A capsid-encoded PPxY-motif facilitates adenovirus entry.
Viruses use cellular machinery to enter and infect cells. In this study we address the cell entry mechanisms of nonenveloped adenoviruses (Ads). We show that protein VI, an internal capsid protein, is rapidly exposed after cell surface attachment and internalization and remains partially associated...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2010-03-01
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| Series: | PLoS Pathogens |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20333243/pdf/?tool=EBI |
| _version_ | 1818839371318558720 |
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| author | Harald Wodrich Daniel Henaff Baptist Jammart Carolina Segura-Morales Sigrid Seelmeir Olivier Coux Zsolt Ruzsics Christopher M Wiethoff Eric J Kremer |
| author_facet | Harald Wodrich Daniel Henaff Baptist Jammart Carolina Segura-Morales Sigrid Seelmeir Olivier Coux Zsolt Ruzsics Christopher M Wiethoff Eric J Kremer |
| author_sort | Harald Wodrich |
| collection | DOAJ |
| description | Viruses use cellular machinery to enter and infect cells. In this study we address the cell entry mechanisms of nonenveloped adenoviruses (Ads). We show that protein VI, an internal capsid protein, is rapidly exposed after cell surface attachment and internalization and remains partially associated with the capsid during intracellular transport. We found that a PPxY motif within protein VI recruits Nedd4 E3 ubiquitin ligases to bind and ubiquitylate protein VI. We further show that this PPxY motif is involved in rapid, microtubule-dependent intracellular movement of protein VI. Ads with a mutated PPxY motif can efficiently escape endosomes but are defective in microtubule-dependent trafficking toward the nucleus. Likewise, depletion of Nedd4 ligases attenuates nuclear accumulation of incoming Ad particles and infection. Our data provide the first evidence that virus-encoded PPxY motifs are required during virus entry, which may be of significance for several other pathogens. |
| first_indexed | 2024-12-19T03:53:14Z |
| format | Article |
| id | doaj.art-ca71ac1e8ace4d4aa1b5123ec554c75a |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-12-19T03:53:14Z |
| publishDate | 2010-03-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-ca71ac1e8ace4d4aa1b5123ec554c75a2022-12-21T20:36:53ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742010-03-0163e100080810.1371/journal.ppat.1000808A capsid-encoded PPxY-motif facilitates adenovirus entry.Harald WodrichDaniel HenaffBaptist JammartCarolina Segura-MoralesSigrid SeelmeirOlivier CouxZsolt RuzsicsChristopher M WiethoffEric J KremerViruses use cellular machinery to enter and infect cells. In this study we address the cell entry mechanisms of nonenveloped adenoviruses (Ads). We show that protein VI, an internal capsid protein, is rapidly exposed after cell surface attachment and internalization and remains partially associated with the capsid during intracellular transport. We found that a PPxY motif within protein VI recruits Nedd4 E3 ubiquitin ligases to bind and ubiquitylate protein VI. We further show that this PPxY motif is involved in rapid, microtubule-dependent intracellular movement of protein VI. Ads with a mutated PPxY motif can efficiently escape endosomes but are defective in microtubule-dependent trafficking toward the nucleus. Likewise, depletion of Nedd4 ligases attenuates nuclear accumulation of incoming Ad particles and infection. Our data provide the first evidence that virus-encoded PPxY motifs are required during virus entry, which may be of significance for several other pathogens.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20333243/pdf/?tool=EBI |
| spellingShingle | Harald Wodrich Daniel Henaff Baptist Jammart Carolina Segura-Morales Sigrid Seelmeir Olivier Coux Zsolt Ruzsics Christopher M Wiethoff Eric J Kremer A capsid-encoded PPxY-motif facilitates adenovirus entry. PLoS Pathogens |
| title | A capsid-encoded PPxY-motif facilitates adenovirus entry. |
| title_full | A capsid-encoded PPxY-motif facilitates adenovirus entry. |
| title_fullStr | A capsid-encoded PPxY-motif facilitates adenovirus entry. |
| title_full_unstemmed | A capsid-encoded PPxY-motif facilitates adenovirus entry. |
| title_short | A capsid-encoded PPxY-motif facilitates adenovirus entry. |
| title_sort | capsid encoded ppxy motif facilitates adenovirus entry |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20333243/pdf/?tool=EBI |
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