Evolutionarily-Related <i>Helicobacter pylori</i> Genotypes and Gastric Intraepithelial Neoplasia in a High-Risk Area of Northern Italy
<i>Helicobacter pylori</i> (<i>Hp</i>) is the major recognized risk factor for non-cardia gastric cancer (GC), but only a fraction of infected subjects develop GC, thus GC risk might reflect other genetic/environmental cofactors and/or differences in virulence among infectiou...
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| Format: | Article |
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MDPI AG
2020-02-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/3/324 |
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| author | Sonia Toracchio Rosario Alberto Caruso Silvia Perconti Luciana Rigoli Enrico Betri Matteo Neri Fabio Verginelli Renato Mariani-Costantini |
| author_facet | Sonia Toracchio Rosario Alberto Caruso Silvia Perconti Luciana Rigoli Enrico Betri Matteo Neri Fabio Verginelli Renato Mariani-Costantini |
| author_sort | Sonia Toracchio |
| collection | DOAJ |
| description | <i>Helicobacter pylori</i> (<i>Hp</i>) is the major recognized risk factor for non-cardia gastric cancer (GC), but only a fraction of infected subjects develop GC, thus GC risk might reflect other genetic/environmental cofactors and/or differences in virulence among infectious <i>Hp</i> strains. Focusing on a high GC risk area of Northern Italy (Cremona, Lombardy) and using archived paraffin-embedded biopsies, we investigated the associations between the <i>Hp vacA</i> and <i>cagA</i> genotype variants and gastric intraepithelial neoplasia (GIN, 33 cases) versus non-neoplastic gastroduodenal lesions (NNGDLs, 37 cases). The <i>glmM</i> gene and the <i>cagA</i> and <i>vacA</i> (s and m) genotypes were determined by polymerase chain reaction (PCR) and sequencing. <i>Hp</i> was confirmed in 37/37 (100%) NNGDLs and detected in 9/33 GINs (27%), consistently with the well-known <i>Hp</i> loss in GC. <i>CagA</i> was detected in 4/9 <i>Hp</i>-positive GINs and in 29/37 NNGDLs. The <i>vacA</i> s1a and m1 subtypes were more common in GINs than in NNGDLs (6/7 vs. 12/34, p=0.014, for s1a; 7/7 vs. 18/34, p=0.020 for m1), with significant <i>vacA</i> s genotype-specific variance. The GIN-associated <i>vacA</i> s1a sequences clustered together, suggesting that aggressive <i>Hp</i> strains from a unique founder contribute to GC in the high-risk area studied. |
| first_indexed | 2024-04-12T05:29:22Z |
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| id | doaj.art-ca79e6af0a504991bdc0b13f10741a47 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-04-12T05:29:22Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-ca79e6af0a504991bdc0b13f10741a472022-12-22T03:46:08ZengMDPI AGMicroorganisms2076-26072020-02-018332410.3390/microorganisms8030324microorganisms8030324Evolutionarily-Related <i>Helicobacter pylori</i> Genotypes and Gastric Intraepithelial Neoplasia in a High-Risk Area of Northern ItalySonia Toracchio0Rosario Alberto Caruso1Silvia Perconti2Luciana Rigoli3Enrico Betri4Matteo Neri5Fabio Verginelli6Renato Mariani-Costantini7Center for Advanced Studies (CAST/CeSI-MeT), “G. d’Annunzio” University of Chieti-Pescara, 66100 Chieti, ItalyDepartment of Human Pathology in Adult and Developmental Age “Gaetano Barresi”, Section of Pathology, University of Messina, 98123 Messina, ItalyCenter for Advanced Studies (CAST/CeSI-MeT), “G. d’Annunzio” University of Chieti-Pescara, 66100 Chieti, ItalyDepartment of Human Pathology in Adult and Developmental Age “Gaetano Barresi”, Section of Pathology, University of Messina, 98123 Messina, ItalyDepartment of Pathology, Istituti Ospitalieri, 26100 Cremona, ItalyCenter for Advanced Studies (CAST/CeSI-MeT), “G. d’Annunzio” University of Chieti-Pescara, 66100 Chieti, ItalyCenter for Advanced Studies (CAST/CeSI-MeT), “G. d’Annunzio” University of Chieti-Pescara, 66100 Chieti, ItalyCenter for Advanced Studies (CAST/CeSI-MeT), “G. d’Annunzio” University of Chieti-Pescara, 66100 Chieti, Italy<i>Helicobacter pylori</i> (<i>Hp</i>) is the major recognized risk factor for non-cardia gastric cancer (GC), but only a fraction of infected subjects develop GC, thus GC risk might reflect other genetic/environmental cofactors and/or differences in virulence among infectious <i>Hp</i> strains. Focusing on a high GC risk area of Northern Italy (Cremona, Lombardy) and using archived paraffin-embedded biopsies, we investigated the associations between the <i>Hp vacA</i> and <i>cagA</i> genotype variants and gastric intraepithelial neoplasia (GIN, 33 cases) versus non-neoplastic gastroduodenal lesions (NNGDLs, 37 cases). The <i>glmM</i> gene and the <i>cagA</i> and <i>vacA</i> (s and m) genotypes were determined by polymerase chain reaction (PCR) and sequencing. <i>Hp</i> was confirmed in 37/37 (100%) NNGDLs and detected in 9/33 GINs (27%), consistently with the well-known <i>Hp</i> loss in GC. <i>CagA</i> was detected in 4/9 <i>Hp</i>-positive GINs and in 29/37 NNGDLs. The <i>vacA</i> s1a and m1 subtypes were more common in GINs than in NNGDLs (6/7 vs. 12/34, p=0.014, for s1a; 7/7 vs. 18/34, p=0.020 for m1), with significant <i>vacA</i> s genotype-specific variance. The GIN-associated <i>vacA</i> s1a sequences clustered together, suggesting that aggressive <i>Hp</i> strains from a unique founder contribute to GC in the high-risk area studied.https://www.mdpi.com/2076-2607/8/3/324<i>helicobacter pylori</i>virulence factorspathogenicityphylogenetic diversitygastric intraepithelial neoplasiaitaly |
| spellingShingle | Sonia Toracchio Rosario Alberto Caruso Silvia Perconti Luciana Rigoli Enrico Betri Matteo Neri Fabio Verginelli Renato Mariani-Costantini Evolutionarily-Related <i>Helicobacter pylori</i> Genotypes and Gastric Intraepithelial Neoplasia in a High-Risk Area of Northern Italy Microorganisms <i>helicobacter pylori</i> virulence factors pathogenicity phylogenetic diversity gastric intraepithelial neoplasia italy |
| title | Evolutionarily-Related <i>Helicobacter pylori</i> Genotypes and Gastric Intraepithelial Neoplasia in a High-Risk Area of Northern Italy |
| title_full | Evolutionarily-Related <i>Helicobacter pylori</i> Genotypes and Gastric Intraepithelial Neoplasia in a High-Risk Area of Northern Italy |
| title_fullStr | Evolutionarily-Related <i>Helicobacter pylori</i> Genotypes and Gastric Intraepithelial Neoplasia in a High-Risk Area of Northern Italy |
| title_full_unstemmed | Evolutionarily-Related <i>Helicobacter pylori</i> Genotypes and Gastric Intraepithelial Neoplasia in a High-Risk Area of Northern Italy |
| title_short | Evolutionarily-Related <i>Helicobacter pylori</i> Genotypes and Gastric Intraepithelial Neoplasia in a High-Risk Area of Northern Italy |
| title_sort | evolutionarily related i helicobacter pylori i genotypes and gastric intraepithelial neoplasia in a high risk area of northern italy |
| topic | <i>helicobacter pylori</i> virulence factors pathogenicity phylogenetic diversity gastric intraepithelial neoplasia italy |
| url | https://www.mdpi.com/2076-2607/8/3/324 |
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