Methyl Jasmonate Induces Genes Involved in Linalool Accumulation and Increases the Content of Phenolics in Two Iranian Coriander (<i>Coriandrum sativum</i> L.) Ecotypes
The medicinal herb coriander (<i>Coriandrum sativum</i> L.), with a high linalool (LIN) content, is widely recognized for its therapeutic benefits. As a novel report, the goals of this study were to determine how methyl jasmonate (MeJA) affects total phenolic content (TPC), LIN content,...
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MDPI AG
2022-09-01
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| author | Farzad Kianersi Davood Amin Azarm Farzaneh Fatemi Alireza Pour-Aboughadareh Peter Poczai |
| author_facet | Farzad Kianersi Davood Amin Azarm Farzaneh Fatemi Alireza Pour-Aboughadareh Peter Poczai |
| author_sort | Farzad Kianersi |
| collection | DOAJ |
| description | The medicinal herb coriander (<i>Coriandrum sativum</i> L.), with a high linalool (LIN) content, is widely recognized for its therapeutic benefits. As a novel report, the goals of this study were to determine how methyl jasmonate (MeJA) affects total phenolic content (TPC), LIN content, flavonoid content (TFC), and changes in gene expression involved in the linalool biosynthesis pathway (<i>Cs</i><i>γTRPS</i> and <i>CsLINS</i>). Our findings showed that, in comparison to the control samples, MeJA treatment substantially enhanced the TPC, LIN, and TFC content in both ecotypes. Additionally, for both Iranian coriander ecotypes, treatment-induced increases in <i>CsγTRPS</i> and <i>CsLINS</i> expression were connected to LIN accumulation in all treatments. A 24 h treatment with 150 µM MeJA substantially increased the LIN content in the Mashhad and Zanjan ecotypes, which was between 1.48 and 1.69 times greater than that in untreated plants, according to gas chromatography–mass spectrometry (GC-MS) analysis. Our findings demonstrated that MeJA significantly affects the accumulation of LIN, TPC, and TFC in Iranian <i>C. sativum</i> treated with MeJA, which is likely the consequence of gene activation from the monoterpene biosynthesis pathway. Our discoveries have improved the understanding of the molecular mechanisms behind LIN synthesis in coriander plants. |
| first_indexed | 2024-03-09T20:11:03Z |
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| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T20:11:03Z |
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| series | Genes |
| spelling | doaj.art-ca58bf9af16d4ecb9bf0b98ecaf2262d2023-11-24T00:14:53ZengMDPI AGGenes2073-44252022-09-011310171710.3390/genes13101717Methyl Jasmonate Induces Genes Involved in Linalool Accumulation and Increases the Content of Phenolics in Two Iranian Coriander (<i>Coriandrum sativum</i> L.) EcotypesFarzad Kianersi0Davood Amin Azarm1Farzaneh Fatemi2Alireza Pour-Aboughadareh3Peter Poczai4School of Environmental Sciences, University of Guelph, 50 Stone Road East, Guelph, ON N1G 2W1, CanadaDepartment of Horticulture Crop Research, Isfahan Agricultural and Natural Resources Research and Education Center, AREEO, Isfahan P.O. Box 81785-199, IranDepartment of Agronomy and Plant Breeding, Faculty of Agriculture, Bu-Ali Sina University, Hamedan P.O. Box 6517838695, IranSeed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj P.O. Box 3158854119, IranBotany Unit, Finnish Museum of Natural History, University of Helsinki, P.O. Box 7, FI-00014 Helsinki, FinlandThe medicinal herb coriander (<i>Coriandrum sativum</i> L.), with a high linalool (LIN) content, is widely recognized for its therapeutic benefits. As a novel report, the goals of this study were to determine how methyl jasmonate (MeJA) affects total phenolic content (TPC), LIN content, flavonoid content (TFC), and changes in gene expression involved in the linalool biosynthesis pathway (<i>Cs</i><i>γTRPS</i> and <i>CsLINS</i>). Our findings showed that, in comparison to the control samples, MeJA treatment substantially enhanced the TPC, LIN, and TFC content in both ecotypes. Additionally, for both Iranian coriander ecotypes, treatment-induced increases in <i>CsγTRPS</i> and <i>CsLINS</i> expression were connected to LIN accumulation in all treatments. A 24 h treatment with 150 µM MeJA substantially increased the LIN content in the Mashhad and Zanjan ecotypes, which was between 1.48 and 1.69 times greater than that in untreated plants, according to gas chromatography–mass spectrometry (GC-MS) analysis. Our findings demonstrated that MeJA significantly affects the accumulation of LIN, TPC, and TFC in Iranian <i>C. sativum</i> treated with MeJA, which is likely the consequence of gene activation from the monoterpene biosynthesis pathway. Our discoveries have improved the understanding of the molecular mechanisms behind LIN synthesis in coriander plants.https://www.mdpi.com/2073-4425/13/10/1717coriandertotal flavonoid contenttotal phenoliclinaloolmethyl jasmonategene expression |
| spellingShingle | Farzad Kianersi Davood Amin Azarm Farzaneh Fatemi Alireza Pour-Aboughadareh Peter Poczai Methyl Jasmonate Induces Genes Involved in Linalool Accumulation and Increases the Content of Phenolics in Two Iranian Coriander (<i>Coriandrum sativum</i> L.) Ecotypes Genes coriander total flavonoid content total phenolic linalool methyl jasmonate gene expression |
| title | Methyl Jasmonate Induces Genes Involved in Linalool Accumulation and Increases the Content of Phenolics in Two Iranian Coriander (<i>Coriandrum sativum</i> L.) Ecotypes |
| title_full | Methyl Jasmonate Induces Genes Involved in Linalool Accumulation and Increases the Content of Phenolics in Two Iranian Coriander (<i>Coriandrum sativum</i> L.) Ecotypes |
| title_fullStr | Methyl Jasmonate Induces Genes Involved in Linalool Accumulation and Increases the Content of Phenolics in Two Iranian Coriander (<i>Coriandrum sativum</i> L.) Ecotypes |
| title_full_unstemmed | Methyl Jasmonate Induces Genes Involved in Linalool Accumulation and Increases the Content of Phenolics in Two Iranian Coriander (<i>Coriandrum sativum</i> L.) Ecotypes |
| title_short | Methyl Jasmonate Induces Genes Involved in Linalool Accumulation and Increases the Content of Phenolics in Two Iranian Coriander (<i>Coriandrum sativum</i> L.) Ecotypes |
| title_sort | methyl jasmonate induces genes involved in linalool accumulation and increases the content of phenolics in two iranian coriander i coriandrum sativum i l ecotypes |
| topic | coriander total flavonoid content total phenolic linalool methyl jasmonate gene expression |
| url | https://www.mdpi.com/2073-4425/13/10/1717 |
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