Optogenetic Activation of A11 Region Increases Motor Activity
Limbic brain regions drive goal-directed behaviors. These behaviors often require dynamic motor responses, but the functional connectome of limbic structures in the diencephalon that control locomotion is not well known. The A11 region, within the posterior diencephalon has been postulated to contri...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-10-01
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| Series: | Frontiers in Neural Circuits |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fncir.2018.00086/full |
| _version_ | 1817996297138864128 |
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| author | Kathrin Koblinger Kathrin Koblinger Céline Jean-Xavier Céline Jean-Xavier Sandeep Sharma Sandeep Sharma Tamás Füzesi Tamás Füzesi Leanne Young Leanne Young Shane E. A. Eaton Shane E. A. Eaton Charlie Hong Ting Kwok Charlie Hong Ting Kwok Jaideep Singh Bains Jaideep Singh Bains Patrick J. Whelan Patrick J. Whelan Patrick J. Whelan |
| author_facet | Kathrin Koblinger Kathrin Koblinger Céline Jean-Xavier Céline Jean-Xavier Sandeep Sharma Sandeep Sharma Tamás Füzesi Tamás Füzesi Leanne Young Leanne Young Shane E. A. Eaton Shane E. A. Eaton Charlie Hong Ting Kwok Charlie Hong Ting Kwok Jaideep Singh Bains Jaideep Singh Bains Patrick J. Whelan Patrick J. Whelan Patrick J. Whelan |
| author_sort | Kathrin Koblinger |
| collection | DOAJ |
| description | Limbic brain regions drive goal-directed behaviors. These behaviors often require dynamic motor responses, but the functional connectome of limbic structures in the diencephalon that control locomotion is not well known. The A11 region, within the posterior diencephalon has been postulated to contribute to motor function and control of pain. Here we show that the A11 region initiates movement. Photostimulation of channelrhodopsin 2 (ChR2) transfected neurons in A11 slice preparations showed that neurons could follow stimulation at frequencies of 20 Hz. Our data show that photostimulation of ChR2 transfected neurons in the A11 region enhances motor activity often leading to locomotion. Using vGluT2-reporter and vGAT-reporter mice we show that the A11 tyrosine hydroxylase positive (TH) dopaminergic neurons are vGluT2 and vGAT negative. We find that in addition to dopaminergic neurons within the A11 region, there is another neuronal subtype which expresses the monoenzymatic aromatic L-amino acid decarboxylase (AADC), but not TH, a key enzyme involved in the synthesis of catecholamines including dopamine. This monoaminergic-based motor circuit may be involved in the control of motor behavior as part of a broader diencephalic motor region. |
| first_indexed | 2024-04-14T02:20:15Z |
| format | Article |
| id | doaj.art-ca7b1618b8144fb788a973f0ba1be452 |
| institution | Directory Open Access Journal |
| issn | 1662-5110 |
| language | English |
| last_indexed | 2024-04-14T02:20:15Z |
| publishDate | 2018-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neural Circuits |
| spelling | doaj.art-ca7b1618b8144fb788a973f0ba1be4522022-12-22T02:18:03ZengFrontiers Media S.A.Frontiers in Neural Circuits1662-51102018-10-011210.3389/fncir.2018.00086370754Optogenetic Activation of A11 Region Increases Motor ActivityKathrin Koblinger0Kathrin Koblinger1Céline Jean-Xavier2Céline Jean-Xavier3Sandeep Sharma4Sandeep Sharma5Tamás Füzesi6Tamás Füzesi7Leanne Young8Leanne Young9Shane E. A. Eaton10Shane E. A. Eaton11Charlie Hong Ting Kwok12Charlie Hong Ting Kwok13Jaideep Singh Bains14Jaideep Singh Bains15Patrick J. Whelan16Patrick J. Whelan17Patrick J. Whelan18Hotchkiss Brain Institute, University of Calgary, Calgary, AB, CanadaDepartment of Comparative Biology and Experimental Medicine, University of Calgary, Calgary, AB, CanadaHotchkiss Brain Institute, University of Calgary, Calgary, AB, CanadaDepartment of Comparative Biology and Experimental Medicine, University of Calgary, Calgary, AB, CanadaHotchkiss Brain Institute, University of Calgary, Calgary, AB, CanadaDepartment of Comparative Biology and Experimental Medicine, University of Calgary, Calgary, AB, CanadaHotchkiss Brain Institute, University of Calgary, Calgary, AB, CanadaDepartment of Physiology and Pharmacology, University of Calgary, Calgary, AB, CanadaHotchkiss Brain Institute, University of Calgary, Calgary, AB, CanadaDepartment of Comparative Biology and Experimental Medicine, University of Calgary, Calgary, AB, CanadaHotchkiss Brain Institute, University of Calgary, Calgary, AB, CanadaDepartment of Comparative Biology and Experimental Medicine, University of Calgary, Calgary, AB, CanadaHotchkiss Brain Institute, University of Calgary, Calgary, AB, CanadaDepartment of Comparative Biology and Experimental Medicine, University of Calgary, Calgary, AB, CanadaHotchkiss Brain Institute, University of Calgary, Calgary, AB, CanadaDepartment of Physiology and Pharmacology, University of Calgary, Calgary, AB, CanadaHotchkiss Brain Institute, University of Calgary, Calgary, AB, CanadaDepartment of Comparative Biology and Experimental Medicine, University of Calgary, Calgary, AB, CanadaDepartment of Physiology and Pharmacology, University of Calgary, Calgary, AB, CanadaLimbic brain regions drive goal-directed behaviors. These behaviors often require dynamic motor responses, but the functional connectome of limbic structures in the diencephalon that control locomotion is not well known. The A11 region, within the posterior diencephalon has been postulated to contribute to motor function and control of pain. Here we show that the A11 region initiates movement. Photostimulation of channelrhodopsin 2 (ChR2) transfected neurons in A11 slice preparations showed that neurons could follow stimulation at frequencies of 20 Hz. Our data show that photostimulation of ChR2 transfected neurons in the A11 region enhances motor activity often leading to locomotion. Using vGluT2-reporter and vGAT-reporter mice we show that the A11 tyrosine hydroxylase positive (TH) dopaminergic neurons are vGluT2 and vGAT negative. We find that in addition to dopaminergic neurons within the A11 region, there is another neuronal subtype which expresses the monoenzymatic aromatic L-amino acid decarboxylase (AADC), but not TH, a key enzyme involved in the synthesis of catecholamines including dopamine. This monoaminergic-based motor circuit may be involved in the control of motor behavior as part of a broader diencephalic motor region.https://www.frontiersin.org/article/10.3389/fncir.2018.00086/fullspinal corddopaminedescendingmotor activitylocomotion control |
| spellingShingle | Kathrin Koblinger Kathrin Koblinger Céline Jean-Xavier Céline Jean-Xavier Sandeep Sharma Sandeep Sharma Tamás Füzesi Tamás Füzesi Leanne Young Leanne Young Shane E. A. Eaton Shane E. A. Eaton Charlie Hong Ting Kwok Charlie Hong Ting Kwok Jaideep Singh Bains Jaideep Singh Bains Patrick J. Whelan Patrick J. Whelan Patrick J. Whelan Optogenetic Activation of A11 Region Increases Motor Activity Frontiers in Neural Circuits spinal cord dopamine descending motor activity locomotion control |
| title | Optogenetic Activation of A11 Region Increases Motor Activity |
| title_full | Optogenetic Activation of A11 Region Increases Motor Activity |
| title_fullStr | Optogenetic Activation of A11 Region Increases Motor Activity |
| title_full_unstemmed | Optogenetic Activation of A11 Region Increases Motor Activity |
| title_short | Optogenetic Activation of A11 Region Increases Motor Activity |
| title_sort | optogenetic activation of a11 region increases motor activity |
| topic | spinal cord dopamine descending motor activity locomotion control |
| url | https://www.frontiersin.org/article/10.3389/fncir.2018.00086/full |
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