Identification of Daphnane Diterpenoids from <i>Wikstroemia indica</i> Using Liquid Chromatography with Tandem Mass Spectrometry
Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) has emerged as a powerful tool for the rapid identification of compounds within natural resources. Daphnane diterpenoids, a class of natural compounds predominantly found in plants belonging to the Thymelaeaceae and Euphorbiaceae...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-10-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/12/20/3620 |
| _version_ | 1797572489193193472 |
|---|---|
| author | Mi Zhang Kouharu Otsuki Reo Takahashi Takashi Kikuchi Di Zhou Ning Li Wei Li |
| author_facet | Mi Zhang Kouharu Otsuki Reo Takahashi Takashi Kikuchi Di Zhou Ning Li Wei Li |
| author_sort | Mi Zhang |
| collection | DOAJ |
| description | Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) has emerged as a powerful tool for the rapid identification of compounds within natural resources. Daphnane diterpenoids, a class of natural compounds predominantly found in plants belonging to the Thymelaeaceae and Euphorbiaceae families, have attracted much attention due to their remarkable anticancer and anti-HIV activities. In the present study, the presence of daphnane diterpenoids in <i>Wikstroemia indica</i>, a plant belonging to the Thymelaeaceae family, was investigated by LC-MS/MS analysis. As a result, 21 daphnane diterpenoids (<b>1</b>–<b>21</b>) in the stems of <i>W. indica</i> were detected. Among these, six major compounds (<b>12</b>, <b>15</b>, <b>17</b>, <b>18</b>, <b>20</b>, and <b>21</b>) were isolated and their structures were unequivocally identified through a comprehensive analysis of the MS and NMR data. For the minor compounds (<b>1</b>–<b>11</b>, <b>13</b>, <b>14</b>, <b>16</b>, and <b>19</b>), their structures were elucidated by in-depth MS/MS fragmentation analysis. This study represents the first disclosure of structurally diverse daphnane diterpenoids in <i>W. indica</i>, significantly contributing to our understanding of bioactive diterpenoids in plants within the Thymelaeaceae family. |
| first_indexed | 2024-03-10T20:56:59Z |
| format | Article |
| id | doaj.art-ca7590669f5a477fbe8e42da2bee61bb |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T20:56:59Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-ca7590669f5a477fbe8e42da2bee61bb2023-11-19T17:49:37ZengMDPI AGPlants2223-77472023-10-011220362010.3390/plants12203620Identification of Daphnane Diterpenoids from <i>Wikstroemia indica</i> Using Liquid Chromatography with Tandem Mass SpectrometryMi Zhang0Kouharu Otsuki1Reo Takahashi2Takashi Kikuchi3Di Zhou4Ning Li5Wei Li6Faculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi 274-8510, Chiba, JapanFaculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi 274-8510, Chiba, JapanFaculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi 274-8510, Chiba, JapanFaculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi 274-8510, Chiba, JapanKey Laboratory of Innovative Traditional Chinese Medicine for Major Chronic Diseases of Liaoning Province, Key Laboratory for TCM Material Basis Study and Innovative Drug Development of Shenyang City, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, ChinaKey Laboratory of Innovative Traditional Chinese Medicine for Major Chronic Diseases of Liaoning Province, Key Laboratory for TCM Material Basis Study and Innovative Drug Development of Shenyang City, School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, ChinaFaculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi 274-8510, Chiba, JapanLiquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) has emerged as a powerful tool for the rapid identification of compounds within natural resources. Daphnane diterpenoids, a class of natural compounds predominantly found in plants belonging to the Thymelaeaceae and Euphorbiaceae families, have attracted much attention due to their remarkable anticancer and anti-HIV activities. In the present study, the presence of daphnane diterpenoids in <i>Wikstroemia indica</i>, a plant belonging to the Thymelaeaceae family, was investigated by LC-MS/MS analysis. As a result, 21 daphnane diterpenoids (<b>1</b>–<b>21</b>) in the stems of <i>W. indica</i> were detected. Among these, six major compounds (<b>12</b>, <b>15</b>, <b>17</b>, <b>18</b>, <b>20</b>, and <b>21</b>) were isolated and their structures were unequivocally identified through a comprehensive analysis of the MS and NMR data. For the minor compounds (<b>1</b>–<b>11</b>, <b>13</b>, <b>14</b>, <b>16</b>, and <b>19</b>), their structures were elucidated by in-depth MS/MS fragmentation analysis. This study represents the first disclosure of structurally diverse daphnane diterpenoids in <i>W. indica</i>, significantly contributing to our understanding of bioactive diterpenoids in plants within the Thymelaeaceae family.https://www.mdpi.com/2223-7747/12/20/3620daphnane diterpenoidsLC-MS/MS<i>Wikstroemia indica</i>MS/MS fragmentation |
| spellingShingle | Mi Zhang Kouharu Otsuki Reo Takahashi Takashi Kikuchi Di Zhou Ning Li Wei Li Identification of Daphnane Diterpenoids from <i>Wikstroemia indica</i> Using Liquid Chromatography with Tandem Mass Spectrometry Plants daphnane diterpenoids LC-MS/MS <i>Wikstroemia indica</i> MS/MS fragmentation |
| title | Identification of Daphnane Diterpenoids from <i>Wikstroemia indica</i> Using Liquid Chromatography with Tandem Mass Spectrometry |
| title_full | Identification of Daphnane Diterpenoids from <i>Wikstroemia indica</i> Using Liquid Chromatography with Tandem Mass Spectrometry |
| title_fullStr | Identification of Daphnane Diterpenoids from <i>Wikstroemia indica</i> Using Liquid Chromatography with Tandem Mass Spectrometry |
| title_full_unstemmed | Identification of Daphnane Diterpenoids from <i>Wikstroemia indica</i> Using Liquid Chromatography with Tandem Mass Spectrometry |
| title_short | Identification of Daphnane Diterpenoids from <i>Wikstroemia indica</i> Using Liquid Chromatography with Tandem Mass Spectrometry |
| title_sort | identification of daphnane diterpenoids from i wikstroemia indica i using liquid chromatography with tandem mass spectrometry |
| topic | daphnane diterpenoids LC-MS/MS <i>Wikstroemia indica</i> MS/MS fragmentation |
| url | https://www.mdpi.com/2223-7747/12/20/3620 |
| work_keys_str_mv | AT mizhang identificationofdaphnanediterpenoidsfromiwikstroemiaindicaiusingliquidchromatographywithtandemmassspectrometry AT kouharuotsuki identificationofdaphnanediterpenoidsfromiwikstroemiaindicaiusingliquidchromatographywithtandemmassspectrometry AT reotakahashi identificationofdaphnanediterpenoidsfromiwikstroemiaindicaiusingliquidchromatographywithtandemmassspectrometry AT takashikikuchi identificationofdaphnanediterpenoidsfromiwikstroemiaindicaiusingliquidchromatographywithtandemmassspectrometry AT dizhou identificationofdaphnanediterpenoidsfromiwikstroemiaindicaiusingliquidchromatographywithtandemmassspectrometry AT ningli identificationofdaphnanediterpenoidsfromiwikstroemiaindicaiusingliquidchromatographywithtandemmassspectrometry AT weili identificationofdaphnanediterpenoidsfromiwikstroemiaindicaiusingliquidchromatographywithtandemmassspectrometry |