Thiol-disulfide metabolism in kidney tissue at the administration of some copper coordination compounds
Background: Thiol-disulfide metabolism is essential for normal function of the organism. Thus the interest of the scientists in this area of research continues to grow. Material and methods: Copper coordination compounds (CCC), derivatives of thiosemicarbaside (CMD-4, CMJ-33, CMT-67), action on thio...
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| Format: | Article |
| Language: | English |
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Scientific Medical Association of Moldova
2020-06-01
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| Series: | The Moldovan Medical Journal |
| Subjects: | |
| Online Access: | http://moldmedjournal.md/wp-content/uploads/2020/06/moldovan-med-j-2020-63-2-sardari-et-al-full-text.pdf |
| _version_ | 1818040118090399744 |
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| author | Veronica Sardari Valeriana Pantea Aurelian Gulea Olga Tagadiuc Lilia Andronache Inna Svet Victor Tapcov Valentin Gudumac |
| author_facet | Veronica Sardari Valeriana Pantea Aurelian Gulea Olga Tagadiuc Lilia Andronache Inna Svet Victor Tapcov Valentin Gudumac |
| author_sort | Veronica Sardari |
| collection | DOAJ |
| description | Background: Thiol-disulfide metabolism is essential for normal function of the organism. Thus the interest of the scientists in this area of research continues to grow. Material and methods: Copper coordination compounds (CCC), derivatives of thiosemicarbaside (CMD-4, CMJ-33, CMT-67), action on thiol-disulfide metabolism in the healthy Ratta albicans kidneys were studied. The animals were divided in 6 groups of 7 rats each. The control group included healthy rats which were injected i/m physiological solution 3 times a week, for 30 days. The rats from groups 2-6 have got 3 times a week, for 30 days, i/m injections of CCC. The activity of following thiol-disulfide metabolism enzymes in the renal supernatant has been measured: glutathion-reductase (GR), glutathion-peroxidase (GPO), glutathion-S-transferase (G-S-T), γ-glutamyl transpeptidase (γ-GTP), glutaredoxin (Grx), as well the amount of the protein SH-groups and of the total glutathione, reduced glutathione (GSH) and oxidized glutathione (GSSG) in renal tissue. Results: The compounds exhibit different actions: CMT-67 in the dose of 0.1 µM/kg influenced the activity of the glutathione metabolism enzymes – activated γ-glutamyl transpeptidase (γ-GTP) and glutaredoxine (Grx) and inhibited glutathione reductase (GR), while CMD-4 in doses of 0.1 µM/kg and 1.0 µM/kg and CMJ-33 in the dose of 1.0 µM/kg significantly diminished the reduced glutathione (GSH) level and increased the amount of the oxidised one (GSSG). Conclusions: Selective action of the copper coordination copounds established by this study opens new possibilities of their usage in the therapy of kidney diseases. |
| first_indexed | 2024-12-10T08:09:26Z |
| format | Article |
| id | doaj.art-ca4fa840288741feaca9cb4b101295a1 |
| institution | Directory Open Access Journal |
| issn | 2537-6373 2537-6381 |
| language | English |
| last_indexed | 2024-12-10T08:09:26Z |
| publishDate | 2020-06-01 |
| publisher | Scientific Medical Association of Moldova |
| record_format | Article |
| series | The Moldovan Medical Journal |
| spelling | doaj.art-ca4fa840288741feaca9cb4b101295a12022-12-22T01:56:37ZengScientific Medical Association of MoldovaThe Moldovan Medical Journal2537-63732537-63812020-06-01632121710.5281/zenodo.3865976Thiol-disulfide metabolism in kidney tissue at the administration of some copper coordination compoundsVeronica Sardari0https://orcid.org/0000-0002-1047-9145Valeriana Pantea1https://orcid.org/0000-0002-8835-6612Aurelian Gulea2https://orcid.org/0000-0003-2010-7959Olga Tagadiuc3https://orcid.org/0000-0002-5503-8052Lilia Andronache4https://orcid.org/0000-0002-8781-8037Inna Svet5https://orcid.org/0000-0001-6059-1170Victor Tapcov6https://orcid.org/0000-0003-1732-3116Valentin Gudumac7https://orcid.org/0000-0001-9773-1878Laboratory of Biochemistry, Nicolae Testemitanu State University of Medicine and Pharmacy, Chisinau, the Republic of MoldovaLaboratory of Biochemistry, Nicolae Testemitanu State University of Medicine and Pharmacy, Chisinau, the Republic of MoldovaDepartment of Chemistry, State University of Moldova, Chisinau, the Republic of MoldovaDepartment of Biochemistry and Clinical Biochemistry, Nicolae Testemitanu State University of Medicine and Pharmacy, Chisinau, the Republic of MoldovaLaboratory of Biochemistry, Nicolae Testemitanu State University of Medicine and Pharmacy, Chisinau, the Republic of MoldovaLaboratory of Biochemistry, Nicolae Testemitanu State University of Medicine and Pharmacy, Chisinau, the Republic of MoldovaDepartment of Chemistry, State University of Moldova, Chisinau, the Republic of MoldovaLaboratory of Biochemistry, Nicolae Testemitanu State University of Medicine and Pharmacy, Chisinau, the Republic of MoldovaBackground: Thiol-disulfide metabolism is essential for normal function of the organism. Thus the interest of the scientists in this area of research continues to grow. Material and methods: Copper coordination compounds (CCC), derivatives of thiosemicarbaside (CMD-4, CMJ-33, CMT-67), action on thiol-disulfide metabolism in the healthy Ratta albicans kidneys were studied. The animals were divided in 6 groups of 7 rats each. The control group included healthy rats which were injected i/m physiological solution 3 times a week, for 30 days. The rats from groups 2-6 have got 3 times a week, for 30 days, i/m injections of CCC. The activity of following thiol-disulfide metabolism enzymes in the renal supernatant has been measured: glutathion-reductase (GR), glutathion-peroxidase (GPO), glutathion-S-transferase (G-S-T), γ-glutamyl transpeptidase (γ-GTP), glutaredoxin (Grx), as well the amount of the protein SH-groups and of the total glutathione, reduced glutathione (GSH) and oxidized glutathione (GSSG) in renal tissue. Results: The compounds exhibit different actions: CMT-67 in the dose of 0.1 µM/kg influenced the activity of the glutathione metabolism enzymes – activated γ-glutamyl transpeptidase (γ-GTP) and glutaredoxine (Grx) and inhibited glutathione reductase (GR), while CMD-4 in doses of 0.1 µM/kg and 1.0 µM/kg and CMJ-33 in the dose of 1.0 µM/kg significantly diminished the reduced glutathione (GSH) level and increased the amount of the oxidised one (GSSG). Conclusions: Selective action of the copper coordination copounds established by this study opens new possibilities of their usage in the therapy of kidney diseases.http://moldmedjournal.md/wp-content/uploads/2020/06/moldovan-med-j-2020-63-2-sardari-et-al-full-text.pdfthiol-disulfide metabolismcopper coordination compoundskidney tissue |
| spellingShingle | Veronica Sardari Valeriana Pantea Aurelian Gulea Olga Tagadiuc Lilia Andronache Inna Svet Victor Tapcov Valentin Gudumac Thiol-disulfide metabolism in kidney tissue at the administration of some copper coordination compounds The Moldovan Medical Journal thiol-disulfide metabolism copper coordination compounds kidney tissue |
| title | Thiol-disulfide metabolism in kidney tissue at the administration of some copper coordination compounds |
| title_full | Thiol-disulfide metabolism in kidney tissue at the administration of some copper coordination compounds |
| title_fullStr | Thiol-disulfide metabolism in kidney tissue at the administration of some copper coordination compounds |
| title_full_unstemmed | Thiol-disulfide metabolism in kidney tissue at the administration of some copper coordination compounds |
| title_short | Thiol-disulfide metabolism in kidney tissue at the administration of some copper coordination compounds |
| title_sort | thiol disulfide metabolism in kidney tissue at the administration of some copper coordination compounds |
| topic | thiol-disulfide metabolism copper coordination compounds kidney tissue |
| url | http://moldmedjournal.md/wp-content/uploads/2020/06/moldovan-med-j-2020-63-2-sardari-et-al-full-text.pdf |
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