Influences of a fermented milk with Lactobacillus bulgaricus and Streptococcus thermophiles on gut associated lymphoid tissue, mucosal IgA, and gut flora in mice
Summary: Background: Various effects of fermented milks on the immune system have been reported. However, their effects on gut associated lymphoid tissue (GALT) remain controversial. We examined influences of a fermented milk with Lactobacillus bulgaricus and Streptococcus thermophilus on intestina...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-04-01
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| Series: | Clinical Nutrition Open Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667268522000079 |
| _version_ | 1811302900618493952 |
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| author | Haruka Takayama Kazuhiko Fukatsu Kazuya Takahashi Midori Noguchi Ayako Watkins Nana Matsumoto Satoshi Murakoshi |
| author_facet | Haruka Takayama Kazuhiko Fukatsu Kazuya Takahashi Midori Noguchi Ayako Watkins Nana Matsumoto Satoshi Murakoshi |
| author_sort | Haruka Takayama |
| collection | DOAJ |
| description | Summary: Background: Various effects of fermented milks on the immune system have been reported. However, their effects on gut associated lymphoid tissue (GALT) remain controversial. We examined influences of a fermented milk with Lactobacillus bulgaricus and Streptococcus thermophilus on intestinal flora, GALT mass and mucosal IgA levels using uninjured and injured murine models. Methods: Experiment 1: Mice were fed a fermented milk diet containing 20% fermented milk with Lactobacillus bulgaricus and Streptococcus thermophilus (FM group) or an unfermented milk diet (UFM group) for 7 days (Days 0–6) ad libitum. The mice were killed on Day 7, and lymphocyte numbers in Peyer patches (PP), intraepithelial spaces (IE), and the lamina propria (LP) were determined, as well as the phenotypes of these lymphocytes (B cells, CD4+ T cells, CD8+ T cells, αβTCR+ T cells, γδTCR+ T cells), and the mucosal IgA levels in nasal, bronchoalveolar, and intestinal washings. We also measured bacterial flora in the cecum. Experiment 2: Mice were fed the FM or the UFM diet for 7 days ad libitum. On Day 7, the mice underwent gut ischemia reperfusion (IR). On Day 8, the mice were killed, and the same parameters as in experiment 1 were measured. Results: In experiment 1, the percentage of CD4+ T cells in the LP was significantly higher in the FM than in the UFM group. The percentage of bacteria of the order Lactobacillales in cecal contents was higher in the FM than in the UFM group, while the Bacteroides percentage of cecal contents was lower. In experiment 2, the phenotypic expressions of γδ+TCR cells in PP and IE lymphocytes were significantly lower in the FM-IR than in the UFM-IR group. Conclusion: The fermented milk with Lactobacillus bulgaricus and Streptococcus thermophilus examined herein does not clearly exert beneficial effects on GALT lymphocyte cell numbers and mucosal IgA levels in mice regardless of gut flora modulation. |
| first_indexed | 2024-04-13T07:37:23Z |
| format | Article |
| id | doaj.art-ca7fe7f95edb40bfabf6021ce8e2a777 |
| institution | Directory Open Access Journal |
| issn | 2667-2685 |
| language | English |
| last_indexed | 2024-04-13T07:37:23Z |
| publishDate | 2022-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Clinical Nutrition Open Science |
| spelling | doaj.art-ca7fe7f95edb40bfabf6021ce8e2a7772022-12-22T02:56:04ZengElsevierClinical Nutrition Open Science2667-26852022-04-01423648Influences of a fermented milk with Lactobacillus bulgaricus and Streptococcus thermophiles on gut associated lymphoid tissue, mucosal IgA, and gut flora in miceHaruka Takayama0Kazuhiko Fukatsu1Kazuya Takahashi2Midori Noguchi3Ayako Watkins4Nana Matsumoto5Satoshi Murakoshi6Operating Room Management and Surgical Metabolism, Graduate School of Medicine, The University of Tokyo, Tokyo, JapanOperating Room Management and Surgical Metabolism, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan; Surgical Center, The University of Tokyo Hospital, Tokyo, Japan; Corresponding author. Operating Room Management and Surgical Metabolism, Graduate School of Medicine, The University of Tokyo. 7-3-1, Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan.Gastrointestinal Surgery, Graduate School of Medicine, The University of Tokyo, Tokyo, JapanSurgical Center, The University of Tokyo Hospital, Tokyo, JapanOperating Room Management and Surgical Metabolism, Graduate School of Medicine, The University of Tokyo, Tokyo, JapanOperating Room Management and Surgical Metabolism, Graduate School of Medicine, The University of Tokyo, Tokyo, JapanOperating Room Management and Surgical Metabolism, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan; Surgical Center, The University of Tokyo Hospital, Tokyo, JapanSummary: Background: Various effects of fermented milks on the immune system have been reported. However, their effects on gut associated lymphoid tissue (GALT) remain controversial. We examined influences of a fermented milk with Lactobacillus bulgaricus and Streptococcus thermophilus on intestinal flora, GALT mass and mucosal IgA levels using uninjured and injured murine models. Methods: Experiment 1: Mice were fed a fermented milk diet containing 20% fermented milk with Lactobacillus bulgaricus and Streptococcus thermophilus (FM group) or an unfermented milk diet (UFM group) for 7 days (Days 0–6) ad libitum. The mice were killed on Day 7, and lymphocyte numbers in Peyer patches (PP), intraepithelial spaces (IE), and the lamina propria (LP) were determined, as well as the phenotypes of these lymphocytes (B cells, CD4+ T cells, CD8+ T cells, αβTCR+ T cells, γδTCR+ T cells), and the mucosal IgA levels in nasal, bronchoalveolar, and intestinal washings. We also measured bacterial flora in the cecum. Experiment 2: Mice were fed the FM or the UFM diet for 7 days ad libitum. On Day 7, the mice underwent gut ischemia reperfusion (IR). On Day 8, the mice were killed, and the same parameters as in experiment 1 were measured. Results: In experiment 1, the percentage of CD4+ T cells in the LP was significantly higher in the FM than in the UFM group. The percentage of bacteria of the order Lactobacillales in cecal contents was higher in the FM than in the UFM group, while the Bacteroides percentage of cecal contents was lower. In experiment 2, the phenotypic expressions of γδ+TCR cells in PP and IE lymphocytes were significantly lower in the FM-IR than in the UFM-IR group. Conclusion: The fermented milk with Lactobacillus bulgaricus and Streptococcus thermophilus examined herein does not clearly exert beneficial effects on GALT lymphocyte cell numbers and mucosal IgA levels in mice regardless of gut flora modulation.http://www.sciencedirect.com/science/article/pii/S2667268522000079Fermented milkGut associated lymphoid tissueGut floraGut ischemia reperfusionMice |
| spellingShingle | Haruka Takayama Kazuhiko Fukatsu Kazuya Takahashi Midori Noguchi Ayako Watkins Nana Matsumoto Satoshi Murakoshi Influences of a fermented milk with Lactobacillus bulgaricus and Streptococcus thermophiles on gut associated lymphoid tissue, mucosal IgA, and gut flora in mice Clinical Nutrition Open Science Fermented milk Gut associated lymphoid tissue Gut flora Gut ischemia reperfusion Mice |
| title | Influences of a fermented milk with Lactobacillus bulgaricus and Streptococcus thermophiles on gut associated lymphoid tissue, mucosal IgA, and gut flora in mice |
| title_full | Influences of a fermented milk with Lactobacillus bulgaricus and Streptococcus thermophiles on gut associated lymphoid tissue, mucosal IgA, and gut flora in mice |
| title_fullStr | Influences of a fermented milk with Lactobacillus bulgaricus and Streptococcus thermophiles on gut associated lymphoid tissue, mucosal IgA, and gut flora in mice |
| title_full_unstemmed | Influences of a fermented milk with Lactobacillus bulgaricus and Streptococcus thermophiles on gut associated lymphoid tissue, mucosal IgA, and gut flora in mice |
| title_short | Influences of a fermented milk with Lactobacillus bulgaricus and Streptococcus thermophiles on gut associated lymphoid tissue, mucosal IgA, and gut flora in mice |
| title_sort | influences of a fermented milk with lactobacillus bulgaricus and streptococcus thermophiles on gut associated lymphoid tissue mucosal iga and gut flora in mice |
| topic | Fermented milk Gut associated lymphoid tissue Gut flora Gut ischemia reperfusion Mice |
| url | http://www.sciencedirect.com/science/article/pii/S2667268522000079 |
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