Effects of Snake Venom Pharmacopuncture on a Mouse model of Cerebral Infarction
Background This study investigated the effects of Vipera lebetina turanica; snake venom (SV) on cerebral infarction induced by middle cerebral artery occlusion in mice. Methods Following cerebral infarction, SV was injected intravenously or added to BV2 cell culture. Tissue injury was detected using...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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MEDrang Inc.
2019-08-01
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| Series: | Journal of Acupuncture Research |
| Subjects: | |
| Online Access: | http://www.e-jar.org/upload/pdf/jar-2019-00073.pdf |
| _version_ | 1797205317747998720 |
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| author | Chul-Hoon Choi Ho-Sueb Song |
| author_facet | Chul-Hoon Choi Ho-Sueb Song |
| author_sort | Chul-Hoon Choi |
| collection | DOAJ |
| description | Background This study investigated the effects of Vipera lebetina turanica; snake venom (SV) on cerebral infarction induced by middle cerebral artery occlusion in mice. Methods Following cerebral infarction, SV was injected intravenously or added to BV2 cell culture. Tissue injury was detected using triphenyltetrazolium chloride (TTC) staining, neurological deficit score, NO, ROS, and GSH/GSSG assays, qPCR, Western blot, and cell viability. Results Cerebral infarction caused by middle cerebral artery occlusion as observed by TTC staining, showed SV inhibited cell death, reducing the number of brain cells injured due to infarction. SV treatment for cerebral infarction showed a significant decrease in abnormal behavior, as determined by the neurological deficit score. The oxidation and inflammation of the cells that had cerebral infarction caused by middle cerebral artery occlusion (NO assay, ROS, GSH/GSSG assay, and qPCR), showed significant protection by SV. Western blot of brain infarction cells showed the expression of iNOS, COX-2, p-IkB-α, P38, p-JNK, p-ERK to be lower in the SV group. In addition, the expression of IkB increased. BV2 cells were viable when treated with SV at 20 μg/mL or less. Western blot of BV2 cells, treated with 0.625, 1.5, 2.5 μg/mL of SV, showed a significant decrease in the expression of p-IkB-α, p-JNK, iNOS, and COX-2 on BV2 cells induced by LPS. Conclusions SV showed anti-inflammatory and anti-oxidant effects against cerebral infarction and inflammation. |
| first_indexed | 2024-04-24T08:49:12Z |
| format | Article |
| id | doaj.art-ca7f1dd077c94f2fbfbb20ba19051da2 |
| institution | Directory Open Access Journal |
| issn | 2586-288X 2586-2898 |
| language | English |
| last_indexed | 2024-04-24T08:49:12Z |
| publishDate | 2019-08-01 |
| publisher | MEDrang Inc. |
| record_format | Article |
| series | Journal of Acupuncture Research |
| spelling | doaj.art-ca7f1dd077c94f2fbfbb20ba19051da22024-04-16T12:47:19ZengMEDrang Inc.Journal of Acupuncture Research2586-288X2586-28982019-08-0136314014610.13045/jar.2019.000732451Effects of Snake Venom Pharmacopuncture on a Mouse model of Cerebral InfarctionChul-Hoon Choi0Ho-Sueb Song1 Department of Acupuncture & Moxibustion Medicine, College of Korean Medicine, Gachon University, Seongnam, Korea Department of Acupuncture & Moxibustion Medicine, College of Korean Medicine, Gachon University, Seongnam, KoreaBackground This study investigated the effects of Vipera lebetina turanica; snake venom (SV) on cerebral infarction induced by middle cerebral artery occlusion in mice. Methods Following cerebral infarction, SV was injected intravenously or added to BV2 cell culture. Tissue injury was detected using triphenyltetrazolium chloride (TTC) staining, neurological deficit score, NO, ROS, and GSH/GSSG assays, qPCR, Western blot, and cell viability. Results Cerebral infarction caused by middle cerebral artery occlusion as observed by TTC staining, showed SV inhibited cell death, reducing the number of brain cells injured due to infarction. SV treatment for cerebral infarction showed a significant decrease in abnormal behavior, as determined by the neurological deficit score. The oxidation and inflammation of the cells that had cerebral infarction caused by middle cerebral artery occlusion (NO assay, ROS, GSH/GSSG assay, and qPCR), showed significant protection by SV. Western blot of brain infarction cells showed the expression of iNOS, COX-2, p-IkB-α, P38, p-JNK, p-ERK to be lower in the SV group. In addition, the expression of IkB increased. BV2 cells were viable when treated with SV at 20 μg/mL or less. Western blot of BV2 cells, treated with 0.625, 1.5, 2.5 μg/mL of SV, showed a significant decrease in the expression of p-IkB-α, p-JNK, iNOS, and COX-2 on BV2 cells induced by LPS. Conclusions SV showed anti-inflammatory and anti-oxidant effects against cerebral infarction and inflammation.http://www.e-jar.org/upload/pdf/jar-2019-00073.pdfcerebral infarctioninflammationmiddle cerebral artery occlusionpharmacopuncturesnake venom |
| spellingShingle | Chul-Hoon Choi Ho-Sueb Song Effects of Snake Venom Pharmacopuncture on a Mouse model of Cerebral Infarction Journal of Acupuncture Research cerebral infarction inflammation middle cerebral artery occlusion pharmacopuncture snake venom |
| title | Effects of Snake Venom Pharmacopuncture on a Mouse model of Cerebral Infarction |
| title_full | Effects of Snake Venom Pharmacopuncture on a Mouse model of Cerebral Infarction |
| title_fullStr | Effects of Snake Venom Pharmacopuncture on a Mouse model of Cerebral Infarction |
| title_full_unstemmed | Effects of Snake Venom Pharmacopuncture on a Mouse model of Cerebral Infarction |
| title_short | Effects of Snake Venom Pharmacopuncture on a Mouse model of Cerebral Infarction |
| title_sort | effects of snake venom pharmacopuncture on a mouse model of cerebral infarction |
| topic | cerebral infarction inflammation middle cerebral artery occlusion pharmacopuncture snake venom |
| url | http://www.e-jar.org/upload/pdf/jar-2019-00073.pdf |
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