Development of a colloidal gold immunochromatographic assay strip using monoclonal antibody for rapid detection of porcine deltacoronavirus
Porcine deltacoronavirus (PDCoV) cause diarrhea and dehydration in newborn piglets and has the potential for cross-species transmission. Rapid and early diagnosis is important for preventing and controlling infectious disease. In this study, two monoclonal antibodies (mAbs) were generated, which cou...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1074513/full |
| _version_ | 1797960765831905280 |
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| author | Wei Wang Wei Wang Wei Wang Wei Wang Wei Wang Baochao Fan Baochao Fan Baochao Fan Xuehan Zhang Xuehan Zhang Xuehan Zhang Rongli Guo Rongli Guo Rongli Guo Yongxiang Zhao Yongxiang Zhao Yongxiang Zhao Junming Zhou Junming Zhou Junming Zhou Jinzhu Zhou Jinzhu Zhou Jinzhu Zhou Qi Peng Qi Peng Qi Peng Mingjun Zhu Mingjun Zhu Mingjun Zhu Jizong Li Jizong Li Jizong Li Jizong Li Jizong Li Bin Li Bin Li Bin Li Bin Li Bin Li |
| author_facet | Wei Wang Wei Wang Wei Wang Wei Wang Wei Wang Baochao Fan Baochao Fan Baochao Fan Xuehan Zhang Xuehan Zhang Xuehan Zhang Rongli Guo Rongli Guo Rongli Guo Yongxiang Zhao Yongxiang Zhao Yongxiang Zhao Junming Zhou Junming Zhou Junming Zhou Jinzhu Zhou Jinzhu Zhou Jinzhu Zhou Qi Peng Qi Peng Qi Peng Mingjun Zhu Mingjun Zhu Mingjun Zhu Jizong Li Jizong Li Jizong Li Jizong Li Jizong Li Bin Li Bin Li Bin Li Bin Li Bin Li |
| author_sort | Wei Wang |
| collection | DOAJ |
| description | Porcine deltacoronavirus (PDCoV) cause diarrhea and dehydration in newborn piglets and has the potential for cross-species transmission. Rapid and early diagnosis is important for preventing and controlling infectious disease. In this study, two monoclonal antibodies (mAbs) were generated, which could specifically recognize recombinant PDCoV nucleocapsid (rPDCoV-N) protein. A colloidal gold immunochromatographic assay (GICA) strip using these mAbs was developed to detect PDCoV antigens within 15 min. Results showed that the detection limit of the GICA strip developed in this study was 103 TCID50/ml for the suspension of virus-infected cell culture and 0.125 μg/ml for rPDCoV-N protein, respectively. Besides, the GICA strip showed high specificity with no cross-reactivity with other porcine pathogenic viruses. Three hundred and twenty-five fecal samples were detected for PDCoV using the GICA strip and reverse transcription-quantitative real-time PCR (RT-qPCR). The coincidence rate of the GICA strip and RT-qPCR was 96.9%. The GICA strip had a diagnostic sensitivity of 88.9% and diagnostic specificity of 98.5%. The specific and efficient detection by the strip provides a convenient, rapid, easy to use and valuable diagnostic tool for PDCoV under laboratory and field conditions. |
| first_indexed | 2024-04-11T00:49:24Z |
| format | Article |
| id | doaj.art-ca6a9196e8e546d4afc9109ae21c4fcc |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T00:49:24Z |
| publishDate | 2023-01-01 |
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| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-ca6a9196e8e546d4afc9109ae21c4fcc2023-01-05T10:10:33ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-01-011310.3389/fmicb.2022.10745131074513Development of a colloidal gold immunochromatographic assay strip using monoclonal antibody for rapid detection of porcine deltacoronavirusWei Wang0Wei Wang1Wei Wang2Wei Wang3Wei Wang4Baochao Fan5Baochao Fan6Baochao Fan7Xuehan Zhang8Xuehan Zhang9Xuehan Zhang10Rongli Guo11Rongli Guo12Rongli Guo13Yongxiang Zhao14Yongxiang Zhao15Yongxiang Zhao16Junming Zhou17Junming Zhou18Junming Zhou19Jinzhu Zhou20Jinzhu Zhou21Jinzhu Zhou22Qi Peng23Qi Peng24Qi Peng25Mingjun Zhu26Mingjun Zhu27Mingjun Zhu28Jizong Li29Jizong Li30Jizong Li31Jizong Li32Jizong Li33Bin Li34Bin Li35Bin Li36Bin Li37Bin Li38Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology Ministry of Agriculture and Rural Affairs, Nanjing, ChinaShaoxing Academy of Biomedicine of Zhejiang Sci-Tech University, Shaoxing, ChinaJiangsu Key Laboratory for Food Quality and Safety, State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, ChinaJiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, ChinaKey Laboratory for Prevention and Control of Avian Influenza and Other Major Poultry Diseases, Ministry of Agriculture and Rural Affairs, Guangzhou, ChinaInstitute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology Ministry of Agriculture and Rural Affairs, Nanjing, ChinaJiangsu Key Laboratory for Food Quality and Safety, State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, ChinaJiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, ChinaInstitute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology Ministry of Agriculture and Rural Affairs, Nanjing, ChinaJiangsu Key Laboratory for Food Quality and Safety, State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, ChinaJiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, ChinaInstitute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology Ministry of Agriculture and Rural Affairs, Nanjing, ChinaJiangsu Key Laboratory for Food Quality and Safety, State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, ChinaJiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, ChinaInstitute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology Ministry of Agriculture and Rural Affairs, Nanjing, ChinaJiangsu Key Laboratory for Food Quality and Safety, State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, ChinaJiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, ChinaInstitute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology Ministry of Agriculture and Rural Affairs, Nanjing, ChinaJiangsu Key Laboratory for Food Quality and Safety, State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, ChinaJiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, ChinaInstitute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology Ministry of Agriculture and Rural Affairs, Nanjing, ChinaJiangsu Key Laboratory for Food Quality and Safety, State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, ChinaJiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, ChinaInstitute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology Ministry of Agriculture and Rural Affairs, Nanjing, ChinaJiangsu Key Laboratory for Food Quality and Safety, State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, ChinaJiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, ChinaInstitute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology Ministry of Agriculture and Rural Affairs, Nanjing, ChinaJiangsu Key Laboratory for Food Quality and Safety, State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, ChinaJiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, ChinaInstitute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology Ministry of Agriculture and Rural Affairs, Nanjing, ChinaShaoxing Academy of Biomedicine of Zhejiang Sci-Tech University, Shaoxing, ChinaJiangsu Key Laboratory for Food Quality and Safety, State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, ChinaJiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, ChinaKey Laboratory for Prevention and Control of Avian Influenza and Other Major Poultry Diseases, Ministry of Agriculture and Rural Affairs, Guangzhou, ChinaInstitute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology Ministry of Agriculture and Rural Affairs, Nanjing, ChinaShaoxing Academy of Biomedicine of Zhejiang Sci-Tech University, Shaoxing, ChinaJiangsu Key Laboratory for Food Quality and Safety, State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, ChinaJiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, ChinaKey Laboratory for Prevention and Control of Avian Influenza and Other Major Poultry Diseases, Ministry of Agriculture and Rural Affairs, Guangzhou, ChinaPorcine deltacoronavirus (PDCoV) cause diarrhea and dehydration in newborn piglets and has the potential for cross-species transmission. Rapid and early diagnosis is important for preventing and controlling infectious disease. In this study, two monoclonal antibodies (mAbs) were generated, which could specifically recognize recombinant PDCoV nucleocapsid (rPDCoV-N) protein. A colloidal gold immunochromatographic assay (GICA) strip using these mAbs was developed to detect PDCoV antigens within 15 min. Results showed that the detection limit of the GICA strip developed in this study was 103 TCID50/ml for the suspension of virus-infected cell culture and 0.125 μg/ml for rPDCoV-N protein, respectively. Besides, the GICA strip showed high specificity with no cross-reactivity with other porcine pathogenic viruses. Three hundred and twenty-five fecal samples were detected for PDCoV using the GICA strip and reverse transcription-quantitative real-time PCR (RT-qPCR). The coincidence rate of the GICA strip and RT-qPCR was 96.9%. The GICA strip had a diagnostic sensitivity of 88.9% and diagnostic specificity of 98.5%. The specific and efficient detection by the strip provides a convenient, rapid, easy to use and valuable diagnostic tool for PDCoV under laboratory and field conditions.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1074513/fullporcine deltacoronaviruscolloidal gold immunochromatographic assay (GICA) stripmonoclonal antibodiesreal-time PCRcross-species transmission |
| spellingShingle | Wei Wang Wei Wang Wei Wang Wei Wang Wei Wang Baochao Fan Baochao Fan Baochao Fan Xuehan Zhang Xuehan Zhang Xuehan Zhang Rongli Guo Rongli Guo Rongli Guo Yongxiang Zhao Yongxiang Zhao Yongxiang Zhao Junming Zhou Junming Zhou Junming Zhou Jinzhu Zhou Jinzhu Zhou Jinzhu Zhou Qi Peng Qi Peng Qi Peng Mingjun Zhu Mingjun Zhu Mingjun Zhu Jizong Li Jizong Li Jizong Li Jizong Li Jizong Li Bin Li Bin Li Bin Li Bin Li Bin Li Development of a colloidal gold immunochromatographic assay strip using monoclonal antibody for rapid detection of porcine deltacoronavirus Frontiers in Microbiology porcine deltacoronavirus colloidal gold immunochromatographic assay (GICA) strip monoclonal antibodies real-time PCR cross-species transmission |
| title | Development of a colloidal gold immunochromatographic assay strip using monoclonal antibody for rapid detection of porcine deltacoronavirus |
| title_full | Development of a colloidal gold immunochromatographic assay strip using monoclonal antibody for rapid detection of porcine deltacoronavirus |
| title_fullStr | Development of a colloidal gold immunochromatographic assay strip using monoclonal antibody for rapid detection of porcine deltacoronavirus |
| title_full_unstemmed | Development of a colloidal gold immunochromatographic assay strip using monoclonal antibody for rapid detection of porcine deltacoronavirus |
| title_short | Development of a colloidal gold immunochromatographic assay strip using monoclonal antibody for rapid detection of porcine deltacoronavirus |
| title_sort | development of a colloidal gold immunochromatographic assay strip using monoclonal antibody for rapid detection of porcine deltacoronavirus |
| topic | porcine deltacoronavirus colloidal gold immunochromatographic assay (GICA) strip monoclonal antibodies real-time PCR cross-species transmission |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1074513/full |
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