Regulatory Effects of <i>FGF9</i> on Dermal Papilla Cell Proliferation in Small-Tailed Han Sheep
Fibroblast growth factor 9 (<i>FGF9</i>) is crucial for the growth and development of hair follicles (HFs); however, its role in sheep wool growth is unknown. Here, we clarified the role of <i>FGF9</i> in HF growth in the small-tailed Han sheep by quantifying <i>FGF9<...
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MDPI AG
2023-05-01
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| Online Access: | https://www.mdpi.com/2073-4425/14/5/1106 |
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| author | Qi Jia Shuangshuang Zhang Dan Wang Jianqiang Liu Xinhui Luo Yu Liu Xin Li Fuliang Sun Guangjun Xia Lichun Zhang |
| author_facet | Qi Jia Shuangshuang Zhang Dan Wang Jianqiang Liu Xinhui Luo Yu Liu Xin Li Fuliang Sun Guangjun Xia Lichun Zhang |
| author_sort | Qi Jia |
| collection | DOAJ |
| description | Fibroblast growth factor 9 (<i>FGF9</i>) is crucial for the growth and development of hair follicles (HFs); however, its role in sheep wool growth is unknown. Here, we clarified the role of <i>FGF9</i> in HF growth in the small-tailed Han sheep by quantifying <i>FGF9</i> expression in skin tissue sections collected at different periods. Moreover, we evaluated the effects of <i>FGF9</i> protein supplementation on hair shaft growth in vitro and <i>FGF9</i> knockdown on cultured dermal papilla cells (DPCs). The relationship between <i>FGF9</i> and the Wnt/β-catenin signaling pathway was examined, and the underlying mechanisms of <i>FGF9</i>-mediated DPC proliferation were investigated. The results show that <i>FGF9</i> expression varies throughout the HF cycle and participates in wool growth. The proliferation rate and cell cycle of <i>FGF9</i>-treated DPCs substantially increase compared to that of the control group, and the mRNA and protein expression of <i>CTNNB1</i>, a Wnt/β-catenin signaling pathway marker gene, is considerably lower than that in the control group. The opposite occurs in <i>FGF9</i>-knockdown DPCs. Moreover, other signaling pathways are enriched in the <i>FGF9</i>-treated group. In conclusion, <i>FGF9</i> accelerates the proliferation and cell cycle of DPCs and may regulate HF growth and development through the Wnt/β-catenin signaling pathway. |
| first_indexed | 2024-03-11T03:41:57Z |
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| last_indexed | 2024-03-11T03:41:57Z |
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| series | Genes |
| spelling | doaj.art-ca4722df944f4c9fa4182efca994850f2023-11-18T01:30:33ZengMDPI AGGenes2073-44252023-05-01145110610.3390/genes14051106Regulatory Effects of <i>FGF9</i> on Dermal Papilla Cell Proliferation in Small-Tailed Han SheepQi Jia0Shuangshuang Zhang1Dan Wang2Jianqiang Liu3Xinhui Luo4Yu Liu5Xin Li6Fuliang Sun7Guangjun Xia8Lichun Zhang9Institute of Animal Biotechnology, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, ChinaInstitute of Animal Biotechnology, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, ChinaCollege of Agriculture, Yanbian University, Yanji 130021, ChinaInstitute of Animal Biotechnology, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, ChinaInstitute of Animal Biotechnology, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, ChinaInstitute of Animal Biotechnology, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, ChinaInstitute of Animal Biotechnology, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, ChinaCollege of Agriculture, Yanbian University, Yanji 130021, ChinaCollege of Agriculture, Yanbian University, Yanji 130021, ChinaInstitute of Animal Biotechnology, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, ChinaFibroblast growth factor 9 (<i>FGF9</i>) is crucial for the growth and development of hair follicles (HFs); however, its role in sheep wool growth is unknown. Here, we clarified the role of <i>FGF9</i> in HF growth in the small-tailed Han sheep by quantifying <i>FGF9</i> expression in skin tissue sections collected at different periods. Moreover, we evaluated the effects of <i>FGF9</i> protein supplementation on hair shaft growth in vitro and <i>FGF9</i> knockdown on cultured dermal papilla cells (DPCs). The relationship between <i>FGF9</i> and the Wnt/β-catenin signaling pathway was examined, and the underlying mechanisms of <i>FGF9</i>-mediated DPC proliferation were investigated. The results show that <i>FGF9</i> expression varies throughout the HF cycle and participates in wool growth. The proliferation rate and cell cycle of <i>FGF9</i>-treated DPCs substantially increase compared to that of the control group, and the mRNA and protein expression of <i>CTNNB1</i>, a Wnt/β-catenin signaling pathway marker gene, is considerably lower than that in the control group. The opposite occurs in <i>FGF9</i>-knockdown DPCs. Moreover, other signaling pathways are enriched in the <i>FGF9</i>-treated group. In conclusion, <i>FGF9</i> accelerates the proliferation and cell cycle of DPCs and may regulate HF growth and development through the Wnt/β-catenin signaling pathway.https://www.mdpi.com/2073-4425/14/5/1106fibroblast growth factor 9small-tailed Han sheepdermal papilla cellsproliferation |
| spellingShingle | Qi Jia Shuangshuang Zhang Dan Wang Jianqiang Liu Xinhui Luo Yu Liu Xin Li Fuliang Sun Guangjun Xia Lichun Zhang Regulatory Effects of <i>FGF9</i> on Dermal Papilla Cell Proliferation in Small-Tailed Han Sheep Genes fibroblast growth factor 9 small-tailed Han sheep dermal papilla cells proliferation |
| title | Regulatory Effects of <i>FGF9</i> on Dermal Papilla Cell Proliferation in Small-Tailed Han Sheep |
| title_full | Regulatory Effects of <i>FGF9</i> on Dermal Papilla Cell Proliferation in Small-Tailed Han Sheep |
| title_fullStr | Regulatory Effects of <i>FGF9</i> on Dermal Papilla Cell Proliferation in Small-Tailed Han Sheep |
| title_full_unstemmed | Regulatory Effects of <i>FGF9</i> on Dermal Papilla Cell Proliferation in Small-Tailed Han Sheep |
| title_short | Regulatory Effects of <i>FGF9</i> on Dermal Papilla Cell Proliferation in Small-Tailed Han Sheep |
| title_sort | regulatory effects of i fgf9 i on dermal papilla cell proliferation in small tailed han sheep |
| topic | fibroblast growth factor 9 small-tailed Han sheep dermal papilla cells proliferation |
| url | https://www.mdpi.com/2073-4425/14/5/1106 |
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