Opportunities for isoporous membranes in the manufacture of genomic medicines
Viral and non-viral vectors have revolutionised in the last 5 years the approaches to tackling pandemics, cancers and genetic diseases. The intrinsic properties of these vectors present new separation challenges to their manufacture in terms of both the process-related impurities to be removed and t...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-11-01
|
| Series: | Journal of Membrane Science Letters |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772421223000168 |
| _version_ | 1797397574466928640 |
|---|---|
| author | Ke Meng Thomas F. Johnson Alberto Alvarez-Fernandez Stefan Guldin Daniel G. Bracewell |
| author_facet | Ke Meng Thomas F. Johnson Alberto Alvarez-Fernandez Stefan Guldin Daniel G. Bracewell |
| author_sort | Ke Meng |
| collection | DOAJ |
| description | Viral and non-viral vectors have revolutionised in the last 5 years the approaches to tackling pandemics, cancers and genetic diseases. The intrinsic properties of these vectors present new separation challenges to their manufacture in terms of both the process-related impurities to be removed and the complex labile nature of the target products. These characteristics make them susceptible to heterogeneity and the formation of product-related impurities.Conventional polyethersulfone membrane filters used for sterile filtration and ultrafiltration of viral vectors and lipid nanoparticles can display limited selectivity and cause product losses. To address these challenges, novel membrane materials and fabrication techniques to overcome the boundary of selectivity-permeability performance have become of interest. Isoporous membranes with well-defined pore size and pore dispersity at the nano-scale show promising separation performance but have only been demonstrated at small scales to date.This review summarises the decision process for the development of new membrane candidates for vector manufacturing in genomic medicine, including membranes fabricated by lithography, track-etched membranes, anodic aluminium oxide (AAO) membranes and self-assembled block copolymer membranes. By comparing these membranes to existing commercially available products, the possible advantages presented by novel materials and fabrication approaches are identified. |
| first_indexed | 2024-03-09T01:12:04Z |
| format | Article |
| id | doaj.art-ca5a7f1542184264801d34222641c254 |
| institution | Directory Open Access Journal |
| issn | 2772-4212 |
| language | English |
| last_indexed | 2024-03-09T01:12:04Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Membrane Science Letters |
| spelling | doaj.art-ca5a7f1542184264801d34222641c2542023-12-11T04:17:39ZengElsevierJournal of Membrane Science Letters2772-42122023-11-0132100052Opportunities for isoporous membranes in the manufacture of genomic medicinesKe Meng0Thomas F. Johnson1Alberto Alvarez-Fernandez2Stefan Guldin3Daniel G. Bracewell4Department of Biochemical Engineering, Gower Street, University College London, London, WC1E 6BT, UKDepartment of Biochemical Engineering, Gower Street, University College London, London, WC1E 6BT, UKDepartment of Chemical Engineering, University College London, Torrington Place, London, WC1E 7HB, UKDepartment of Chemical Engineering, University College London, Torrington Place, London, WC1E 7HB, UKDepartment of Biochemical Engineering, Gower Street, University College London, London, WC1E 6BT, UK; Corresponding author at: Department of Biochemical Engineering, Gower Street, University College London, London, WC1E 6BT, UK.Viral and non-viral vectors have revolutionised in the last 5 years the approaches to tackling pandemics, cancers and genetic diseases. The intrinsic properties of these vectors present new separation challenges to their manufacture in terms of both the process-related impurities to be removed and the complex labile nature of the target products. These characteristics make them susceptible to heterogeneity and the formation of product-related impurities.Conventional polyethersulfone membrane filters used for sterile filtration and ultrafiltration of viral vectors and lipid nanoparticles can display limited selectivity and cause product losses. To address these challenges, novel membrane materials and fabrication techniques to overcome the boundary of selectivity-permeability performance have become of interest. Isoporous membranes with well-defined pore size and pore dispersity at the nano-scale show promising separation performance but have only been demonstrated at small scales to date.This review summarises the decision process for the development of new membrane candidates for vector manufacturing in genomic medicine, including membranes fabricated by lithography, track-etched membranes, anodic aluminium oxide (AAO) membranes and self-assembled block copolymer membranes. By comparing these membranes to existing commercially available products, the possible advantages presented by novel materials and fabrication approaches are identified.http://www.sciencedirect.com/science/article/pii/S2772421223000168Isoporous membranesGenomic medicineBio-separationUltrafiltrationBlock copolymer membranes |
| spellingShingle | Ke Meng Thomas F. Johnson Alberto Alvarez-Fernandez Stefan Guldin Daniel G. Bracewell Opportunities for isoporous membranes in the manufacture of genomic medicines Journal of Membrane Science Letters Isoporous membranes Genomic medicine Bio-separation Ultrafiltration Block copolymer membranes |
| title | Opportunities for isoporous membranes in the manufacture of genomic medicines |
| title_full | Opportunities for isoporous membranes in the manufacture of genomic medicines |
| title_fullStr | Opportunities for isoporous membranes in the manufacture of genomic medicines |
| title_full_unstemmed | Opportunities for isoporous membranes in the manufacture of genomic medicines |
| title_short | Opportunities for isoporous membranes in the manufacture of genomic medicines |
| title_sort | opportunities for isoporous membranes in the manufacture of genomic medicines |
| topic | Isoporous membranes Genomic medicine Bio-separation Ultrafiltration Block copolymer membranes |
| url | http://www.sciencedirect.com/science/article/pii/S2772421223000168 |
| work_keys_str_mv | AT kemeng opportunitiesforisoporousmembranesinthemanufactureofgenomicmedicines AT thomasfjohnson opportunitiesforisoporousmembranesinthemanufactureofgenomicmedicines AT albertoalvarezfernandez opportunitiesforisoporousmembranesinthemanufactureofgenomicmedicines AT stefanguldin opportunitiesforisoporousmembranesinthemanufactureofgenomicmedicines AT danielgbracewell opportunitiesforisoporousmembranesinthemanufactureofgenomicmedicines |