Multi-Omics Analysis Reveals Mechanisms of Strong Phosphorus Adaptation in Tea Plant Roots
Low phosphorus (P) is a major limiting factor for plant growth in acid soils, which are preferred by tea plants. This study aims to investigate the unique mechanisms of tea plant roots adaptation to low-P conditions. Tea plant roots were harvested for multi-omics analysis after being treated with 0...
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MDPI AG
2023-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/15/12431 |
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| author | Xiaomei Liu Jing Tian Guodao Liu Lili Sun |
| author_facet | Xiaomei Liu Jing Tian Guodao Liu Lili Sun |
| author_sort | Xiaomei Liu |
| collection | DOAJ |
| description | Low phosphorus (P) is a major limiting factor for plant growth in acid soils, which are preferred by tea plants. This study aims to investigate the unique mechanisms of tea plant roots adaptation to low-P conditions. Tea plant roots were harvested for multi-omics analysis after being treated with 0 µmol·L<sup>−1</sup> P (0P) and 250 µmol·L<sup>−1</sup> P (250P) for 30 days. Under 250P conditions, root elongation was significantly inhibited, and the density of lateral roots was dramatically increased. This suggests that 250P may inhibit the elongation of tea plant roots. Moreover, the P concentration in roots was about 4.58 times higher than that under 0P, indicating that 250P may cause P toxicity in tea plant roots. Contrary to common plants, the expression of <i>CsPT1/2</i> in tea plant roots was significantly increased by four times at 250P, which indicated that tea plant roots suffering from P toxicity might be due to the excessive expression of phosphate uptake-responsible genes under 250P conditions. Additionally, 94.80% of P-containing metabolites accumulated due to 250P stimulation, most of which were energy-associated metabolites, including lipids, nucleotides, and sugars. Especially the ratio of AMP/ATP and the expression of energy sensor <i>CsSnRKs</i> were inhibited by P application. Therefore, under 250P conditions, P over-accumulation due to the excessive expression of <i>CsPT1/2</i> may inhibit energy metabolism and thus the growth of tea plant roots. |
| first_indexed | 2024-03-11T00:24:46Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T00:24:46Z |
| publishDate | 2023-08-01 |
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| spelling | doaj.art-ca58e71b32014302ae50f7ce90870a552023-11-18T23:04:57ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-08-0124151243110.3390/ijms241512431Multi-Omics Analysis Reveals Mechanisms of Strong Phosphorus Adaptation in Tea Plant RootsXiaomei Liu0Jing Tian1Guodao Liu2Lili Sun3College of Tropical Crops, Hainan University, Haikou 570228, ChinaHaixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaInstitute of Tropical Crops Genetic Resources, Chinese Academy of Tropical Agriculture Sciences, Haikou 570228, ChinaHaixia Institute of Science and Technology, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaLow phosphorus (P) is a major limiting factor for plant growth in acid soils, which are preferred by tea plants. This study aims to investigate the unique mechanisms of tea plant roots adaptation to low-P conditions. Tea plant roots were harvested for multi-omics analysis after being treated with 0 µmol·L<sup>−1</sup> P (0P) and 250 µmol·L<sup>−1</sup> P (250P) for 30 days. Under 250P conditions, root elongation was significantly inhibited, and the density of lateral roots was dramatically increased. This suggests that 250P may inhibit the elongation of tea plant roots. Moreover, the P concentration in roots was about 4.58 times higher than that under 0P, indicating that 250P may cause P toxicity in tea plant roots. Contrary to common plants, the expression of <i>CsPT1/2</i> in tea plant roots was significantly increased by four times at 250P, which indicated that tea plant roots suffering from P toxicity might be due to the excessive expression of phosphate uptake-responsible genes under 250P conditions. Additionally, 94.80% of P-containing metabolites accumulated due to 250P stimulation, most of which were energy-associated metabolites, including lipids, nucleotides, and sugars. Especially the ratio of AMP/ATP and the expression of energy sensor <i>CsSnRKs</i> were inhibited by P application. Therefore, under 250P conditions, P over-accumulation due to the excessive expression of <i>CsPT1/2</i> may inhibit energy metabolism and thus the growth of tea plant roots.https://www.mdpi.com/1422-0067/24/15/12431tea plantphosphorusroot growthmulti-omics |
| spellingShingle | Xiaomei Liu Jing Tian Guodao Liu Lili Sun Multi-Omics Analysis Reveals Mechanisms of Strong Phosphorus Adaptation in Tea Plant Roots International Journal of Molecular Sciences tea plant phosphorus root growth multi-omics |
| title | Multi-Omics Analysis Reveals Mechanisms of Strong Phosphorus Adaptation in Tea Plant Roots |
| title_full | Multi-Omics Analysis Reveals Mechanisms of Strong Phosphorus Adaptation in Tea Plant Roots |
| title_fullStr | Multi-Omics Analysis Reveals Mechanisms of Strong Phosphorus Adaptation in Tea Plant Roots |
| title_full_unstemmed | Multi-Omics Analysis Reveals Mechanisms of Strong Phosphorus Adaptation in Tea Plant Roots |
| title_short | Multi-Omics Analysis Reveals Mechanisms of Strong Phosphorus Adaptation in Tea Plant Roots |
| title_sort | multi omics analysis reveals mechanisms of strong phosphorus adaptation in tea plant roots |
| topic | tea plant phosphorus root growth multi-omics |
| url | https://www.mdpi.com/1422-0067/24/15/12431 |
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