Selection of Reference Genes for Expression Analysis in Chinese Medicinal Herb Huperzia serrata
Huperzine A (HupA) is a powerful and selective inhibitor of acetylcholinesterase. It has attracted widespread attention endangering the ultimate plant sources of Lycopodiaceae family. In this study, we used Huperzia serrata, extensively used in Traditional Chinese medicine (TCM), a slow growing vasc...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2019-02-01
|
| Series: | Frontiers in Pharmacology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fphar.2019.00044/full |
| _version_ | 1819232080017489920 |
|---|---|
| author | Mengquan Yang Mengquan Yang Shiwen Wu Wenjing You Wenjing You Amit Jaisi Youli Xiao Youli Xiao Youli Xiao |
| author_facet | Mengquan Yang Mengquan Yang Shiwen Wu Wenjing You Wenjing You Amit Jaisi Youli Xiao Youli Xiao Youli Xiao |
| author_sort | Mengquan Yang |
| collection | DOAJ |
| description | Huperzine A (HupA) is a powerful and selective inhibitor of acetylcholinesterase. It has attracted widespread attention endangering the ultimate plant sources of Lycopodiaceae family. In this study, we used Huperzia serrata, extensively used in Traditional Chinese medicine (TCM), a slow growing vascular plant as the model plant of the Lycopodiaceae family to develop and validate the reference genes. We aim to use gene expression platform to understand the gene expression of different tissues and developmental stages of this medicinal herb. Eight candidate reference genes were selected based on RNA-seq data and evaluated with qRT-PCR. The expression of L/ODC and cytochrome P450s genes known for their involvement in lycopodium alkaloid biosynthesis, were also studied to validate the selected reference genes. The most stable genes were TBP, GAPDH, and their combination (TBP + GAPDH). We report for the first time the reference gene of H. serrata’s different tissues which would provide important insights into understanding their biological functions comparing other Lycopodiaceae plants and facilitate a good biopharming approach. |
| first_indexed | 2024-12-23T11:55:10Z |
| format | Article |
| id | doaj.art-ca450eafbde64613b121c4d096b1e563 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-23T11:55:10Z |
| publishDate | 2019-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-ca450eafbde64613b121c4d096b1e5632022-12-21T17:48:06ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122019-02-011010.3389/fphar.2019.00044421316Selection of Reference Genes for Expression Analysis in Chinese Medicinal Herb Huperzia serrataMengquan Yang0Mengquan Yang1Shiwen Wu2Wenjing You3Wenjing You4Amit Jaisi5Youli Xiao6Youli Xiao7Youli Xiao8CAS Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaCAS Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, ChinaCAS Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaCAS Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, ChinaCAS Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaCAS-JIC Centre of Excellence in Plant and Microbial Sciences, Shanghai, ChinaHuperzine A (HupA) is a powerful and selective inhibitor of acetylcholinesterase. It has attracted widespread attention endangering the ultimate plant sources of Lycopodiaceae family. In this study, we used Huperzia serrata, extensively used in Traditional Chinese medicine (TCM), a slow growing vascular plant as the model plant of the Lycopodiaceae family to develop and validate the reference genes. We aim to use gene expression platform to understand the gene expression of different tissues and developmental stages of this medicinal herb. Eight candidate reference genes were selected based on RNA-seq data and evaluated with qRT-PCR. The expression of L/ODC and cytochrome P450s genes known for their involvement in lycopodium alkaloid biosynthesis, were also studied to validate the selected reference genes. The most stable genes were TBP, GAPDH, and their combination (TBP + GAPDH). We report for the first time the reference gene of H. serrata’s different tissues which would provide important insights into understanding their biological functions comparing other Lycopodiaceae plants and facilitate a good biopharming approach.https://www.frontiersin.org/article/10.3389/fphar.2019.00044/fullHuperzia serratalycopodium alkaloidsreference genereal-time quantitative PCRgene expression |
| spellingShingle | Mengquan Yang Mengquan Yang Shiwen Wu Wenjing You Wenjing You Amit Jaisi Youli Xiao Youli Xiao Youli Xiao Selection of Reference Genes for Expression Analysis in Chinese Medicinal Herb Huperzia serrata Frontiers in Pharmacology Huperzia serrata lycopodium alkaloids reference gene real-time quantitative PCR gene expression |
| title | Selection of Reference Genes for Expression Analysis in Chinese Medicinal Herb Huperzia serrata |
| title_full | Selection of Reference Genes for Expression Analysis in Chinese Medicinal Herb Huperzia serrata |
| title_fullStr | Selection of Reference Genes for Expression Analysis in Chinese Medicinal Herb Huperzia serrata |
| title_full_unstemmed | Selection of Reference Genes for Expression Analysis in Chinese Medicinal Herb Huperzia serrata |
| title_short | Selection of Reference Genes for Expression Analysis in Chinese Medicinal Herb Huperzia serrata |
| title_sort | selection of reference genes for expression analysis in chinese medicinal herb huperzia serrata |
| topic | Huperzia serrata lycopodium alkaloids reference gene real-time quantitative PCR gene expression |
| url | https://www.frontiersin.org/article/10.3389/fphar.2019.00044/full |
| work_keys_str_mv | AT mengquanyang selectionofreferencegenesforexpressionanalysisinchinesemedicinalherbhuperziaserrata AT mengquanyang selectionofreferencegenesforexpressionanalysisinchinesemedicinalherbhuperziaserrata AT shiwenwu selectionofreferencegenesforexpressionanalysisinchinesemedicinalherbhuperziaserrata AT wenjingyou selectionofreferencegenesforexpressionanalysisinchinesemedicinalherbhuperziaserrata AT wenjingyou selectionofreferencegenesforexpressionanalysisinchinesemedicinalherbhuperziaserrata AT amitjaisi selectionofreferencegenesforexpressionanalysisinchinesemedicinalherbhuperziaserrata AT youlixiao selectionofreferencegenesforexpressionanalysisinchinesemedicinalherbhuperziaserrata AT youlixiao selectionofreferencegenesforexpressionanalysisinchinesemedicinalherbhuperziaserrata AT youlixiao selectionofreferencegenesforexpressionanalysisinchinesemedicinalherbhuperziaserrata |