Epidemiology of norovirus gastroenteritis in hospitalized children under five years old in western China, 2015–2019
Objectives: Norovirus is associated with one-fifth of all gastroenteritis cases, but basic epidemiological data is lacking, especially in developing countries. As long-term surveillance on norovirus gastroenteritis is scarce in western China, this study aims to update the epidemiological knowledge o...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-10-01
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| Series: | Journal of Microbiology, Immunology and Infection |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1684118221000153 |
| _version_ | 1818890729839132672 |
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| author | Ran-Ran Cao Xiao-Zhen Ma Wan-Yi Li Bao-Ning Wang Yuan Yang Hong-Ren Wang Yu Kuang Jiang-Zhou You Zhong-Yi Zhao Min Ren Lin-Lin Zhou Ming-Yuan Li |
| author_facet | Ran-Ran Cao Xiao-Zhen Ma Wan-Yi Li Bao-Ning Wang Yuan Yang Hong-Ren Wang Yu Kuang Jiang-Zhou You Zhong-Yi Zhao Min Ren Lin-Lin Zhou Ming-Yuan Li |
| author_sort | Ran-Ran Cao |
| collection | DOAJ |
| description | Objectives: Norovirus is associated with one-fifth of all gastroenteritis cases, but basic epidemiological data is lacking, especially in developing countries. As long-term surveillance on norovirus gastroenteritis is scarce in western China, this study aims to update the epidemiological knowledge of norovirus gastroenteritis and to characterize the genotypes of norovirus strains. Methods: Stool samples were collected from hospitalized children under 5 years old with gastroenteritis in Chengdu, China. All samples were tested for norovirus as well as rotavirus, sapovirus, enteric adenovirus, and astrovirus by real-time RT-PCR. RdRp and VP1 genes were sequenced in norovirus-positive samples to investigate viral phylogenies. Results: Of the 1181 samples collected from 2015 to 2019, 242 (20.5%) were positive for norovirus.Among norovirus-positive cases, 65 cases had co-infection with another virus; norovirus/enteric adenovirus was most frequently detected (50.8%, 33/65). The highest positive rate was observed in children aged 13–18 months (23.7%, 68/287). Norovirus infection peaked in autumn (36.6%, 91/249), followed by summer (20.3%, 70/345). Pearson correlation analysis showed significant correlation between the norovirus-positive rate and humidity (r = 0.773, P < 0.05). GII.4 Sydney 2012 [P31] (48.5%, 79/163) and GII.3 [P12] (35.6%, 58/163) were the dominant norovirus strains. Conclusions: Norovirus has become one of the most common causes of viral gastroenteritis in children under 5 years old in western China. Continuous monitoring is imperative for predicting the emergence of new epidemic strains and for current vaccine development. |
| first_indexed | 2024-12-19T17:29:33Z |
| format | Article |
| id | doaj.art-ca83610081bc483c8e1d5c79496ca8fb |
| institution | Directory Open Access Journal |
| issn | 1684-1182 |
| language | English |
| last_indexed | 2024-12-19T17:29:33Z |
| publishDate | 2021-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Microbiology, Immunology and Infection |
| spelling | doaj.art-ca83610081bc483c8e1d5c79496ca8fb2022-12-21T20:12:29ZengElsevierJournal of Microbiology, Immunology and Infection1684-11822021-10-01545918925Epidemiology of norovirus gastroenteritis in hospitalized children under five years old in western China, 2015–2019Ran-Ran Cao0Xiao-Zhen Ma1Wan-Yi Li2Bao-Ning Wang3Yuan Yang4Hong-Ren Wang5Yu Kuang6Jiang-Zhou You7Zhong-Yi Zhao8Min Ren9Lin-Lin Zhou10Ming-Yuan Li11West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, People's Republic of China; Sichuan Center for Disease Control and Prevention, Chengdu 610041, People's Republic of ChinaSichuan Center for Disease Control and Prevention, Chengdu 610041, People's Republic of ChinaWest China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, People's Republic of ChinaWest China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, People's Republic of ChinaWest China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, People's Republic of ChinaWest China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, People's Republic of ChinaWest China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, People's Republic of ChinaWest China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, People's Republic of ChinaWest China Hospital, Sichuan University, Chengdu 610041, People's Republic of ChinaSichuan Center for Disease Control and Prevention, Chengdu 610041, People's Republic of ChinaWest China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, People's Republic of China; Corresponding author. West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, No.17 People's South Road, Chengdu 610041, People's Republic of China.West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, Chengdu 610041, People's Republic of China; Corresponding author. West China School of Basic Medical Sciences & Forensic Medicine, Sichuan University, No.17 People's South Road, Chengdu 610041, People's Republic of China.Objectives: Norovirus is associated with one-fifth of all gastroenteritis cases, but basic epidemiological data is lacking, especially in developing countries. As long-term surveillance on norovirus gastroenteritis is scarce in western China, this study aims to update the epidemiological knowledge of norovirus gastroenteritis and to characterize the genotypes of norovirus strains. Methods: Stool samples were collected from hospitalized children under 5 years old with gastroenteritis in Chengdu, China. All samples were tested for norovirus as well as rotavirus, sapovirus, enteric adenovirus, and astrovirus by real-time RT-PCR. RdRp and VP1 genes were sequenced in norovirus-positive samples to investigate viral phylogenies. Results: Of the 1181 samples collected from 2015 to 2019, 242 (20.5%) were positive for norovirus.Among norovirus-positive cases, 65 cases had co-infection with another virus; norovirus/enteric adenovirus was most frequently detected (50.8%, 33/65). The highest positive rate was observed in children aged 13–18 months (23.7%, 68/287). Norovirus infection peaked in autumn (36.6%, 91/249), followed by summer (20.3%, 70/345). Pearson correlation analysis showed significant correlation between the norovirus-positive rate and humidity (r = 0.773, P < 0.05). GII.4 Sydney 2012 [P31] (48.5%, 79/163) and GII.3 [P12] (35.6%, 58/163) were the dominant norovirus strains. Conclusions: Norovirus has become one of the most common causes of viral gastroenteritis in children under 5 years old in western China. Continuous monitoring is imperative for predicting the emergence of new epidemic strains and for current vaccine development.http://www.sciencedirect.com/science/article/pii/S1684118221000153NorovirusGastroenteritisEpidemiologyGenotypesChildrenWest China |
| spellingShingle | Ran-Ran Cao Xiao-Zhen Ma Wan-Yi Li Bao-Ning Wang Yuan Yang Hong-Ren Wang Yu Kuang Jiang-Zhou You Zhong-Yi Zhao Min Ren Lin-Lin Zhou Ming-Yuan Li Epidemiology of norovirus gastroenteritis in hospitalized children under five years old in western China, 2015–2019 Journal of Microbiology, Immunology and Infection Norovirus Gastroenteritis Epidemiology Genotypes Children West China |
| title | Epidemiology of norovirus gastroenteritis in hospitalized children under five years old in western China, 2015–2019 |
| title_full | Epidemiology of norovirus gastroenteritis in hospitalized children under five years old in western China, 2015–2019 |
| title_fullStr | Epidemiology of norovirus gastroenteritis in hospitalized children under five years old in western China, 2015–2019 |
| title_full_unstemmed | Epidemiology of norovirus gastroenteritis in hospitalized children under five years old in western China, 2015–2019 |
| title_short | Epidemiology of norovirus gastroenteritis in hospitalized children under five years old in western China, 2015–2019 |
| title_sort | epidemiology of norovirus gastroenteritis in hospitalized children under five years old in western china 2015 2019 |
| topic | Norovirus Gastroenteritis Epidemiology Genotypes Children West China |
| url | http://www.sciencedirect.com/science/article/pii/S1684118221000153 |
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