addition of Insemination Media and Prostatic Fluid on Dog Sperm Motility Parameters |
| title_fullStr | Effect of Post -Thaw Addition of Insemination Media and Prostatic Fluid on Dog Sperm Motility Parameters |
| title_full_unstemmed | Effect of Post -Thaw Addition of Insemination Media and Prostatic Fluid on Dog Sperm Motility Parameters |
| title_short | Effect of Post -Thaw Addition of Insemination Media and Prostatic Fluid on Dog Sperm Motility Parameters |
| title_sort | effect of post thaw addition of insemination media and prostatic fluid on dog sperm motility parameters |
| topic | dog frozen-thawed semen insemination media prostatic fluid sperm motility. |
| url | http://journals.usamvcluj.ro/index.php/veterinary/article/view/13179 |
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