Brain and plasma amino acid concentration in infant rats prenatally exposed to valproic acid
Abstract Autism spectrum disorder is associated with alterations in GABAergic and glutamatergic neurotransmission. Here, we aimed to determine the concentration of GABA, glutamate, glutamine, aspartate, taurine, and glycine in brain tissue and plasma of rats prenatally exposed to valproic acid (VPA)...
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Academia Brasileira de Ciências
2021-03-01
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| Series: | Anais da Academia Brasileira de Ciências |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000300806&tlng=en |
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| author | ÁNGEL ALBERTO PUIG-LAGUNES LUISA ROCHA CONSUELO MORGADO-VALLE LUIS BELTRÁN-PARRAZAL MARÍA-LEONOR LÓPEZ-MERAZ |
| author_facet | ÁNGEL ALBERTO PUIG-LAGUNES LUISA ROCHA CONSUELO MORGADO-VALLE LUIS BELTRÁN-PARRAZAL MARÍA-LEONOR LÓPEZ-MERAZ |
| author_sort | ÁNGEL ALBERTO PUIG-LAGUNES |
| collection | DOAJ |
| description | Abstract Autism spectrum disorder is associated with alterations in GABAergic and glutamatergic neurotransmission. Here, we aimed to determine the concentration of GABA, glutamate, glutamine, aspartate, taurine, and glycine in brain tissue and plasma of rats prenatally exposed to valproic acid (VPA), a well-characterized experimental model of autism. Pregnant rats were injected with VPA (600mg/Kg) during the twelfth-embryonic-day. Control rats were injected with saline. On the fourteen-postnatal-day, rats from both groups (males and females) were anesthetized, euthanized by decapitation and their brain dissected out. The frontal cortex, hippocampus, amygdala, brain stem and cerebellum were dissected and homogenized. Homogenates were centrifuged and supernatants were used to quantify amino acid concentrations by HPLC coupled with fluorometric detection. Blood samples were obtained by a cardiac puncture; plasma was separated and deproteinized to quantify amino acid concentration by HPLC. We found that, in VPA rats, glutamate and glutamine concentrations were increased in hippocampus and glycine concentration was increased in cortex. We did not find changes in other regions or in plasma amino acid concentration in the VPA group with respect to control group. Our results suggest that VPA exposure in utero may impair inhibitory and excitatory amino acid transmission in the infant brain. |
| first_indexed | 2024-12-20T09:23:46Z |
| format | Article |
| id | doaj.art-ca734aa793414b289d1a00d8ba36e582 |
| institution | Directory Open Access Journal |
| issn | 1678-2690 |
| language | English |
| last_indexed | 2024-12-20T09:23:46Z |
| publishDate | 2021-03-01 |
| publisher | Academia Brasileira de Ciências |
| record_format | Article |
| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj.art-ca734aa793414b289d1a00d8ba36e5822022-12-21T19:45:14ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902021-03-0193210.1590/0001-3765202120190861Brain and plasma amino acid concentration in infant rats prenatally exposed to valproic acidÁNGEL ALBERTO PUIG-LAGUNEShttps://orcid.org/0000-0003-0177-3921LUISA ROCHAhttps://orcid.org/0000-0003-4495-9427CONSUELO MORGADO-VALLEhttps://orcid.org/0000-0003-0158-7096LUIS BELTRÁN-PARRAZALhttps://orcid.org/0000-0002-4765-9051MARÍA-LEONOR LÓPEZ-MERAZhttps://orcid.org/0000-0003-0179-989XAbstract Autism spectrum disorder is associated with alterations in GABAergic and glutamatergic neurotransmission. Here, we aimed to determine the concentration of GABA, glutamate, glutamine, aspartate, taurine, and glycine in brain tissue and plasma of rats prenatally exposed to valproic acid (VPA), a well-characterized experimental model of autism. Pregnant rats were injected with VPA (600mg/Kg) during the twelfth-embryonic-day. Control rats were injected with saline. On the fourteen-postnatal-day, rats from both groups (males and females) were anesthetized, euthanized by decapitation and their brain dissected out. The frontal cortex, hippocampus, amygdala, brain stem and cerebellum were dissected and homogenized. Homogenates were centrifuged and supernatants were used to quantify amino acid concentrations by HPLC coupled with fluorometric detection. Blood samples were obtained by a cardiac puncture; plasma was separated and deproteinized to quantify amino acid concentration by HPLC. We found that, in VPA rats, glutamate and glutamine concentrations were increased in hippocampus and glycine concentration was increased in cortex. We did not find changes in other regions or in plasma amino acid concentration in the VPA group with respect to control group. Our results suggest that VPA exposure in utero may impair inhibitory and excitatory amino acid transmission in the infant brain.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000300806&tlng=enAmino acidautismdeveloping brainvalproic acid |
| spellingShingle | ÁNGEL ALBERTO PUIG-LAGUNES LUISA ROCHA CONSUELO MORGADO-VALLE LUIS BELTRÁN-PARRAZAL MARÍA-LEONOR LÓPEZ-MERAZ Brain and plasma amino acid concentration in infant rats prenatally exposed to valproic acid Anais da Academia Brasileira de Ciências Amino acid autism developing brain valproic acid |
| title | Brain and plasma amino acid concentration in infant rats prenatally exposed to valproic acid |
| title_full | Brain and plasma amino acid concentration in infant rats prenatally exposed to valproic acid |
| title_fullStr | Brain and plasma amino acid concentration in infant rats prenatally exposed to valproic acid |
| title_full_unstemmed | Brain and plasma amino acid concentration in infant rats prenatally exposed to valproic acid |
| title_short | Brain and plasma amino acid concentration in infant rats prenatally exposed to valproic acid |
| title_sort | brain and plasma amino acid concentration in infant rats prenatally exposed to valproic acid |
| topic | Amino acid autism developing brain valproic acid |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652021000300806&tlng=en |
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