Neurotrophins‐3 plays a vital role in anti‐apoptosis associated with NGF and BDNF regulation in neonatal rats with hypoxic‐ischemic brain injury
Objective To explore the role of Neurotropfin‐3 (NT‐3) in hypoxic‐ischemic encephalopathy (HIE) and determine its relative molecular mechanism. Methods The expression of NT‐3 was determined by qRT‐PCR in the cortex after HIE and immunofluorescence staining was used to detect the distribution of NT‐3...
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Format: | Article |
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Wiley-VCH
2020-01-01
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Series: | Ibrain |
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Online Access: | https://doi.org/10.1002/j.2769-2795.2020.tb00047.x |
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author | Qiu‐Xia Xiao Jun‐Jie Chen Chang‐Le Fang Zhang‐Yu Su Ting‐Hua Wang |
author_facet | Qiu‐Xia Xiao Jun‐Jie Chen Chang‐Le Fang Zhang‐Yu Su Ting‐Hua Wang |
author_sort | Qiu‐Xia Xiao |
collection | DOAJ |
description | Objective To explore the role of Neurotropfin‐3 (NT‐3) in hypoxic‐ischemic encephalopathy (HIE) and determine its relative molecular mechanism. Methods The expression of NT‐3 was determined by qRT‐PCR in the cortex after HIE and immunofluorescence staining was used to detect the distribution of NT‐3. Then, the NT‐3 siRNA was used to validate the effect of NT‐3 for the neuroprotection by the measurement of cell apoptosis after oxygen‐glucose deprivation (OGD). Lastly, the molecular network mechanism was studied by NT‐3 interference experiment combined with bioinformatics technology. Results The expression of NT‐3 was significantly upregulated after HIE, and NT‐3 was co‐localized in the neurons of the cortex. Furthermore, the inhibition of NT‐3 increased neuronal apoptosis after OGD and elicited the down‐regulation of BDNF and NGF. Conclusion NT‐3 is a vital neurotrophic factor that can maintain neonatal survival in cortical neurons, and the underlying mechanism is associated with BDNF and NGF regulation. |
first_indexed | 2024-04-12T15:24:13Z |
format | Article |
id | doaj.art-ca60ad82074747eab3b83f7bc7806ef7 |
institution | Directory Open Access Journal |
issn | 2769-2795 |
language | English |
last_indexed | 2024-04-12T15:24:13Z |
publishDate | 2020-01-01 |
publisher | Wiley-VCH |
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series | Ibrain |
spelling | doaj.art-ca60ad82074747eab3b83f7bc7806ef72022-12-22T03:27:19ZengWiley-VCHIbrain2769-27952020-01-0162121710.1002/j.2769-2795.2020.tb00047.xNeurotrophins‐3 plays a vital role in anti‐apoptosis associated with NGF and BDNF regulation in neonatal rats with hypoxic‐ischemic brain injuryQiu‐Xia Xiao0Jun‐Jie Chen1Chang‐Le Fang2Zhang‐Yu Su3Ting‐Hua Wang4Animal Zoology DepartmentKunming Medical UniversityKunmingYunnanChinaSchool of Anesthesiology, Southwest Medical UniversityLuzhouSichuanChinaSchool of Anesthesiology, Southwest Medical UniversityLuzhouSichuanChinaSchool of Anesthesiology, Southwest Medical UniversityLuzhouSichuanChinaAnimal Zoology DepartmentKunming Medical UniversityKunmingYunnanChinaObjective To explore the role of Neurotropfin‐3 (NT‐3) in hypoxic‐ischemic encephalopathy (HIE) and determine its relative molecular mechanism. Methods The expression of NT‐3 was determined by qRT‐PCR in the cortex after HIE and immunofluorescence staining was used to detect the distribution of NT‐3. Then, the NT‐3 siRNA was used to validate the effect of NT‐3 for the neuroprotection by the measurement of cell apoptosis after oxygen‐glucose deprivation (OGD). Lastly, the molecular network mechanism was studied by NT‐3 interference experiment combined with bioinformatics technology. Results The expression of NT‐3 was significantly upregulated after HIE, and NT‐3 was co‐localized in the neurons of the cortex. Furthermore, the inhibition of NT‐3 increased neuronal apoptosis after OGD and elicited the down‐regulation of BDNF and NGF. Conclusion NT‐3 is a vital neurotrophic factor that can maintain neonatal survival in cortical neurons, and the underlying mechanism is associated with BDNF and NGF regulation.https://doi.org/10.1002/j.2769-2795.2020.tb00047.xHypoxic‐ischemic encephalopathyNeurotrofin‐3Cell apoptosisBio‐informaticsBrain‐derived neurotrophic factorNerve growth factor |
spellingShingle | Qiu‐Xia Xiao Jun‐Jie Chen Chang‐Le Fang Zhang‐Yu Su Ting‐Hua Wang Neurotrophins‐3 plays a vital role in anti‐apoptosis associated with NGF and BDNF regulation in neonatal rats with hypoxic‐ischemic brain injury Ibrain Hypoxic‐ischemic encephalopathy Neurotrofin‐3 Cell apoptosis Bio‐informatics Brain‐derived neurotrophic factor Nerve growth factor |
title | Neurotrophins‐3 plays a vital role in anti‐apoptosis associated with NGF and BDNF regulation in neonatal rats with hypoxic‐ischemic brain injury |
title_full | Neurotrophins‐3 plays a vital role in anti‐apoptosis associated with NGF and BDNF regulation in neonatal rats with hypoxic‐ischemic brain injury |
title_fullStr | Neurotrophins‐3 plays a vital role in anti‐apoptosis associated with NGF and BDNF regulation in neonatal rats with hypoxic‐ischemic brain injury |
title_full_unstemmed | Neurotrophins‐3 plays a vital role in anti‐apoptosis associated with NGF and BDNF regulation in neonatal rats with hypoxic‐ischemic brain injury |
title_short | Neurotrophins‐3 plays a vital role in anti‐apoptosis associated with NGF and BDNF regulation in neonatal rats with hypoxic‐ischemic brain injury |
title_sort | neurotrophins 3 plays a vital role in anti apoptosis associated with ngf and bdnf regulation in neonatal rats with hypoxic ischemic brain injury |
topic | Hypoxic‐ischemic encephalopathy Neurotrofin‐3 Cell apoptosis Bio‐informatics Brain‐derived neurotrophic factor Nerve growth factor |
url | https://doi.org/10.1002/j.2769-2795.2020.tb00047.x |
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