Adipose-Derived Mesenchymal Stem Cells-Conditioned Medium Modulates the Expression of Inflammation Induced Bone Morphogenetic Protein-2, -5 and -6 as Well as Compared with Shockwave Therapy on Rat Knee Osteoarthritis
The dose-dependent effects of adipose-derived mesenchymal stem cell-conditioned medium (ADSC-CM) were compared with those of shockwave (SW) therapy in the treatment of early osteoarthritis (OA). Anterior cruciate ligament transaction (ACLT) with medial meniscectomy (MMx) was performed in rats divide...
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MDPI AG
2021-10-01
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| Online Access: | https://www.mdpi.com/2227-9059/9/10/1399 |
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| author | Jai-Hong Cheng Chieh-Cheng Hsu Shan-Ling Hsu Wen-Yi Chou Yi-No Wu Chun-En Aurea Kuo Tsai-Chin Hsu Li-Yen Shiu Shun-Wun Jhan |
| author_facet | Jai-Hong Cheng Chieh-Cheng Hsu Shan-Ling Hsu Wen-Yi Chou Yi-No Wu Chun-En Aurea Kuo Tsai-Chin Hsu Li-Yen Shiu Shun-Wun Jhan |
| author_sort | Jai-Hong Cheng |
| collection | DOAJ |
| description | The dose-dependent effects of adipose-derived mesenchymal stem cell-conditioned medium (ADSC-CM) were compared with those of shockwave (SW) therapy in the treatment of early osteoarthritis (OA). Anterior cruciate ligament transaction (ACLT) with medial meniscectomy (MMx) was performed in rats divided into sham, OA, SW, CM1 (intra-articular injection of 100 μL ADSC-CM into knee OA), and CM2 (intra-articular injection of 200 μL ADSC-CM) groups. Cartilage grading, grading of synovium changes, and specific molecular analysis by immunohistochemistry staining were performed. The OARSI and synovitis scores of CM2 and SW group were significantly decreased compared with those of the OA group (<i>p</i> < 0.05). The inflammatory markers interleukin 1β, terminal deoxynucleotidyl transferase dUTP nick end labeling and matrix metalloproteinase 13 were significantly reduced in the CM2 group compared to those in the SW and CM1 groups (<i>p</i> < 0.001). Cartilage repair markers (type II collagen and SRY-box transcription factor 9, SOX9) expression were significantly higher in the CM2 group than in the other treatment groups (<i>p</i> < 0.001; <i>p</i> < 0.05). Furthermore, inflammation-induced growth factors such as bone morphogenetic protein 2 (BMP2), BMP5, and BMP6 were significantly reduced in the treatment groups, and the CM2 group showed the best results among the treatments (<i>p</i> < 0.05). In conclusion, ADSC-CM and SW ameliorated the expression of inflammatory cytokines and inflammation-induced BMPs to protect the articular cartilage of the OA joint. |
| first_indexed | 2024-03-10T06:42:45Z |
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| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T06:42:45Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
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| series | Biomedicines |
| spelling | doaj.art-ca41aaf347ec452da5387dfb1a2802cd2023-11-22T17:31:18ZengMDPI AGBiomedicines2227-90592021-10-01910139910.3390/biomedicines9101399Adipose-Derived Mesenchymal Stem Cells-Conditioned Medium Modulates the Expression of Inflammation Induced Bone Morphogenetic Protein-2, -5 and -6 as Well as Compared with Shockwave Therapy on Rat Knee OsteoarthritisJai-Hong Cheng0Chieh-Cheng Hsu1Shan-Ling Hsu2Wen-Yi Chou3Yi-No Wu4Chun-En Aurea Kuo5Tsai-Chin Hsu6Li-Yen Shiu7Shun-Wun Jhan8Center for Shockwave Medicine and Tissue Engineering, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 833, TaiwanCenter for Shockwave Medicine and Tissue Engineering, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 833, TaiwanCenter for Shockwave Medicine and Tissue Engineering, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 833, TaiwanCenter for Shockwave Medicine and Tissue Engineering, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 833, TaiwanSchool of Medicine, Fu Jen Catholic University, New Taipei City 242, TaiwanDepartment of Chinese Medicine, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 833, TaiwanCenter for Shockwave Medicine and Tissue Engineering, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 833, TaiwanCell Therapy Center, E-Da Hospital, Kaohsiung 824, TaiwanCenter for Shockwave Medicine and Tissue Engineering, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung 833, TaiwanThe dose-dependent effects of adipose-derived mesenchymal stem cell-conditioned medium (ADSC-CM) were compared with those of shockwave (SW) therapy in the treatment of early osteoarthritis (OA). Anterior cruciate ligament transaction (ACLT) with medial meniscectomy (MMx) was performed in rats divided into sham, OA, SW, CM1 (intra-articular injection of 100 μL ADSC-CM into knee OA), and CM2 (intra-articular injection of 200 μL ADSC-CM) groups. Cartilage grading, grading of synovium changes, and specific molecular analysis by immunohistochemistry staining were performed. The OARSI and synovitis scores of CM2 and SW group were significantly decreased compared with those of the OA group (<i>p</i> < 0.05). The inflammatory markers interleukin 1β, terminal deoxynucleotidyl transferase dUTP nick end labeling and matrix metalloproteinase 13 were significantly reduced in the CM2 group compared to those in the SW and CM1 groups (<i>p</i> < 0.001). Cartilage repair markers (type II collagen and SRY-box transcription factor 9, SOX9) expression were significantly higher in the CM2 group than in the other treatment groups (<i>p</i> < 0.001; <i>p</i> < 0.05). Furthermore, inflammation-induced growth factors such as bone morphogenetic protein 2 (BMP2), BMP5, and BMP6 were significantly reduced in the treatment groups, and the CM2 group showed the best results among the treatments (<i>p</i> < 0.05). In conclusion, ADSC-CM and SW ameliorated the expression of inflammatory cytokines and inflammation-induced BMPs to protect the articular cartilage of the OA joint.https://www.mdpi.com/2227-9059/9/10/1399adipose-derived mesenchymal stem cellsconditioned mediumshockwaveosteoarthritiscartilage repair |
| spellingShingle | Jai-Hong Cheng Chieh-Cheng Hsu Shan-Ling Hsu Wen-Yi Chou Yi-No Wu Chun-En Aurea Kuo Tsai-Chin Hsu Li-Yen Shiu Shun-Wun Jhan Adipose-Derived Mesenchymal Stem Cells-Conditioned Medium Modulates the Expression of Inflammation Induced Bone Morphogenetic Protein-2, -5 and -6 as Well as Compared with Shockwave Therapy on Rat Knee Osteoarthritis Biomedicines adipose-derived mesenchymal stem cells conditioned medium shockwave osteoarthritis cartilage repair |
| title | Adipose-Derived Mesenchymal Stem Cells-Conditioned Medium Modulates the Expression of Inflammation Induced Bone Morphogenetic Protein-2, -5 and -6 as Well as Compared with Shockwave Therapy on Rat Knee Osteoarthritis |
| title_full | Adipose-Derived Mesenchymal Stem Cells-Conditioned Medium Modulates the Expression of Inflammation Induced Bone Morphogenetic Protein-2, -5 and -6 as Well as Compared with Shockwave Therapy on Rat Knee Osteoarthritis |
| title_fullStr | Adipose-Derived Mesenchymal Stem Cells-Conditioned Medium Modulates the Expression of Inflammation Induced Bone Morphogenetic Protein-2, -5 and -6 as Well as Compared with Shockwave Therapy on Rat Knee Osteoarthritis |
| title_full_unstemmed | Adipose-Derived Mesenchymal Stem Cells-Conditioned Medium Modulates the Expression of Inflammation Induced Bone Morphogenetic Protein-2, -5 and -6 as Well as Compared with Shockwave Therapy on Rat Knee Osteoarthritis |
| title_short | Adipose-Derived Mesenchymal Stem Cells-Conditioned Medium Modulates the Expression of Inflammation Induced Bone Morphogenetic Protein-2, -5 and -6 as Well as Compared with Shockwave Therapy on Rat Knee Osteoarthritis |
| title_sort | adipose derived mesenchymal stem cells conditioned medium modulates the expression of inflammation induced bone morphogenetic protein 2 5 and 6 as well as compared with shockwave therapy on rat knee osteoarthritis |
| topic | adipose-derived mesenchymal stem cells conditioned medium shockwave osteoarthritis cartilage repair |
| url | https://www.mdpi.com/2227-9059/9/10/1399 |
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