Genome-Wide Landscape of mRNAs, lncRNAs, and circRNAs during Testicular Development of Yak
Testicular development is a tightly regulated process in mammals. Understanding the molecular mechanisms of yak testicular development will benefit the yak breeding industry. However, the roles of different RNAs, such as mRNA, lncRNA, and circRNA in the testicular development of yak, are still large...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-02-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/5/4420 |
| _version_ | 1797615271538589696 |
|---|---|
| author | Yongfu La Xiaoming Ma Pengjia Bao Min Chu Ping Yan Chunnian Liang Xian Guo |
| author_facet | Yongfu La Xiaoming Ma Pengjia Bao Min Chu Ping Yan Chunnian Liang Xian Guo |
| author_sort | Yongfu La |
| collection | DOAJ |
| description | Testicular development is a tightly regulated process in mammals. Understanding the molecular mechanisms of yak testicular development will benefit the yak breeding industry. However, the roles of different RNAs, such as mRNA, lncRNA, and circRNA in the testicular development of yak, are still largely unclear. In this study, transcriptome analyses were performed on the expression profiles of mRNAs, lncRNAs, and circRNAs in testis tissues of Ashidan yak at different developmental stages, including 6-months-old (M6), 18-months-old (M18), and 30-months-old (M30). A total of 30, 23, and 277 common differentially expressed (DE) mRNAs, lncRNAs, and circRNAs were identified in M6, M18, and M30, respectively. Furthermore, functional enrichment analysis showed that the common DE mRNAs during the entire developmental process were mainly involved in gonadal mesoderm development, cell differentiation, and spermatogenesis processes. Additionally, co-expression network analysis identified the potential lncRNAs related to spermatogenesis, e.g., TCONS_00087394 and TCONS_00012202. Our study provides new information about changes in RNA expression during yak testicular development, which greatly improves our understanding of the molecular mechanisms regulating testicular development in yaks. |
| first_indexed | 2024-03-11T07:23:56Z |
| format | Article |
| id | doaj.art-ca6c6cdef4bd47a28fcbc406bb2f7fe4 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T07:23:56Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-ca6c6cdef4bd47a28fcbc406bb2f7fe42023-11-17T07:48:26ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-02-01245442010.3390/ijms24054420Genome-Wide Landscape of mRNAs, lncRNAs, and circRNAs during Testicular Development of YakYongfu La0Xiaoming Ma1Pengjia Bao2Min Chu3Ping Yan4Chunnian Liang5Xian Guo6Animal Science Department, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, ChinaAnimal Science Department, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, ChinaAnimal Science Department, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, ChinaAnimal Science Department, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, ChinaAnimal Science Department, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, ChinaAnimal Science Department, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, ChinaAnimal Science Department, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, ChinaTesticular development is a tightly regulated process in mammals. Understanding the molecular mechanisms of yak testicular development will benefit the yak breeding industry. However, the roles of different RNAs, such as mRNA, lncRNA, and circRNA in the testicular development of yak, are still largely unclear. In this study, transcriptome analyses were performed on the expression profiles of mRNAs, lncRNAs, and circRNAs in testis tissues of Ashidan yak at different developmental stages, including 6-months-old (M6), 18-months-old (M18), and 30-months-old (M30). A total of 30, 23, and 277 common differentially expressed (DE) mRNAs, lncRNAs, and circRNAs were identified in M6, M18, and M30, respectively. Furthermore, functional enrichment analysis showed that the common DE mRNAs during the entire developmental process were mainly involved in gonadal mesoderm development, cell differentiation, and spermatogenesis processes. Additionally, co-expression network analysis identified the potential lncRNAs related to spermatogenesis, e.g., TCONS_00087394 and TCONS_00012202. Our study provides new information about changes in RNA expression during yak testicular development, which greatly improves our understanding of the molecular mechanisms regulating testicular development in yaks.https://www.mdpi.com/1422-0067/24/5/4420testisyakdevelopmentnon-coding RNAreproduction |
| spellingShingle | Yongfu La Xiaoming Ma Pengjia Bao Min Chu Ping Yan Chunnian Liang Xian Guo Genome-Wide Landscape of mRNAs, lncRNAs, and circRNAs during Testicular Development of Yak International Journal of Molecular Sciences testis yak development non-coding RNA reproduction |
| title | Genome-Wide Landscape of mRNAs, lncRNAs, and circRNAs during Testicular Development of Yak |
| title_full | Genome-Wide Landscape of mRNAs, lncRNAs, and circRNAs during Testicular Development of Yak |
| title_fullStr | Genome-Wide Landscape of mRNAs, lncRNAs, and circRNAs during Testicular Development of Yak |
| title_full_unstemmed | Genome-Wide Landscape of mRNAs, lncRNAs, and circRNAs during Testicular Development of Yak |
| title_short | Genome-Wide Landscape of mRNAs, lncRNAs, and circRNAs during Testicular Development of Yak |
| title_sort | genome wide landscape of mrnas lncrnas and circrnas during testicular development of yak |
| topic | testis yak development non-coding RNA reproduction |
| url | https://www.mdpi.com/1422-0067/24/5/4420 |
| work_keys_str_mv | AT yongfula genomewidelandscapeofmrnaslncrnasandcircrnasduringtesticulardevelopmentofyak AT xiaomingma genomewidelandscapeofmrnaslncrnasandcircrnasduringtesticulardevelopmentofyak AT pengjiabao genomewidelandscapeofmrnaslncrnasandcircrnasduringtesticulardevelopmentofyak AT minchu genomewidelandscapeofmrnaslncrnasandcircrnasduringtesticulardevelopmentofyak AT pingyan genomewidelandscapeofmrnaslncrnasandcircrnasduringtesticulardevelopmentofyak AT chunnianliang genomewidelandscapeofmrnaslncrnasandcircrnasduringtesticulardevelopmentofyak AT xianguo genomewidelandscapeofmrnaslncrnasandcircrnasduringtesticulardevelopmentofyak |