Molecular characterization of Trypanosoma cruzi samples derived from Triatoma vitticeps and Panstrongylus geniculatus of the Atlantic rainforest, southeast Brazil
Background: In rural areas of Espírito Santo state, southeast Brazil, triatomine species attracted by light frequently invade residences. The aim of this study was to investigate the Trypanosoma cruzi discrete typing units (DTUs) harbored by these triatomines. Methods: Triatomine’s intestinal conten...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
EDP Sciences
2018-01-01
|
| Series: | Parasite |
| Subjects: | |
| Online Access: | https://doi.org/10.1051/parasite/2018060 |
| _version_ | 1797642257535336448 |
|---|---|
| author | Dario Maria Augusta Andrade Tassiane Emanuelle Servare dos Santos Claudiney Biral Fux Blima Brandão Adeilton Alves Falqueto Aloísio |
| author_facet | Dario Maria Augusta Andrade Tassiane Emanuelle Servare dos Santos Claudiney Biral Fux Blima Brandão Adeilton Alves Falqueto Aloísio |
| author_sort | Dario Maria Augusta |
| collection | DOAJ |
| description | Background: In rural areas of Espírito Santo state, southeast Brazil, triatomine species attracted by light frequently invade residences. The aim of this study was to investigate the Trypanosoma cruzi discrete typing units (DTUs) harbored by these triatomines. Methods: Triatomine’s intestinal contents were examined, inoculated in mice, and the positive samples were cultivated. Flagellates obtained from infected mice hemoculture were submitted to DNA extraction using a salting-out method and to TcSC5D gene amplification. The amplified samples were sequenced, and polymorphism was analyzed for DTU identification. Results: Three hundred and ninety-four triatomines were identified: Triatoma vitticeps (90.03%), Panstrongylus geniculatus (8.89%), Panstrongylus megistus (0.54%), Panstrongylus diasi (0.27%), and Triatoma tibiamaculata (0.27%). Among the specimens, 251/394 (67.65%) presented flagellated forms similar to T. cruzi. After triatomine intestinal content inoculation into mice, 134 mice presented T. cruzi-like trypomastigotes from Tr. vitticeps and P. geniculatus and 89 samples were positive in hemoculture. Sixty-two samples were analyzed for the TcSC5D gene and TcI, TcII, TcIII, and TcIV DTUs were identified. Conclusions: We observed T. cruzi DTU diversity in Tr. vitticeps and P. geniculatus, which showed the predominance of TcII and occurrence of TcI, TcIII and TcIV. Triatomines presented high T. cruzi infection rates. Since little is known regarding the possible mammalian hosts that maintain the T. cruzi cycle, further studies are necessary to obtain a better understanding of the parasite transmission cycle in this region. |
| first_indexed | 2024-03-11T13:58:29Z |
| format | Article |
| id | doaj.art-ca42bd3fd4fa4af88a1d3c87447c073e |
| institution | Directory Open Access Journal |
| issn | 1776-1042 |
| language | English |
| last_indexed | 2024-03-11T13:58:29Z |
| publishDate | 2018-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | Parasite |
| spelling | doaj.art-ca42bd3fd4fa4af88a1d3c87447c073e2023-11-02T05:54:00ZengEDP SciencesParasite1776-10422018-01-01255910.1051/parasite/2018060parasite180074Molecular characterization of Trypanosoma cruzi samples derived from Triatoma vitticeps and Panstrongylus geniculatus of the Atlantic rainforest, southeast BrazilDario Maria AugustaAndrade Tassiane Emanuelle Servaredos Santos Claudiney BiralFux BlimaBrandão Adeilton AlvesFalqueto AloísioBackground: In rural areas of Espírito Santo state, southeast Brazil, triatomine species attracted by light frequently invade residences. The aim of this study was to investigate the Trypanosoma cruzi discrete typing units (DTUs) harbored by these triatomines. Methods: Triatomine’s intestinal contents were examined, inoculated in mice, and the positive samples were cultivated. Flagellates obtained from infected mice hemoculture were submitted to DNA extraction using a salting-out method and to TcSC5D gene amplification. The amplified samples were sequenced, and polymorphism was analyzed for DTU identification. Results: Three hundred and ninety-four triatomines were identified: Triatoma vitticeps (90.03%), Panstrongylus geniculatus (8.89%), Panstrongylus megistus (0.54%), Panstrongylus diasi (0.27%), and Triatoma tibiamaculata (0.27%). Among the specimens, 251/394 (67.65%) presented flagellated forms similar to T. cruzi. After triatomine intestinal content inoculation into mice, 134 mice presented T. cruzi-like trypomastigotes from Tr. vitticeps and P. geniculatus and 89 samples were positive in hemoculture. Sixty-two samples were analyzed for the TcSC5D gene and TcI, TcII, TcIII, and TcIV DTUs were identified. Conclusions: We observed T. cruzi DTU diversity in Tr. vitticeps and P. geniculatus, which showed the predominance of TcII and occurrence of TcI, TcIII and TcIV. Triatomines presented high T. cruzi infection rates. Since little is known regarding the possible mammalian hosts that maintain the T. cruzi cycle, further studies are necessary to obtain a better understanding of the parasite transmission cycle in this region.https://doi.org/10.1051/parasite/2018060Trypanosoma cruzi discrete typing unitsmolecular epidemiologyTriatominaeAtlantic rainforestBrazil |
| spellingShingle | Dario Maria Augusta Andrade Tassiane Emanuelle Servare dos Santos Claudiney Biral Fux Blima Brandão Adeilton Alves Falqueto Aloísio Molecular characterization of Trypanosoma cruzi samples derived from Triatoma vitticeps and Panstrongylus geniculatus of the Atlantic rainforest, southeast Brazil Parasite Trypanosoma cruzi discrete typing units molecular epidemiology Triatominae Atlantic rainforest Brazil |
| title | Molecular characterization of Trypanosoma cruzi samples derived from Triatoma vitticeps and Panstrongylus geniculatus of the Atlantic rainforest, southeast Brazil |
| title_full | Molecular characterization of Trypanosoma cruzi samples derived from Triatoma vitticeps and Panstrongylus geniculatus of the Atlantic rainforest, southeast Brazil |
| title_fullStr | Molecular characterization of Trypanosoma cruzi samples derived from Triatoma vitticeps and Panstrongylus geniculatus of the Atlantic rainforest, southeast Brazil |
| title_full_unstemmed | Molecular characterization of Trypanosoma cruzi samples derived from Triatoma vitticeps and Panstrongylus geniculatus of the Atlantic rainforest, southeast Brazil |
| title_short | Molecular characterization of Trypanosoma cruzi samples derived from Triatoma vitticeps and Panstrongylus geniculatus of the Atlantic rainforest, southeast Brazil |
| title_sort | molecular characterization of trypanosoma cruzi samples derived from triatoma vitticeps and panstrongylus geniculatus of the atlantic rainforest southeast brazil |
| topic | Trypanosoma cruzi discrete typing units molecular epidemiology Triatominae Atlantic rainforest Brazil |
| url | https://doi.org/10.1051/parasite/2018060 |
| work_keys_str_mv | AT dariomariaaugusta molecularcharacterizationoftrypanosomacruzisamplesderivedfromtriatomavitticepsandpanstrongylusgeniculatusoftheatlanticrainforestsoutheastbrazil AT andradetassianeemanuelleservare molecularcharacterizationoftrypanosomacruzisamplesderivedfromtriatomavitticepsandpanstrongylusgeniculatusoftheatlanticrainforestsoutheastbrazil AT dossantosclaudineybiral molecularcharacterizationoftrypanosomacruzisamplesderivedfromtriatomavitticepsandpanstrongylusgeniculatusoftheatlanticrainforestsoutheastbrazil AT fuxblima molecularcharacterizationoftrypanosomacruzisamplesderivedfromtriatomavitticepsandpanstrongylusgeniculatusoftheatlanticrainforestsoutheastbrazil AT brandaoadeiltonalves molecularcharacterizationoftrypanosomacruzisamplesderivedfromtriatomavitticepsandpanstrongylusgeniculatusoftheatlanticrainforestsoutheastbrazil AT falquetoaloisio molecularcharacterizationoftrypanosomacruzisamplesderivedfromtriatomavitticepsandpanstrongylusgeniculatusoftheatlanticrainforestsoutheastbrazil |