Percutaneous ethanol injection enhanced the efficacy of radiofrequency ablation in the treatment of HCC: an insight into the mechanism of ethanol action
Purpose To discuss the possible reasons why percutaneous intratumoral ethanol injection (PEI) combined with radiofrequency ablation (RFA) to treat hepatocellular carcinoma (HCC) reduced the recurrence and metastasis compared with RFA alone. Materials and methods Forty VX2 tumor-bearing rabbits were...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-01-01
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| Series: | International Journal of Hyperthermia |
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| Online Access: | http://dx.doi.org/10.1080/02656736.2021.1977857 |
| _version_ | 1819319845925158912 |
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| author | Yang Tan Xi Ding Haiyi Long Jieyi Ye Tongyi Huang Yuan Lin Mingde LV Xiaoyan Xie Guangliang Huang |
| author_facet | Yang Tan Xi Ding Haiyi Long Jieyi Ye Tongyi Huang Yuan Lin Mingde LV Xiaoyan Xie Guangliang Huang |
| author_sort | Yang Tan |
| collection | DOAJ |
| description | Purpose To discuss the possible reasons why percutaneous intratumoral ethanol injection (PEI) combined with radiofrequency ablation (RFA) to treat hepatocellular carcinoma (HCC) reduced the recurrence and metastasis compared with RFA alone. Materials and methods Forty VX2 tumor-bearing rabbits were randomly divided into four groups (n = 10): the PEI, RFA, PEI-RFA, and control groups. Five rabbits from each group were sacrificed on the 3rd and 7th days after ablation. The number of metastatic tumors in the lung was counted. The ablation volume was measured, and residual tumor specimens were prepared for hematoxylin and eosin staining and caspase-3, Ki-67, and VEGF immunohistochemical staining. Results The volume of ablation in the PEI-RFA group was significantly larger than that in the RFA and PEI groups (p < 0.05). However, no significant differences in the number of lung metastases after ablation were observed among the groups (p > 0.05). The number of microthrombi in the PEI-RFA group was greater than that in the control and RFA groups (p < 0.001 and p < 0.05). The Ki-67 labeling index (LI) and H-score of VEGF in the PEI-RFA group were lower than those in the RFA group, while the H-score of caspase-3 was higher than that in the RFA group on the 7th day after ablation (p < 0.05). Conclusion PEI occluded blood vessels by inducing microthrombi formation, and thereby reducing heat dissipation and increasing the effect of RFA. More importantly, in comparison with an incomplete RFA, PEI-RFA inhibited the increase in the Ki-67 and VEGF expression levels and the decrease in the caspase-3 expression level to happen at some extent and therefore improved the prognosis. |
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| issn | 0265-6736 1464-5157 |
| language | English |
| last_indexed | 2024-12-24T11:10:10Z |
| publishDate | 2021-01-01 |
| publisher | Taylor & Francis Group |
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| series | International Journal of Hyperthermia |
| spelling | doaj.art-ca78d33131d44c1dbb6fe5c05e0ba4cb2022-12-21T16:58:32ZengTaylor & Francis GroupInternational Journal of Hyperthermia0265-67361464-51572021-01-013811394140010.1080/02656736.2021.19778571977857Percutaneous ethanol injection enhanced the efficacy of radiofrequency ablation in the treatment of HCC: an insight into the mechanism of ethanol actionYang Tan0Xi Ding1Haiyi Long2Jieyi Ye3Tongyi Huang4Yuan Lin5Mingde LV6Xiaoyan Xie7Guangliang Huang8Department of Medical Ultrasonics, Division of Interventional Ultrasound, Institute of Diagnostic and Interventional Ultrasound, The First Affiliated Hospital of Sun Yat-sen UniversityDepartment of Medical Ultrasonics, The First Affiliated Hospital, Zhejiang University School of MedicineDepartment of Medical Ultrasonics, Division of Interventional Ultrasound, Institute of Diagnostic and Interventional Ultrasound, The First Affiliated Hospital of Sun Yat-sen UniversityDivision of Interventional Ultrasound, Department of Medical Ultrasonics, Foshan First Municipal People’s Hospital, The Affiliated Foshan Hospital of Sun Yat-sen UniversityDepartment of Medical Ultrasonics, Division of Interventional Ultrasound, Institute of Diagnostic and Interventional Ultrasound, The First Affiliated Hospital of Sun Yat-sen UniversityDepartment of Pathology, The First Affiliated Hospital of Sun Yat-sen UniversityDepartment of Medical Ultrasonics, Division of Interventional Ultrasound, Institute of Diagnostic and Interventional Ultrasound, The First Affiliated Hospital of Sun Yat-sen UniversityDepartment of Medical Ultrasonics, Division of Interventional Ultrasound, Institute of Diagnostic and Interventional Ultrasound, The First Affiliated Hospital of Sun Yat-sen UniversityDepartment of Medical Ultrasonics, Division of Interventional Ultrasound, Institute of Diagnostic and Interventional Ultrasound, The First Affiliated Hospital of Sun Yat-sen UniversityPurpose To discuss the possible reasons why percutaneous intratumoral ethanol injection (PEI) combined with radiofrequency ablation (RFA) to treat hepatocellular carcinoma (HCC) reduced the recurrence and metastasis compared with RFA alone. Materials and methods Forty VX2 tumor-bearing rabbits were randomly divided into four groups (n = 10): the PEI, RFA, PEI-RFA, and control groups. Five rabbits from each group were sacrificed on the 3rd and 7th days after ablation. The number of metastatic tumors in the lung was counted. The ablation volume was measured, and residual tumor specimens were prepared for hematoxylin and eosin staining and caspase-3, Ki-67, and VEGF immunohistochemical staining. Results The volume of ablation in the PEI-RFA group was significantly larger than that in the RFA and PEI groups (p < 0.05). However, no significant differences in the number of lung metastases after ablation were observed among the groups (p > 0.05). The number of microthrombi in the PEI-RFA group was greater than that in the control and RFA groups (p < 0.001 and p < 0.05). The Ki-67 labeling index (LI) and H-score of VEGF in the PEI-RFA group were lower than those in the RFA group, while the H-score of caspase-3 was higher than that in the RFA group on the 7th day after ablation (p < 0.05). Conclusion PEI occluded blood vessels by inducing microthrombi formation, and thereby reducing heat dissipation and increasing the effect of RFA. More importantly, in comparison with an incomplete RFA, PEI-RFA inhibited the increase in the Ki-67 and VEGF expression levels and the decrease in the caspase-3 expression level to happen at some extent and therefore improved the prognosis.http://dx.doi.org/10.1080/02656736.2021.1977857hepatocellular carcinomaradiofrequency ablationpercutaneous ethanol injectionmicrothrombirecurrence |
| spellingShingle | Yang Tan Xi Ding Haiyi Long Jieyi Ye Tongyi Huang Yuan Lin Mingde LV Xiaoyan Xie Guangliang Huang Percutaneous ethanol injection enhanced the efficacy of radiofrequency ablation in the treatment of HCC: an insight into the mechanism of ethanol action International Journal of Hyperthermia hepatocellular carcinoma radiofrequency ablation percutaneous ethanol injection microthrombi recurrence |
| title | Percutaneous ethanol injection enhanced the efficacy of radiofrequency ablation in the treatment of HCC: an insight into the mechanism of ethanol action |
| title_full | Percutaneous ethanol injection enhanced the efficacy of radiofrequency ablation in the treatment of HCC: an insight into the mechanism of ethanol action |
| title_fullStr | Percutaneous ethanol injection enhanced the efficacy of radiofrequency ablation in the treatment of HCC: an insight into the mechanism of ethanol action |
| title_full_unstemmed | Percutaneous ethanol injection enhanced the efficacy of radiofrequency ablation in the treatment of HCC: an insight into the mechanism of ethanol action |
| title_short | Percutaneous ethanol injection enhanced the efficacy of radiofrequency ablation in the treatment of HCC: an insight into the mechanism of ethanol action |
| title_sort | percutaneous ethanol injection enhanced the efficacy of radiofrequency ablation in the treatment of hcc an insight into the mechanism of ethanol action |
| topic | hepatocellular carcinoma radiofrequency ablation percutaneous ethanol injection microthrombi recurrence |
| url | http://dx.doi.org/10.1080/02656736.2021.1977857 |
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