In vitro degradation, haemolysis and cytotoxicity study of Mg‐0.4Ce/ZnO2 nanocomposites
Abstract Magnesium is an ideal candidate for biodegradable implants, but the major concern is its uncontrollable degradation for application as a biomaterial. The in vitro corrosion and cytotoxicity of Mg‐0.4Ce/ZnO2 (magnesium nanocomposites) were studied to determine its suitability as a biodegrada...
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| Format: | Article |
| Language: | English |
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Hindawi-IET
2021-04-01
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| Series: | IET Nanobiotechnology |
| Online Access: | https://doi.org/10.1049/nbt2.12032 |
| _version_ | 1797428897059438592 |
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| author | Meenachi Prabakaran Subashini Rajakannu Lakshminarayanan K Adhimoolam Manoj Gupta |
| author_facet | Meenachi Prabakaran Subashini Rajakannu Lakshminarayanan K Adhimoolam Manoj Gupta |
| author_sort | Meenachi Prabakaran |
| collection | DOAJ |
| description | Abstract Magnesium is an ideal candidate for biodegradable implants, but the major concern is its uncontrollable degradation for application as a biomaterial. The in vitro corrosion and cytotoxicity of Mg‐0.4Ce/ZnO2 (magnesium nanocomposites) were studied to determine its suitability as a biodegradable material. The polycrystalline nature of Mg‐0.4Ce/ZnO2 was assessed using an optical microscope. The hydrophobic nature of Mg‐0.4Ce/ZnO2 was determined by contact angle measurements. The corrosion resistance of magnesium nanocomposites was tested in phosphate buffer solution (PBS) and it was improved by the gradual deposition of a protective layer on its surface after 48 h. The cytotoxicity of Mg‐0.4Ce/ZnO2 was evaluated by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay and calcium deposition by Alizarin red staining using sarcoma osteogenic (Saos2) cells. The haemocompatibility test of Mg‐0.4Ce/ZnO2 showed 30% haemolysis, which is higher than the safe value for biomaterials, and cell viability was reduced after 24 h in comparison with control groups. The calcium deposition by sarcoma osteogenic cells showed a brick red colour deposition in both the control group and Mg‐0.4Ce/ZnO2 after 24 h. The preliminary degradation results of Mg‐0.4Ce/ZnO2 showed good corrosion resistance; however further improvement is needed in haemolysis and cytotoxicity studies for its use as a biodegradable material for orthopaedic applications. |
| first_indexed | 2024-03-09T09:05:28Z |
| format | Article |
| id | doaj.art-ca49ace94f5844ba8e1fcfb82bb0129f |
| institution | Directory Open Access Journal |
| issn | 1751-8741 1751-875X |
| language | English |
| last_indexed | 2024-03-09T09:05:28Z |
| publishDate | 2021-04-01 |
| publisher | Hindawi-IET |
| record_format | Article |
| series | IET Nanobiotechnology |
| spelling | doaj.art-ca49ace94f5844ba8e1fcfb82bb0129f2023-12-02T10:39:49ZengHindawi-IETIET Nanobiotechnology1751-87411751-875X2021-04-0115215716310.1049/nbt2.12032In vitro degradation, haemolysis and cytotoxicity study of Mg‐0.4Ce/ZnO2 nanocompositesMeenachi Prabakaran0Subashini Rajakannu1Lakshminarayanan K Adhimoolam2Manoj Gupta3Department of Biomedical Engineering SSN College of Engineering Chennai IndiaDepartment of Biomedical Engineering SSN College of Engineering Chennai IndiaDepartment of Mechanical Engineering SSN College of Engineering Chennai IndiaDepartment of Mechanical Engineering National University of Singapore Singapore SingaporeAbstract Magnesium is an ideal candidate for biodegradable implants, but the major concern is its uncontrollable degradation for application as a biomaterial. The in vitro corrosion and cytotoxicity of Mg‐0.4Ce/ZnO2 (magnesium nanocomposites) were studied to determine its suitability as a biodegradable material. The polycrystalline nature of Mg‐0.4Ce/ZnO2 was assessed using an optical microscope. The hydrophobic nature of Mg‐0.4Ce/ZnO2 was determined by contact angle measurements. The corrosion resistance of magnesium nanocomposites was tested in phosphate buffer solution (PBS) and it was improved by the gradual deposition of a protective layer on its surface after 48 h. The cytotoxicity of Mg‐0.4Ce/ZnO2 was evaluated by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay and calcium deposition by Alizarin red staining using sarcoma osteogenic (Saos2) cells. The haemocompatibility test of Mg‐0.4Ce/ZnO2 showed 30% haemolysis, which is higher than the safe value for biomaterials, and cell viability was reduced after 24 h in comparison with control groups. The calcium deposition by sarcoma osteogenic cells showed a brick red colour deposition in both the control group and Mg‐0.4Ce/ZnO2 after 24 h. The preliminary degradation results of Mg‐0.4Ce/ZnO2 showed good corrosion resistance; however further improvement is needed in haemolysis and cytotoxicity studies for its use as a biodegradable material for orthopaedic applications.https://doi.org/10.1049/nbt2.12032 |
| spellingShingle | Meenachi Prabakaran Subashini Rajakannu Lakshminarayanan K Adhimoolam Manoj Gupta In vitro degradation, haemolysis and cytotoxicity study of Mg‐0.4Ce/ZnO2 nanocomposites IET Nanobiotechnology |
| title | In vitro degradation, haemolysis and cytotoxicity study of Mg‐0.4Ce/ZnO2 nanocomposites |
| title_full | In vitro degradation, haemolysis and cytotoxicity study of Mg‐0.4Ce/ZnO2 nanocomposites |
| title_fullStr | In vitro degradation, haemolysis and cytotoxicity study of Mg‐0.4Ce/ZnO2 nanocomposites |
| title_full_unstemmed | In vitro degradation, haemolysis and cytotoxicity study of Mg‐0.4Ce/ZnO2 nanocomposites |
| title_short | In vitro degradation, haemolysis and cytotoxicity study of Mg‐0.4Ce/ZnO2 nanocomposites |
| title_sort | in vitro degradation haemolysis and cytotoxicity study of mg 0 4ce zno2 nanocomposites |
| url | https://doi.org/10.1049/nbt2.12032 |
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