Extracellular vesicles secreted by human gingival mesenchymal stem cells promote bone regeneration in rat femoral bone defects
Extracellular vesicles (EVs), important components of paracrine secretion, are involved in various pathological and physiological processes of the body. In this study, we researched the benefits of EVs secreted by human gingival mesenchymal stem cells (hGMSC-derived EVs) in promoting bone regenerati...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1098172/full |
| _version_ | 1797901826055471104 |
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| author | Situo Wang Situo Wang Ziwei Liu Ziwei Liu Ziwei Liu Shuo Yang Na Huo Bo Qiao Tong Zhang Juan Xu Quan Shi |
| author_facet | Situo Wang Situo Wang Ziwei Liu Ziwei Liu Ziwei Liu Shuo Yang Na Huo Bo Qiao Tong Zhang Juan Xu Quan Shi |
| author_sort | Situo Wang |
| collection | DOAJ |
| description | Extracellular vesicles (EVs), important components of paracrine secretion, are involved in various pathological and physiological processes of the body. In this study, we researched the benefits of EVs secreted by human gingival mesenchymal stem cells (hGMSC-derived EVs) in promoting bone regeneration, thereby providing new ideas for EVs-based bone regeneration therapy. Here, we successfully demonstrated that hGMSC-derived EVs could enhance the osteogenic ability of rat bone marrow mesenchymal stem cells and the angiogenic capability of human umbilical vein endothelial cells. Then, femoral defect rat models were created and treated with phosphate-buffered saline, nanohydroxyapatite/collagen (nHAC), a grouping of nHAC/hGMSCs, and a grouping of nHAC/EVs. The results of our study indicated that the combination of hGMSC-derived EVs and nHAC materials could significantly promote new bone formation and neovascularization with a similar effect to that of the nHAC/hGMSCs group. Our outcomes provide new messages on the role of hGMSC-derived EVs in tissue engineering, which exhibit great potential in bone regeneration treatment. |
| first_indexed | 2024-04-10T09:08:04Z |
| format | Article |
| id | doaj.art-ca4f00802a4849cfafddeb3795d85ad4 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-10T09:08:04Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-ca4f00802a4849cfafddeb3795d85ad42023-02-21T06:13:47ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-02-011110.3389/fbioe.2023.10981721098172Extracellular vesicles secreted by human gingival mesenchymal stem cells promote bone regeneration in rat femoral bone defectsSituo Wang0Situo Wang1Ziwei Liu2Ziwei Liu3Ziwei Liu4Shuo Yang5Na Huo6Bo Qiao7Tong Zhang8Juan Xu9Quan Shi10Department of Stomatology, The First Medical Center, Chinese PLA General Hospital, Beijing, ChinaOrthopedic Laboratory of PLA General Hospital, Beijing, ChinaDepartment of Stomatology, The First Medical Center, Chinese PLA General Hospital, Beijing, ChinaOrthopedic Laboratory of PLA General Hospital, Beijing, ChinaMedical School of Chinese PLA, Beijing, ChinaDepartment of Stomatology, The First Medical Center, Chinese PLA General Hospital, Beijing, ChinaDepartment of Stomatology, The First Medical Center, Chinese PLA General Hospital, Beijing, ChinaDepartment of Stomatology, The First Medical Center, Chinese PLA General Hospital, Beijing, ChinaDepartment of Stomatology, The First Medical Center, Chinese PLA General Hospital, Beijing, ChinaDepartment of Stomatology, The First Medical Center, Chinese PLA General Hospital, Beijing, ChinaDepartment of Stomatology, The First Medical Center, Chinese PLA General Hospital, Beijing, ChinaExtracellular vesicles (EVs), important components of paracrine secretion, are involved in various pathological and physiological processes of the body. In this study, we researched the benefits of EVs secreted by human gingival mesenchymal stem cells (hGMSC-derived EVs) in promoting bone regeneration, thereby providing new ideas for EVs-based bone regeneration therapy. Here, we successfully demonstrated that hGMSC-derived EVs could enhance the osteogenic ability of rat bone marrow mesenchymal stem cells and the angiogenic capability of human umbilical vein endothelial cells. Then, femoral defect rat models were created and treated with phosphate-buffered saline, nanohydroxyapatite/collagen (nHAC), a grouping of nHAC/hGMSCs, and a grouping of nHAC/EVs. The results of our study indicated that the combination of hGMSC-derived EVs and nHAC materials could significantly promote new bone formation and neovascularization with a similar effect to that of the nHAC/hGMSCs group. Our outcomes provide new messages on the role of hGMSC-derived EVs in tissue engineering, which exhibit great potential in bone regeneration treatment.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1098172/fullextracellular vesicleshuman gingival mesenchymal stem cellsosteogenic abilityangiogenic capabilitybone regeneration |
| spellingShingle | Situo Wang Situo Wang Ziwei Liu Ziwei Liu Ziwei Liu Shuo Yang Na Huo Bo Qiao Tong Zhang Juan Xu Quan Shi Extracellular vesicles secreted by human gingival mesenchymal stem cells promote bone regeneration in rat femoral bone defects Frontiers in Bioengineering and Biotechnology extracellular vesicles human gingival mesenchymal stem cells osteogenic ability angiogenic capability bone regeneration |
| title | Extracellular vesicles secreted by human gingival mesenchymal stem cells promote bone regeneration in rat femoral bone defects |
| title_full | Extracellular vesicles secreted by human gingival mesenchymal stem cells promote bone regeneration in rat femoral bone defects |
| title_fullStr | Extracellular vesicles secreted by human gingival mesenchymal stem cells promote bone regeneration in rat femoral bone defects |
| title_full_unstemmed | Extracellular vesicles secreted by human gingival mesenchymal stem cells promote bone regeneration in rat femoral bone defects |
| title_short | Extracellular vesicles secreted by human gingival mesenchymal stem cells promote bone regeneration in rat femoral bone defects |
| title_sort | extracellular vesicles secreted by human gingival mesenchymal stem cells promote bone regeneration in rat femoral bone defects |
| topic | extracellular vesicles human gingival mesenchymal stem cells osteogenic ability angiogenic capability bone regeneration |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1098172/full |
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