Metabolism and Biodegradation of β-Glucan in vivo
The β-Glucans widely exist in plants and edible fungi, and their diverse bioactivities and good physicochemical properties have been widely reported. In addition, β-glucan intravenous injections (such as lentinan and schizophyllan) have been clinically used as immunomodulators and antitumor polysacc...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2022.889586/full |
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| author | Ziming Zheng Ziming Zheng Wenqi Tang Weipeng Lu Xu Mu Yuxuan Liu Xianglin Pan Xianglin Pan Kaiping Wang Yu Zhang Yu Zhang |
| author_facet | Ziming Zheng Ziming Zheng Wenqi Tang Weipeng Lu Xu Mu Yuxuan Liu Xianglin Pan Xianglin Pan Kaiping Wang Yu Zhang Yu Zhang |
| author_sort | Ziming Zheng |
| collection | DOAJ |
| description | The β-Glucans widely exist in plants and edible fungi, and their diverse bioactivities and good physicochemical properties have been widely reported. In addition, β-glucan intravenous injections (such as lentinan and schizophyllan) have been clinically used as immunomodulators and antitumor polysaccharides. However, the pharmacokinetic studies of β-glucans only stay on the level of plasma concentration and biodistribution in vivo, and little is known about their metabolism and degradation in vivo, which severely limits the further application of β-glucans in the field of medicine and biomaterials. The aim of this paper is to explore the metabolism and degradation process of lentinan (as a representative of β-glucans) in vivo by labeling it with water-soluble fluorescein 5-([4, 6-Dichlorotriazin-2-yl]amino)fluorescein (DTAF). Fluorescently labeled lentinan (FLNT) was intravenously administered to rats at a single dose of 8 mg/kg. The degradation of LNT in blood, liver, kidney, and urine was evaluated by the gel permeation chromatography. Our results showed that although LNT could be degraded in blood, liver, kidney, and urine, there were still some prototypes until excreted in urine due to the incomplete degradation of LNT in each step. To the best of our knowledge, this is the first report to comprehensively study LNT metabolic degradation in rats. These results provide an important reference for further exploration and application of LNT and other β-glucans. |
| first_indexed | 2024-12-12T11:07:36Z |
| format | Article |
| id | doaj.art-ca65a5e9b62a4d2a96a09476e74a1047 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-12-12T11:07:36Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-ca65a5e9b62a4d2a96a09476e74a10472022-12-22T00:26:23ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692022-06-01910.3389/fvets.2022.889586889586Metabolism and Biodegradation of β-Glucan in vivoZiming Zheng0Ziming Zheng1Wenqi Tang2Weipeng Lu3Xu Mu4Yuxuan Liu5Xianglin Pan6Xianglin Pan7Kaiping Wang8Yu Zhang9Yu Zhang10Department of Pharmacy, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaHubei Province Clinical Research Center for Precision Medicine for Critical Illness, Wuhan, ChinaDepartment of Pharmacy, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Pharmacy, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaHubei Key Laboratory of Nature Medicinal Chemistry and Resource Evaluation, Tongji Medical College of Pharmacy, Huazhong University of Science and Technology, Wuhan, ChinaHubei Key Laboratory of Nature Medicinal Chemistry and Resource Evaluation, Tongji Medical College of Pharmacy, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Pharmacy, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Pharmacy, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaHubei Key Laboratory of Nature Medicinal Chemistry and Resource Evaluation, Tongji Medical College of Pharmacy, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Pharmacy, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaHubei Province Clinical Research Center for Precision Medicine for Critical Illness, Wuhan, ChinaThe β-Glucans widely exist in plants and edible fungi, and their diverse bioactivities and good physicochemical properties have been widely reported. In addition, β-glucan intravenous injections (such as lentinan and schizophyllan) have been clinically used as immunomodulators and antitumor polysaccharides. However, the pharmacokinetic studies of β-glucans only stay on the level of plasma concentration and biodistribution in vivo, and little is known about their metabolism and degradation in vivo, which severely limits the further application of β-glucans in the field of medicine and biomaterials. The aim of this paper is to explore the metabolism and degradation process of lentinan (as a representative of β-glucans) in vivo by labeling it with water-soluble fluorescein 5-([4, 6-Dichlorotriazin-2-yl]amino)fluorescein (DTAF). Fluorescently labeled lentinan (FLNT) was intravenously administered to rats at a single dose of 8 mg/kg. The degradation of LNT in blood, liver, kidney, and urine was evaluated by the gel permeation chromatography. Our results showed that although LNT could be degraded in blood, liver, kidney, and urine, there were still some prototypes until excreted in urine due to the incomplete degradation of LNT in each step. To the best of our knowledge, this is the first report to comprehensively study LNT metabolic degradation in rats. These results provide an important reference for further exploration and application of LNT and other β-glucans.https://www.frontiersin.org/articles/10.3389/fvets.2022.889586/fulllentinandegradationmetabolismβ-glucansfluorescent labeling |
| spellingShingle | Ziming Zheng Ziming Zheng Wenqi Tang Weipeng Lu Xu Mu Yuxuan Liu Xianglin Pan Xianglin Pan Kaiping Wang Yu Zhang Yu Zhang Metabolism and Biodegradation of β-Glucan in vivo Frontiers in Veterinary Science lentinan degradation metabolism β-glucans fluorescent labeling |
| title | Metabolism and Biodegradation of β-Glucan in vivo |
| title_full | Metabolism and Biodegradation of β-Glucan in vivo |
| title_fullStr | Metabolism and Biodegradation of β-Glucan in vivo |
| title_full_unstemmed | Metabolism and Biodegradation of β-Glucan in vivo |
| title_short | Metabolism and Biodegradation of β-Glucan in vivo |
| title_sort | metabolism and biodegradation of β glucan in vivo |
| topic | lentinan degradation metabolism β-glucans fluorescent labeling |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2022.889586/full |
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