Development of a 2D-HPLC for simultaneous determination of rhynchophylline， isorhynchophylline， corynoxeine and isocorynoxeine
ObjectiveTo develop a two-dimensional high-performance liquid chromatography （2D-HPLC） for simultaneous determination of the contents of four kinds of Uncaria alkaloids： rhynchophylline， isorhynchophylline， corynoxeine and isocorynoxeine.MethodsThe 2D-HPLC apparatus was comprised of a first chromato...
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| Format: | Article |
| Language: | zho |
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Editorial Office of Sichuan Mental Health
2022-10-01
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| Series: | Sichuan jingshen weisheng |
| Subjects: | |
| Online Access: | http://www.psychjm.net.cn/scjswszzen/ch/reader/view_abstract.aspx?file_no=202205006&flag=1 |
| _version_ | 1797627628757188608 |
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| author | Zhang Yan Zhang Suo Wei Wei Su Yanli Ding Jing Zhao Caiping Dai Zunxiao Wang Hui |
| author_facet | Zhang Yan Zhang Suo Wei Wei Su Yanli Ding Jing Zhao Caiping Dai Zunxiao Wang Hui |
| author_sort | Zhang Yan |
| collection | DOAJ |
| description | ObjectiveTo develop a two-dimensional high-performance liquid chromatography （2D-HPLC） for simultaneous determination of the contents of four kinds of Uncaria alkaloids： rhynchophylline， isorhynchophylline， corynoxeine and isocorynoxeine.MethodsThe 2D-HPLC apparatus was comprised of a first chromatographic column in version Aston SC2 （3.5 mm×25 mm， 5 μm）， an intermediate column in version Aston SH C18 （3.5 mm×10 mm， 5 μm）， and an analytical column in version Aston SCB （4.6 mm×125 mm， 5 μm）. The mobile phase of the first and second liquid chromatography system were CAA-1 and mixed mobile phase （V BPI-1 basic mobile phase ∶ V MPI-1 mobile phase ∶ V OPI-1 organic mobile phase = 45∶14∶41）. The chromatographic parameters included a flow rate of 1.0 mL/min， a column temperature of 40℃， a wavelength of 254 nm， an injection volume of 500 μL and a detection time of 9.5 min.ResultsThe linear ranges of rhynchophylline， isorhynchophylline， corynoxeine and isocorynoxeine were 9.77~10 000.00 ng/mL （r=0.999 6）， 10.74~11 000.00 ng/mL （r=0.999 7）， 10.74~11 000.00 ng/mL （r=0.999 7）， 10.74~11 000.00 ng/mL（r=0.999 6）， respectively. The relative standard deviation （RSD） of precision， stability and repeatability were all less than 5.00%. The accuracy was 95.20%~104.01%， and the recovery rate was 93.63%~101.38%.ConclusionThe 2D-HPLC developed for simultaneous determination of four kinds of alkaloids in Uncaria is simple and accurate， which can be used as a new method for quality control of Uncaria. |
| first_indexed | 2024-03-11T10:27:01Z |
| format | Article |
| id | doaj.art-ca4ce361a8c2474882f5be9b47cb349c |
| institution | Directory Open Access Journal |
| issn | 1007-3256 |
| language | zho |
| last_indexed | 2024-03-11T10:27:01Z |
| publishDate | 2022-10-01 |
| publisher | Editorial Office of Sichuan Mental Health |
| record_format | Article |
| series | Sichuan jingshen weisheng |
| spelling | doaj.art-ca4ce361a8c2474882f5be9b47cb349c2023-11-15T12:28:04ZzhoEditorial Office of Sichuan Mental HealthSichuan jingshen weisheng1007-32562022-10-0135542442810.11886/scjsws202201080011007-3256（2022）05-0424-05Development of a 2D-HPLC for simultaneous determination of rhynchophylline， isorhynchophylline， corynoxeine and isocorynoxeineZhang Yan0Zhang Suo1Wei Wei2Su Yanli3Ding Jing4Zhao Caiping5Dai Zunxiao6Wang Hui7Xi'an Mental Health Center， Xi'an 710100， ChinaXi'an Mental Health Center， Xi'an 710100， ChinaXi'an Mental Health Center， Xi'an 710100， ChinaXi'an Mental Health Center， Xi'an 710100， ChinaXi'an Mental Health Center， Xi'an 710100， ChinaXi'an Mental Health Center， Xi'an 710100， ChinaXi'an Mental Health Center， Xi'an 710100， ChinaXi'an Mental Health Center， Xi'an 710100， ChinaObjectiveTo develop a two-dimensional high-performance liquid chromatography （2D-HPLC） for simultaneous determination of the contents of four kinds of Uncaria alkaloids： rhynchophylline， isorhynchophylline， corynoxeine and isocorynoxeine.MethodsThe 2D-HPLC apparatus was comprised of a first chromatographic column in version Aston SC2 （3.5 mm×25 mm， 5 μm）， an intermediate column in version Aston SH C18 （3.5 mm×10 mm， 5 μm）， and an analytical column in version Aston SCB （4.6 mm×125 mm， 5 μm）. The mobile phase of the first and second liquid chromatography system were CAA-1 and mixed mobile phase （V BPI-1 basic mobile phase ∶ V MPI-1 mobile phase ∶ V OPI-1 organic mobile phase = 45∶14∶41）. The chromatographic parameters included a flow rate of 1.0 mL/min， a column temperature of 40℃， a wavelength of 254 nm， an injection volume of 500 μL and a detection time of 9.5 min.ResultsThe linear ranges of rhynchophylline， isorhynchophylline， corynoxeine and isocorynoxeine were 9.77~10 000.00 ng/mL （r=0.999 6）， 10.74~11 000.00 ng/mL （r=0.999 7）， 10.74~11 000.00 ng/mL （r=0.999 7）， 10.74~11 000.00 ng/mL（r=0.999 6）， respectively. The relative standard deviation （RSD） of precision， stability and repeatability were all less than 5.00%. The accuracy was 95.20%~104.01%， and the recovery rate was 93.63%~101.38%.ConclusionThe 2D-HPLC developed for simultaneous determination of four kinds of alkaloids in Uncaria is simple and accurate， which can be used as a new method for quality control of Uncaria.http://www.psychjm.net.cn/scjswszzen/ch/reader/view_abstract.aspx?file_no=202205006&flag=12d-hplcrhynchophyllineisorhynchophyllinecorynoxeineisocorynoxeine |
| spellingShingle | Zhang Yan Zhang Suo Wei Wei Su Yanli Ding Jing Zhao Caiping Dai Zunxiao Wang Hui Development of a 2D-HPLC for simultaneous determination of rhynchophylline， isorhynchophylline， corynoxeine and isocorynoxeine Sichuan jingshen weisheng 2d-hplc rhynchophylline isorhynchophylline corynoxeine isocorynoxeine |
| title | Development of a 2D-HPLC for simultaneous determination of rhynchophylline， isorhynchophylline， corynoxeine and isocorynoxeine |
| title_full | Development of a 2D-HPLC for simultaneous determination of rhynchophylline， isorhynchophylline， corynoxeine and isocorynoxeine |
| title_fullStr | Development of a 2D-HPLC for simultaneous determination of rhynchophylline， isorhynchophylline， corynoxeine and isocorynoxeine |
| title_full_unstemmed | Development of a 2D-HPLC for simultaneous determination of rhynchophylline， isorhynchophylline， corynoxeine and isocorynoxeine |
| title_short | Development of a 2D-HPLC for simultaneous determination of rhynchophylline， isorhynchophylline， corynoxeine and isocorynoxeine |
| title_sort | development of a 2d hplc for simultaneous determination of rhynchophylline isorhynchophylline corynoxeine and isocorynoxeine |
| topic | 2d-hplc rhynchophylline isorhynchophylline corynoxeine isocorynoxeine |
| url | http://www.psychjm.net.cn/scjswszzen/ch/reader/view_abstract.aspx?file_no=202205006&flag=1 |
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