Bioadhesion on Textured Interfaces in the Human Oral Cavity—An In Situ Study
Extensive biofilm formation on materials used in restorative dentistry is a common reason for their failure and the development of oral diseases like peri-implantitis or secondary caries. Therefore, novel materials and strategies that result in reduced biofouling capacities are urgently sought. Prev...
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MDPI AG
2022-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/3/1157 |
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| author | Ralf Helbig Matthias Hannig Sabine Basche Janis Ortgies Sebastian Killge Christian Hannig Torsten Sterzenbach |
| author_facet | Ralf Helbig Matthias Hannig Sabine Basche Janis Ortgies Sebastian Killge Christian Hannig Torsten Sterzenbach |
| author_sort | Ralf Helbig |
| collection | DOAJ |
| description | Extensive biofilm formation on materials used in restorative dentistry is a common reason for their failure and the development of oral diseases like peri-implantitis or secondary caries. Therefore, novel materials and strategies that result in reduced biofouling capacities are urgently sought. Previous research suggests that surface structures in the range of bacterial cell sizes seem to be a promising approach to modulate bacterial adhesion and biofilm formation. Here we investigated bioadhesion within the oral cavity on a low surface energy material (perfluorpolyether) with different texture types (line-, hole-, pillar-like), feature sizes in a range from 0.7–4.5 µm and graded distances (0.7–130.5 µm). As a model system, the materials were fixed on splints and exposed to the oral cavity. We analyzed the enzymatic activity of amylase and lysozyme, pellicle formation, and bacterial colonization after 8 h intraoral exposure. In opposite to in vitro experiments, these in situ experiments revealed no clear signs of altered bacterial surface colonization regarding structure dimensions and texture types compared to unstructured substrates or natural enamel. In part, there seemed to be a decreasing trend of adherent cells with increasing periodicities and structure sizes, but this pattern was weak and irregular. Pellicle formation took place on all substrates in an unaltered manner. However, pellicle formation was most pronounced within recessed areas thereby partially masking the three-dimensional character of the surfaces. As the natural pellicle layer is obviously the most dominant prerequisite for bacterial adhesion, colonization in the oral environment cannot be easily controlled by structural means. |
| first_indexed | 2024-03-09T23:50:00Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T23:50:00Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-ca6d64b2905746b991dd593382e0f0ca2023-11-23T16:35:55ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-01-01233115710.3390/ijms23031157Bioadhesion on Textured Interfaces in the Human Oral Cavity—An In Situ StudyRalf Helbig0Matthias Hannig1Sabine Basche2Janis Ortgies3Sebastian Killge4Christian Hannig5Torsten Sterzenbach6Max Bergmann Center of Biomaterials, Leibniz-Institut für Polymerforschung, Hohe Straße 6, 01069 Dresden, GermanyClinic of Operative Dentistry, Periodontology and Preventive Dentistry, University Hospital, Saarland University, 66421 Homburg, GermanyClinic of Operative and Pediatric Density, Medical Faculty Carl Gustav Carus, Technische Universität Dresden, Fetscherstraße 74, 01307 Dresden, GermanyClinic of Operative Dentistry, Periodontology and Preventive Dentistry, University Hospital, Saarland University, 66421 Homburg, GermanyInstitute of Semiconductor and Microsystems, Chair of Nanoelectronics, Technische Universität Dresden, 01609 Dresden, GermanyClinic of Operative and Pediatric Density, Medical Faculty Carl Gustav Carus, Technische Universität Dresden, Fetscherstraße 74, 01307 Dresden, GermanyClinic of Operative and Pediatric Density, Medical Faculty Carl Gustav Carus, Technische Universität Dresden, Fetscherstraße 74, 01307 Dresden, GermanyExtensive biofilm formation on materials used in restorative dentistry is a common reason for their failure and the development of oral diseases like peri-implantitis or secondary caries. Therefore, novel materials and strategies that result in reduced biofouling capacities are urgently sought. Previous research suggests that surface structures in the range of bacterial cell sizes seem to be a promising approach to modulate bacterial adhesion and biofilm formation. Here we investigated bioadhesion within the oral cavity on a low surface energy material (perfluorpolyether) with different texture types (line-, hole-, pillar-like), feature sizes in a range from 0.7–4.5 µm and graded distances (0.7–130.5 µm). As a model system, the materials were fixed on splints and exposed to the oral cavity. We analyzed the enzymatic activity of amylase and lysozyme, pellicle formation, and bacterial colonization after 8 h intraoral exposure. In opposite to in vitro experiments, these in situ experiments revealed no clear signs of altered bacterial surface colonization regarding structure dimensions and texture types compared to unstructured substrates or natural enamel. In part, there seemed to be a decreasing trend of adherent cells with increasing periodicities and structure sizes, but this pattern was weak and irregular. Pellicle formation took place on all substrates in an unaltered manner. However, pellicle formation was most pronounced within recessed areas thereby partially masking the three-dimensional character of the surfaces. As the natural pellicle layer is obviously the most dominant prerequisite for bacterial adhesion, colonization in the oral environment cannot be easily controlled by structural means.https://www.mdpi.com/1422-0067/23/3/1157textured surfacesbioadhesionoralpelliclemicrobiotarestorative dentistry |
| spellingShingle | Ralf Helbig Matthias Hannig Sabine Basche Janis Ortgies Sebastian Killge Christian Hannig Torsten Sterzenbach Bioadhesion on Textured Interfaces in the Human Oral Cavity—An In Situ Study International Journal of Molecular Sciences textured surfaces bioadhesion oral pellicle microbiota restorative dentistry |
| title | Bioadhesion on Textured Interfaces in the Human Oral Cavity—An In Situ Study |
| title_full | Bioadhesion on Textured Interfaces in the Human Oral Cavity—An In Situ Study |
| title_fullStr | Bioadhesion on Textured Interfaces in the Human Oral Cavity—An In Situ Study |
| title_full_unstemmed | Bioadhesion on Textured Interfaces in the Human Oral Cavity—An In Situ Study |
| title_short | Bioadhesion on Textured Interfaces in the Human Oral Cavity—An In Situ Study |
| title_sort | bioadhesion on textured interfaces in the human oral cavity an in situ study |
| topic | textured surfaces bioadhesion oral pellicle microbiota restorative dentistry |
| url | https://www.mdpi.com/1422-0067/23/3/1157 |
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