NADPH performs mediated electron transfer in cyanobacterial-driven bio-photoelectrochemical cells
Summary: Previous studies have shown that live cyanobacteria can produce photocurrent in bio-photoelectrochemical cells (BPECs) that can be exploited for clean renewable energy production. Electron transfer from cyanobacteria to the electrochemical cell was proposed to be facilitated by small molecu...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-01-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004220310890 |
| _version_ | 1818963671587487744 |
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| author | Yaniv Shlosberg Benjamin Eichenbaum Tünde N. Tóth Guy Levin Varda Liveanu Gadi Schuster Noam Adir |
| author_facet | Yaniv Shlosberg Benjamin Eichenbaum Tünde N. Tóth Guy Levin Varda Liveanu Gadi Schuster Noam Adir |
| author_sort | Yaniv Shlosberg |
| collection | DOAJ |
| description | Summary: Previous studies have shown that live cyanobacteria can produce photocurrent in bio-photoelectrochemical cells (BPECs) that can be exploited for clean renewable energy production. Electron transfer from cyanobacteria to the electrochemical cell was proposed to be facilitated by small molecule(s) mediator(s) whose identity (or identities) remain unknown. Here, we elucidate the mechanism of electron transfer in the BPEC by identifying the major electron mediator as NADPH in three cyanobacterial species. We show that an increase in the concentration of NADPH secreted into the external cell medium (ECM) is obtained by both illumination and activation of the BPEC. Elimination of NADPH in the ECM abrogates the photocurrent while addition of exogenous NADP+ significantly increases and prolongs the photocurrent production. NADP+ is thus the first non-toxic, water soluble electron mediator that can functionally link photosynthetic cells to an energy conversion system and may serve to improve the performance of future BPECs. |
| first_indexed | 2024-12-20T12:48:56Z |
| format | Article |
| id | doaj.art-ca66e7c4462a4d19bc8ab815818e242c |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-12-20T12:48:56Z |
| publishDate | 2021-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-ca66e7c4462a4d19bc8ab815818e242c2022-12-21T19:40:14ZengElsevieriScience2589-00422021-01-01241101892NADPH performs mediated electron transfer in cyanobacterial-driven bio-photoelectrochemical cellsYaniv Shlosberg0Benjamin Eichenbaum1Tünde N. Tóth2Guy Levin3Varda Liveanu4Gadi Schuster5Noam Adir6Grand Technion Energy Program, Technion, Haifa 32000, Israel; Schulich Faculty of Chemistry, Technion, Haifa 32000, IsraelFaculty of Biology, Technion, Haifa 32000, IsraelGrand Technion Energy Program, Technion, Haifa 32000, Israel; Schulich Faculty of Chemistry, Technion, Haifa 32000, IsraelFaculty of Biology, Technion, Haifa 32000, IsraelFaculty of Biology, Technion, Haifa 32000, IsraelGrand Technion Energy Program, Technion, Haifa 32000, Israel; Faculty of Biology, Technion, Haifa 32000, Israel; Corresponding authorGrand Technion Energy Program, Technion, Haifa 32000, Israel; Schulich Faculty of Chemistry, Technion, Haifa 32000, Israel; Corresponding authorSummary: Previous studies have shown that live cyanobacteria can produce photocurrent in bio-photoelectrochemical cells (BPECs) that can be exploited for clean renewable energy production. Electron transfer from cyanobacteria to the electrochemical cell was proposed to be facilitated by small molecule(s) mediator(s) whose identity (or identities) remain unknown. Here, we elucidate the mechanism of electron transfer in the BPEC by identifying the major electron mediator as NADPH in three cyanobacterial species. We show that an increase in the concentration of NADPH secreted into the external cell medium (ECM) is obtained by both illumination and activation of the BPEC. Elimination of NADPH in the ECM abrogates the photocurrent while addition of exogenous NADP+ significantly increases and prolongs the photocurrent production. NADP+ is thus the first non-toxic, water soluble electron mediator that can functionally link photosynthetic cells to an energy conversion system and may serve to improve the performance of future BPECs.http://www.sciencedirect.com/science/article/pii/S2589004220310890Bio-ElectrochemistryMicrobiologyBiotechnologyEnergy Engineering |
| spellingShingle | Yaniv Shlosberg Benjamin Eichenbaum Tünde N. Tóth Guy Levin Varda Liveanu Gadi Schuster Noam Adir NADPH performs mediated electron transfer in cyanobacterial-driven bio-photoelectrochemical cells iScience Bio-Electrochemistry Microbiology Biotechnology Energy Engineering |
| title | NADPH performs mediated electron transfer in cyanobacterial-driven bio-photoelectrochemical cells |
| title_full | NADPH performs mediated electron transfer in cyanobacterial-driven bio-photoelectrochemical cells |
| title_fullStr | NADPH performs mediated electron transfer in cyanobacterial-driven bio-photoelectrochemical cells |
| title_full_unstemmed | NADPH performs mediated electron transfer in cyanobacterial-driven bio-photoelectrochemical cells |
| title_short | NADPH performs mediated electron transfer in cyanobacterial-driven bio-photoelectrochemical cells |
| title_sort | nadph performs mediated electron transfer in cyanobacterial driven bio photoelectrochemical cells |
| topic | Bio-Electrochemistry Microbiology Biotechnology Energy Engineering |
| url | http://www.sciencedirect.com/science/article/pii/S2589004220310890 |
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