Oral Lactobacillus species and their probiotic capabilities in patients with periodontitis and periodontally healthy individuals
Abstract Objectives This study aimed to identify oral Lactobacillus species and characterize their adhesion properties and antibacterial activity in patients with periodontitis compared with periodontally healthy individuals. Materials and Methods Three hundred and fifty‐four isolates from the saliv...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-10-01
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| Series: | Clinical and Experimental Dental Research |
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| Online Access: | https://doi.org/10.1002/cre2.740 |
| _version_ | 1797657908393017344 |
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| author | Arghavan Etebarian Tahere Sheshpari Kourosh Kabir Hanieh Sadeghi Abouzar Moradi Avin Hafedi |
| author_facet | Arghavan Etebarian Tahere Sheshpari Kourosh Kabir Hanieh Sadeghi Abouzar Moradi Avin Hafedi |
| author_sort | Arghavan Etebarian |
| collection | DOAJ |
| description | Abstract Objectives This study aimed to identify oral Lactobacillus species and characterize their adhesion properties and antibacterial activity in patients with periodontitis compared with periodontally healthy individuals. Materials and Methods Three hundred and fifty‐four isolates from the saliva, subgingival, and tongue plaque of 59 periodontitis patients and 59 healthy individuals were analyzed. Oral Lactobacillus species were identified through the culture method in the modified MRS medium and confirmed by molecular testing. Moreover, the radial diffusion assay and cell culture methods were used to determine the antibacterial activities of oral strains against oral pathogens and their adhesion activity in vitro. Results 67.7% of the cases and 75.7% of the control samples were positive for the Lactobacillus species. Lacticaseibacillus paracasei and Limosilactobacillus fermentum were the dominant species in the case group, whereas Lacticaseibacillus casei and Lactiplantibacillus plantarum were dominant in the control group. Lactobacillus crispatus and Lactobacillus gasseri had higher antibacterial effects against oral pathogens. Moreover, Ligilactobacillus salivarius and L. fermentum demonstrated the highest ability to adhere to oral mucosal cells and salivary‐coated hydroxyapatite. Conclusion L. crispatus, L. gasseri, L. fermentum, and L. salivarius can be introduced as probiotic candidates since they demonstrated appropriate adherence to oral mucosal cells and salivary‐coated hydroxyapatite and also antibacterial activities. However, further studies should be conducted to assess the safety of probiotic interventions using these strains in patients with periodontal disease. |
| first_indexed | 2024-03-11T17:52:21Z |
| format | Article |
| id | doaj.art-ca4f8e349edf432db41b87a98febb8ac |
| institution | Directory Open Access Journal |
| issn | 2057-4347 |
| language | English |
| last_indexed | 2024-03-11T17:52:21Z |
| publishDate | 2023-10-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Experimental Dental Research |
| spelling | doaj.art-ca4f8e349edf432db41b87a98febb8ac2023-10-18T03:37:29ZengWileyClinical and Experimental Dental Research2057-43472023-10-019574675610.1002/cre2.740Oral Lactobacillus species and their probiotic capabilities in patients with periodontitis and periodontally healthy individualsArghavan Etebarian0Tahere Sheshpari1Kourosh Kabir2Hanieh Sadeghi3Abouzar Moradi4Avin Hafedi5Oral and Maxillofacial Pathology Department, School of Dentistry Alborz University of Medical Sciences Karaj IranMicrobiology Department, School of Medicine Alborz University of Medical Sciences Karaj IranCommunity Medicine Department, Dietary Supplements and Probiotic Research Center Alborz University of Medical Sciences Karaj IranStudent Research Committee Alborz University of Medical Sciences Karaj IranPeriodontology Department, School of Dentistry Alborz University of Medical Sciences Karaj IranStudent Research Committee Alborz University of Medical Sciences Karaj IranAbstract Objectives This study aimed to identify oral Lactobacillus species and characterize their adhesion properties and antibacterial activity in patients with periodontitis compared with periodontally healthy individuals. Materials and Methods Three hundred and fifty‐four isolates from the saliva, subgingival, and tongue plaque of 59 periodontitis patients and 59 healthy individuals were analyzed. Oral Lactobacillus species were identified through the culture method in the modified MRS medium and confirmed by molecular testing. Moreover, the radial diffusion assay and cell culture methods were used to determine the antibacterial activities of oral strains against oral pathogens and their adhesion activity in vitro. Results 67.7% of the cases and 75.7% of the control samples were positive for the Lactobacillus species. Lacticaseibacillus paracasei and Limosilactobacillus fermentum were the dominant species in the case group, whereas Lacticaseibacillus casei and Lactiplantibacillus plantarum were dominant in the control group. Lactobacillus crispatus and Lactobacillus gasseri had higher antibacterial effects against oral pathogens. Moreover, Ligilactobacillus salivarius and L. fermentum demonstrated the highest ability to adhere to oral mucosal cells and salivary‐coated hydroxyapatite. Conclusion L. crispatus, L. gasseri, L. fermentum, and L. salivarius can be introduced as probiotic candidates since they demonstrated appropriate adherence to oral mucosal cells and salivary‐coated hydroxyapatite and also antibacterial activities. However, further studies should be conducted to assess the safety of probiotic interventions using these strains in patients with periodontal disease.https://doi.org/10.1002/cre2.740lactobacillusoral cavityperiodontitisprobiotics |
| spellingShingle | Arghavan Etebarian Tahere Sheshpari Kourosh Kabir Hanieh Sadeghi Abouzar Moradi Avin Hafedi Oral Lactobacillus species and their probiotic capabilities in patients with periodontitis and periodontally healthy individuals Clinical and Experimental Dental Research lactobacillus oral cavity periodontitis probiotics |
| title | Oral Lactobacillus species and their probiotic capabilities in patients with periodontitis and periodontally healthy individuals |
| title_full | Oral Lactobacillus species and their probiotic capabilities in patients with periodontitis and periodontally healthy individuals |
| title_fullStr | Oral Lactobacillus species and their probiotic capabilities in patients with periodontitis and periodontally healthy individuals |
| title_full_unstemmed | Oral Lactobacillus species and their probiotic capabilities in patients with periodontitis and periodontally healthy individuals |
| title_short | Oral Lactobacillus species and their probiotic capabilities in patients with periodontitis and periodontally healthy individuals |
| title_sort | oral lactobacillus species and their probiotic capabilities in patients with periodontitis and periodontally healthy individuals |
| topic | lactobacillus oral cavity periodontitis probiotics |
| url | https://doi.org/10.1002/cre2.740 |
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