Use of native chicken breeds (Gallus gallus domesticus) for the development of suitable methods of Cantabrian capercaillie (Tetrao urogallus cantabricus) semen cryopreservation
Abstract Background The Cantabrian capercaillie (Tetrao urogallus cantabricus) is critically endangered. This subspecies has the lowest genetic variability and it is in regression. It belongs to Phasianidae family; therefore, the domestic chicken (Gallus gallus domesticus) could be a good model for...
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| Format: | Article |
| Language: | English |
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Wiley
2022-05-01
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| Series: | Veterinary Medicine and Science |
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| Online Access: | https://doi.org/10.1002/vms3.742 |
| _version_ | 1797683437664993280 |
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| author | Emma O'Brien Cristina Castaño Adolfo Toledano‐Díaz José Néstor Caamaño Carlos Hidalgo Luis Eusebio Fidalgo Ana María López‐Beceiro Milagros Cristina Esteso Ramón Balsera Pedro García‐Casado Ewa Łukaszewicz Julián Santiago‐Moreno |
| author_facet | Emma O'Brien Cristina Castaño Adolfo Toledano‐Díaz José Néstor Caamaño Carlos Hidalgo Luis Eusebio Fidalgo Ana María López‐Beceiro Milagros Cristina Esteso Ramón Balsera Pedro García‐Casado Ewa Łukaszewicz Julián Santiago‐Moreno |
| author_sort | Emma O'Brien |
| collection | DOAJ |
| description | Abstract Background The Cantabrian capercaillie (Tetrao urogallus cantabricus) is critically endangered. This subspecies has the lowest genetic variability and it is in regression. It belongs to Phasianidae family; therefore, the domestic chicken (Gallus gallus domesticus) could be a good model for developing reproductive technologies for use in capercaillie populations with low availability of animals. Objectives In this study, we analyzed the response of capercaillie sperm to the freezing–thawing process for contributing to the development of a semen cryobank of Cantabrian capercaillie. Methods We used domestic chicken as the animal model in order to obtain the freezing protocol before applying on capercaillie. In the first experiment, two different extenders (EK and LR84) and different concentrations [4% and 6% dimethyl‐acetamide (DMA) v:v] of cryoprotectants were evaluated using in‐straw freezing method in domestic chickens. A pilot study in capercaillie males, using the same conditions evaluated in chicken, was performed. Results In chicken, we found that the LR84‐4% DMA media provided the best results for freezing semen. In capercaillie study, LR84 extender seemed to be the most appropriate diluent and 4% was the better dose of DMA cryoprotectant agent. Further, based on previous studies carried out in rooster samples, we also tested the glycerol (8% v/v) as a cryoprotectant for capercaillie semen cryopreservation. Conclusions Our results suggest that sperm from both domestic and wild species had a similar response to freezing–thawing processes. Mediterranean chickens may be used as a suitable model for developing sperm freezing protocols that can be extrapolated to threatened capercaillie populations. In addition, LR84 media with glycerol was the most efficient extender to freeze capercaillie sperm native. |
| first_indexed | 2024-03-12T00:15:18Z |
| format | Article |
| id | doaj.art-ca49231797cf435d930ec9c6412d13d0 |
| institution | Directory Open Access Journal |
| issn | 2053-1095 |
| language | English |
| last_indexed | 2024-03-12T00:15:18Z |
| publishDate | 2022-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj.art-ca49231797cf435d930ec9c6412d13d02023-09-15T15:20:44ZengWileyVeterinary Medicine and Science2053-10952022-05-01831311131810.1002/vms3.742Use of native chicken breeds (Gallus gallus domesticus) for the development of suitable methods of Cantabrian capercaillie (Tetrao urogallus cantabricus) semen cryopreservationEmma O'Brien0Cristina Castaño1Adolfo Toledano‐Díaz2José Néstor Caamaño3Carlos Hidalgo4Luis Eusebio Fidalgo5Ana María López‐Beceiro6Milagros Cristina Esteso7Ramón Balsera8Pedro García‐Casado9Ewa Łukaszewicz10Julián Santiago‐Moreno11Departmento de Reproducción Animal Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA‐CSIC) Madrid SpainDepartmento de Reproducción Animal Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA‐CSIC) Madrid SpainDepartmento de Reproducción Animal Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA‐CSIC) Madrid SpainSelección y Reproducción Animal‐SERIDA Principado de Asturias Gijón SpainSelección y Reproducción Animal‐SERIDA Principado de Asturias Gijón SpainDepartment of Anatomy Animal Production and Veterinary Clinical Sciences Faculty of Veterinary Universidad de Santiago de Compostela Lugo SpainDepartment of Anatomy Animal Production and Veterinary Clinical Sciences Faculty of Veterinary Universidad de Santiago de Compostela Lugo SpainDepartmento de Reproducción Animal Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA‐CSIC) Madrid SpainServicio de Vigilancia y Control de la Biodiversidad Dirección General de Medio Natural Oviedo SpainR&D Department Zoitechlab (Arquimea Group) Madrid SpainDivision of Poultry Breeding Institute of Animal Breeding Wrocław University of Environmental and Life Sciences Wrocław PolandDepartmento de Reproducción Animal Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA‐CSIC) Madrid SpainAbstract Background The Cantabrian capercaillie (Tetrao urogallus cantabricus) is critically endangered. This subspecies has the lowest genetic variability and it is in regression. It belongs to Phasianidae family; therefore, the domestic chicken (Gallus gallus domesticus) could be a good model for developing reproductive technologies for use in capercaillie populations with low availability of animals. Objectives In this study, we analyzed the response of capercaillie sperm to the freezing–thawing process for contributing to the development of a semen cryobank of Cantabrian capercaillie. Methods We used domestic chicken as the animal model in order to obtain the freezing protocol before applying on capercaillie. In the first experiment, two different extenders (EK and LR84) and different concentrations [4% and 6% dimethyl‐acetamide (DMA) v:v] of cryoprotectants were evaluated using in‐straw freezing method in domestic chickens. A pilot study in capercaillie males, using the same conditions evaluated in chicken, was performed. Results In chicken, we found that the LR84‐4% DMA media provided the best results for freezing semen. In capercaillie study, LR84 extender seemed to be the most appropriate diluent and 4% was the better dose of DMA cryoprotectant agent. Further, based on previous studies carried out in rooster samples, we also tested the glycerol (8% v/v) as a cryoprotectant for capercaillie semen cryopreservation. Conclusions Our results suggest that sperm from both domestic and wild species had a similar response to freezing–thawing processes. Mediterranean chickens may be used as a suitable model for developing sperm freezing protocols that can be extrapolated to threatened capercaillie populations. In addition, LR84 media with glycerol was the most efficient extender to freeze capercaillie sperm native.https://doi.org/10.1002/vms3.742capercailliecryoprotectantextendersperm freezing |
| spellingShingle | Emma O'Brien Cristina Castaño Adolfo Toledano‐Díaz José Néstor Caamaño Carlos Hidalgo Luis Eusebio Fidalgo Ana María López‐Beceiro Milagros Cristina Esteso Ramón Balsera Pedro García‐Casado Ewa Łukaszewicz Julián Santiago‐Moreno Use of native chicken breeds (Gallus gallus domesticus) for the development of suitable methods of Cantabrian capercaillie (Tetrao urogallus cantabricus) semen cryopreservation Veterinary Medicine and Science capercaillie cryoprotectant extender sperm freezing |
| title | Use of native chicken breeds (Gallus gallus domesticus) for the development of suitable methods of Cantabrian capercaillie (Tetrao urogallus cantabricus) semen cryopreservation |
| title_full | Use of native chicken breeds (Gallus gallus domesticus) for the development of suitable methods of Cantabrian capercaillie (Tetrao urogallus cantabricus) semen cryopreservation |
| title_fullStr | Use of native chicken breeds (Gallus gallus domesticus) for the development of suitable methods of Cantabrian capercaillie (Tetrao urogallus cantabricus) semen cryopreservation |
| title_full_unstemmed | Use of native chicken breeds (Gallus gallus domesticus) for the development of suitable methods of Cantabrian capercaillie (Tetrao urogallus cantabricus) semen cryopreservation |
| title_short | Use of native chicken breeds (Gallus gallus domesticus) for the development of suitable methods of Cantabrian capercaillie (Tetrao urogallus cantabricus) semen cryopreservation |
| title_sort | use of native chicken breeds gallus gallus domesticus for the development of suitable methods of cantabrian capercaillie tetrao urogallus cantabricus semen cryopreservation |
| topic | capercaillie cryoprotectant extender sperm freezing |
| url | https://doi.org/10.1002/vms3.742 |
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