Optimization of Ultrasound-Assisted Extraction of Polyphenols from <i>Myrtus communis</i> L. Pericarp
Response surface methodology (RSM) was used to optimize the extraction of phenolics from pericap of <i>Myrtus communis</i> using ultrasound-assisted extraction (UAE). The results were compared with those obtained by microwave-assisted extraction (MAE) and conventional solvent extraction...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-07-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/8/7/205 |
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| author | Nadia Bouaoudia-Madi Lila Boulekbache-Makhlouf Khodir Madani Artur M.S. Silva Sofiane Dairi Sonia Oukhmanou–Bensidhoum Susana M. Cardoso |
| author_facet | Nadia Bouaoudia-Madi Lila Boulekbache-Makhlouf Khodir Madani Artur M.S. Silva Sofiane Dairi Sonia Oukhmanou–Bensidhoum Susana M. Cardoso |
| author_sort | Nadia Bouaoudia-Madi |
| collection | DOAJ |
| description | Response surface methodology (RSM) was used to optimize the extraction of phenolics from pericap of <i>Myrtus communis</i> using ultrasound-assisted extraction (UAE). The results were compared with those obtained by microwave-assisted extraction (MAE) and conventional solvent extraction (CSE) methods. The individual compounds of the optimized extract obtained by UAE were identified by ultra-high-performance liquid chromatography coupled with diode array detection and electrospray ionization mass spectrometry (UHPLC-DAD-ESI-MS<sup>n</sup>). The yield of total phenolic compounds (TPC) was affected more significantly by ethanol concentration, irradiation time, liquid solvent-to-solid ratio (<i>p</i> < 0.0001) and amplitude (<i>p</i> = 0.0421) and optimal parameters conditions set by the RSM model were 70% (<i>v</i>/<i>v</i>), 7.5 min and 30%, respectively. The experimental yield of TPC (241.66 ± 12.77 mg gallic acid equivalent/g dry weight) confirmed the predicted value (235.52 ± 9.9 mg gallic acid equivalent/g dry weight), allowing also to confirm the model validity. Under optimized conditions, UAE was more efficient than MAE and CSE in extracting antioxidants, which comprised mostly myricetin glycosides. Globally, the present work demonstrated that, compared to MAE and CSE, UAE is an efficient method for phenolic extraction from <i>M. communis</i> pericarp, enabling to reduce the working time and the solvent consumption. |
| first_indexed | 2024-03-12T11:15:06Z |
| format | Article |
| id | doaj.art-ca3fe4aaf9544ae09fa4d216d08b4b30 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-12T11:15:06Z |
| publishDate | 2019-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-ca3fe4aaf9544ae09fa4d216d08b4b302023-09-02T02:12:47ZengMDPI AGAntioxidants2076-39212019-07-018720510.3390/antiox8070205antiox8070205Optimization of Ultrasound-Assisted Extraction of Polyphenols from <i>Myrtus communis</i> L. PericarpNadia Bouaoudia-Madi0Lila Boulekbache-Makhlouf1Khodir Madani2Artur M.S. Silva3Sofiane Dairi4Sonia Oukhmanou–Bensidhoum5Susana M. Cardoso6Laboratoire de Biomathématiques, Biophysique, Biochimie, et Scientométrie (L3BS), Faculté des Sciences de la Natureet de la Vie, Université de Bejaia, 06000 Bejaia, AlgeriaLaboratoire de Biomathématiques, Biophysique, Biochimie, et Scientométrie (L3BS), Faculté des Sciences de la Natureet de la Vie, Université de Bejaia, 06000 Bejaia, AlgeriaLaboratoire de Biomathématiques, Biophysique, Biochimie, et Scientométrie (L3BS), Faculté des Sciences de la Natureet de la Vie, Université de Bejaia, 06000 Bejaia, AlgeriaQOPNA & LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugalLaboratoire de Biomathématiques, Biophysique, Biochimie, et Scientométrie (L3BS), Faculté des Sciences de la Natureet de la Vie, Université de Bejaia, 06000 Bejaia, AlgeriaLaboratoire de Biomathématiques, Biophysique, Biochimie, et Scientométrie (L3BS), Faculté des Sciences de la Natureet de la Vie, Université de Bejaia, 06000 Bejaia, AlgeriaQOPNA & LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugalResponse surface methodology (RSM) was used to optimize the extraction of phenolics from pericap of <i>Myrtus communis</i> using ultrasound-assisted extraction (UAE). The results were compared with those obtained by microwave-assisted extraction (MAE) and conventional solvent extraction (CSE) methods. The individual compounds of the optimized extract obtained by UAE were identified by ultra-high-performance liquid chromatography coupled with diode array detection and electrospray ionization mass spectrometry (UHPLC-DAD-ESI-MS<sup>n</sup>). The yield of total phenolic compounds (TPC) was affected more significantly by ethanol concentration, irradiation time, liquid solvent-to-solid ratio (<i>p</i> < 0.0001) and amplitude (<i>p</i> = 0.0421) and optimal parameters conditions set by the RSM model were 70% (<i>v</i>/<i>v</i>), 7.5 min and 30%, respectively. The experimental yield of TPC (241.66 ± 12.77 mg gallic acid equivalent/g dry weight) confirmed the predicted value (235.52 ± 9.9 mg gallic acid equivalent/g dry weight), allowing also to confirm the model validity. Under optimized conditions, UAE was more efficient than MAE and CSE in extracting antioxidants, which comprised mostly myricetin glycosides. Globally, the present work demonstrated that, compared to MAE and CSE, UAE is an efficient method for phenolic extraction from <i>M. communis</i> pericarp, enabling to reduce the working time and the solvent consumption.https://www.mdpi.com/2076-3921/8/7/205myrtleultrasound-assisted extractionphenolic compoundsantioxidant capacityliquid chromatography analysismass spectrometry |
| spellingShingle | Nadia Bouaoudia-Madi Lila Boulekbache-Makhlouf Khodir Madani Artur M.S. Silva Sofiane Dairi Sonia Oukhmanou–Bensidhoum Susana M. Cardoso Optimization of Ultrasound-Assisted Extraction of Polyphenols from <i>Myrtus communis</i> L. Pericarp Antioxidants myrtle ultrasound-assisted extraction phenolic compounds antioxidant capacity liquid chromatography analysis mass spectrometry |
| title | Optimization of Ultrasound-Assisted Extraction of Polyphenols from <i>Myrtus communis</i> L. Pericarp |
| title_full | Optimization of Ultrasound-Assisted Extraction of Polyphenols from <i>Myrtus communis</i> L. Pericarp |
| title_fullStr | Optimization of Ultrasound-Assisted Extraction of Polyphenols from <i>Myrtus communis</i> L. Pericarp |
| title_full_unstemmed | Optimization of Ultrasound-Assisted Extraction of Polyphenols from <i>Myrtus communis</i> L. Pericarp |
| title_short | Optimization of Ultrasound-Assisted Extraction of Polyphenols from <i>Myrtus communis</i> L. Pericarp |
| title_sort | optimization of ultrasound assisted extraction of polyphenols from i myrtus communis i l pericarp |
| topic | myrtle ultrasound-assisted extraction phenolic compounds antioxidant capacity liquid chromatography analysis mass spectrometry |
| url | https://www.mdpi.com/2076-3921/8/7/205 |
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