Numerical Simulation of a Scanning Illumination System for Deep Tissue Fluorescence Imaging
The spatial resolution and light detected in fluorescence imaging for small animals are limited by light scattering, absorption and autofluorescence. To address this, novel near-infrared fluorescent contrast agents and imaging configurations have been investigated. In this paper, the influence of th...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-10-01
|
| Series: | Journal of Imaging |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2313-433X/5/11/83 |
| _version_ | 1818176970026909696 |
|---|---|
| author | Qimei Zhang Anna M. Grabowska Philip A. Clarke Stephen P. Morgan |
| author_facet | Qimei Zhang Anna M. Grabowska Philip A. Clarke Stephen P. Morgan |
| author_sort | Qimei Zhang |
| collection | DOAJ |
| description | The spatial resolution and light detected in fluorescence imaging for small animals are limited by light scattering, absorption and autofluorescence. To address this, novel near-infrared fluorescent contrast agents and imaging configurations have been investigated. In this paper, the influence of the light wavelength and imaging configurations (full-field illumination system and scanning system) on fluorescence imaging are compared quantitatively. The surface radiance for both systems is calculated by modifying the simulation tool Near-Infrared Fluorescence and Spectral Tomography. Fluorescent targets are embedded within a scattering medium at different positions. The surface radiance and spatial resolution are obtained for emission wavelengths between 620 nm and 1000 nm. It was found that the spatial resolution of the scanning system is independent of the tissue optical properties, whereas for full-field illumination, the spatial resolution degrades at longer wavelength. The full width at half maximum obtained by the scanning system is 25% lower than that obtained by the full-field illumination system when the targets are located in the middle of the phantom. The results indicate that although imaging at near-infrared wavelength can achieve a higher surface radiance, it may produce worse spatial resolution. |
| first_indexed | 2024-12-11T20:24:38Z |
| format | Article |
| id | doaj.art-ca41f188cd56471f9e4fe61cde891cd0 |
| institution | Directory Open Access Journal |
| issn | 2313-433X |
| language | English |
| last_indexed | 2024-12-11T20:24:38Z |
| publishDate | 2019-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Imaging |
| spelling | doaj.art-ca41f188cd56471f9e4fe61cde891cd02022-12-22T00:51:59ZengMDPI AGJournal of Imaging2313-433X2019-10-015118310.3390/jimaging5110083jimaging5110083Numerical Simulation of a Scanning Illumination System for Deep Tissue Fluorescence ImagingQimei Zhang0Anna M. Grabowska1Philip A. Clarke2Stephen P. Morgan3Department of Engineering, Nottingham Trent University, Nottingham NG118PR, UKSchool of Medicine, Division of Cancer and Stem Cells, University of Nottingham, Nottingham NG72RD, UKSchool of Medicine, Division of Cancer and Stem Cells, University of Nottingham, Nottingham NG72RD, UKOptics and Photonics Group, Faculty of Engineering, University of Nottingham, Nottingham NG72RD, UKThe spatial resolution and light detected in fluorescence imaging for small animals are limited by light scattering, absorption and autofluorescence. To address this, novel near-infrared fluorescent contrast agents and imaging configurations have been investigated. In this paper, the influence of the light wavelength and imaging configurations (full-field illumination system and scanning system) on fluorescence imaging are compared quantitatively. The surface radiance for both systems is calculated by modifying the simulation tool Near-Infrared Fluorescence and Spectral Tomography. Fluorescent targets are embedded within a scattering medium at different positions. The surface radiance and spatial resolution are obtained for emission wavelengths between 620 nm and 1000 nm. It was found that the spatial resolution of the scanning system is independent of the tissue optical properties, whereas for full-field illumination, the spatial resolution degrades at longer wavelength. The full width at half maximum obtained by the scanning system is 25% lower than that obtained by the full-field illumination system when the targets are located in the middle of the phantom. The results indicate that although imaging at near-infrared wavelength can achieve a higher surface radiance, it may produce worse spatial resolution.https://www.mdpi.com/2313-433X/5/11/83fluorescence imagingresolutionfull-field illuminationnear infrared fluorescence and spectral tomography |
| spellingShingle | Qimei Zhang Anna M. Grabowska Philip A. Clarke Stephen P. Morgan Numerical Simulation of a Scanning Illumination System for Deep Tissue Fluorescence Imaging Journal of Imaging fluorescence imaging resolution full-field illumination near infrared fluorescence and spectral tomography |
| title | Numerical Simulation of a Scanning Illumination System for Deep Tissue Fluorescence Imaging |
| title_full | Numerical Simulation of a Scanning Illumination System for Deep Tissue Fluorescence Imaging |
| title_fullStr | Numerical Simulation of a Scanning Illumination System for Deep Tissue Fluorescence Imaging |
| title_full_unstemmed | Numerical Simulation of a Scanning Illumination System for Deep Tissue Fluorescence Imaging |
| title_short | Numerical Simulation of a Scanning Illumination System for Deep Tissue Fluorescence Imaging |
| title_sort | numerical simulation of a scanning illumination system for deep tissue fluorescence imaging |
| topic | fluorescence imaging resolution full-field illumination near infrared fluorescence and spectral tomography |
| url | https://www.mdpi.com/2313-433X/5/11/83 |
| work_keys_str_mv | AT qimeizhang numericalsimulationofascanningilluminationsystemfordeeptissuefluorescenceimaging AT annamgrabowska numericalsimulationofascanningilluminationsystemfordeeptissuefluorescenceimaging AT philipaclarke numericalsimulationofascanningilluminationsystemfordeeptissuefluorescenceimaging AT stephenpmorgan numericalsimulationofascanningilluminationsystemfordeeptissuefluorescenceimaging |