Baicalin suppress the development of polycystic ovary syndrome via regulating the miR-874-3p/FOXO3 and miR-144/FOXO1 axis
AbstractContext Polycystic ovary syndrome (PCOS) is a common and complex disease caused by endocrine and metabolic dysfunction in women of reproductive age. Baicalin is reported to ameliorate PCOS.Objective This study determines whether baicalin could affect the progression of PCOS.Materials and met...
| Հիմնական հեղինակներ: | , , , |
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| Ձևաչափ: | Հոդված |
| Լեզու: | English |
| Հրապարակվել է: |
Taylor & Francis Group
2023-12-01
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| Շարք: | Pharmaceutical Biology |
| Խորագրեր: | |
| Առցանց հասանելիություն: | https://www.tandfonline.com/doi/10.1080/13880209.2023.2208636 |
| _version_ | 1827302107869347840 |
|---|---|
| author | Xiaoyuan Xu Xiaohua Xu Xiaoshuang Wang Ling Shen |
| author_facet | Xiaoyuan Xu Xiaohua Xu Xiaoshuang Wang Ling Shen |
| author_sort | Xiaoyuan Xu |
| collection | DOAJ |
| description | AbstractContext Polycystic ovary syndrome (PCOS) is a common and complex disease caused by endocrine and metabolic dysfunction in women of reproductive age. Baicalin is reported to ameliorate PCOS.Objective This study determines whether baicalin could affect the progression of PCOS.Materials and methods To establish an animal model of PCOS, female Sprague-Dawley (SD) rats were subcutaneously injected with dehydroepiandrosterone (DHEA, 60 mg/kg) for 20 days. Next, normal and PCOS mice were divided into 3 groups: control, PCOS, PCOS + Baicalin (20 mg/kg) groups. In addition, the levels of microRNA-874-3p (miR-874-3p) and microRNA-144 (miR-144) in ovarian tissues were assessed by reverse transcription-quantitative polymerase chain reaction (RT-qPCR).Results Compared to the PCOS group, baicalin treatment significantly declined free testosterone (33.71 pg/mL vs. 56.05 pg/mL) and luteinizing hormone (LH; 3971.73 pg/mL vs. 5201.50 pg/mL) levels in rats with PCOS. Additionally, compared to the control group, 100 μM baicalin lessened miR-874-3p and miR-144 levels in human ovarian granulosa cells (KGN cells) by 36.87% and 32.57%, respectively. Furthermore, forkhead box O (FOXO) proteins FOXO1 and FOXO3 are the direct targets of miR-144 and miR-874-3p, respectively. Meanwhile, baicalin induced G0-G1 phase arrest (69.56 ± 3.7% at baicalin with 100 μM vs. 51.24 ± 3.2%, control) in KGN cells correlating with decreased p27 Kip1 (FOXO proteins downstream effector gene) expression by 55.5%; however, miR-874-3p or miR-144 overexpression could abolish this effect.Conclusions Baicalin could alleviate the symptoms of PCOS via regulating miR-874-3p/FOXO3 and miR-144/FOXO1 axis, demonstrating its potential utility in PCOS treatment. |
| first_indexed | 2024-03-09T00:09:36Z |
| format | Article |
| id | doaj.art-ca8581294703406a9d35f2d0174cae1f |
| institution | Directory Open Access Journal |
| issn | 1388-0209 1744-5116 |
| language | English |
| last_indexed | 2024-04-24T16:38:49Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Pharmaceutical Biology |
| spelling | doaj.art-ca8581294703406a9d35f2d0174cae1f2024-03-29T11:10:26ZengTaylor & Francis GroupPharmaceutical Biology1388-02091744-51162023-12-0161187888510.1080/13880209.2023.2208636Baicalin suppress the development of polycystic ovary syndrome via regulating the miR-874-3p/FOXO3 and miR-144/FOXO1 axisXiaoyuan Xu0Xiaohua Xu1Xiaoshuang Wang2Ling Shen3Department of Obstetrics and Gynecology, The First Affiliated Hospital of Shantou University Medical College, Shantou, ChinaDepartment of Cardiology, the First People’s Hospital of Jingdezhen, Jingdezhen, ChinaDepartment of Obstetrics and Gynecology, The First Affiliated Hospital of Shantou University Medical College, Shantou, ChinaDepartment of Obstetrics and Gynecology, The First Affiliated Hospital of Shantou University Medical College, Shantou, ChinaAbstractContext Polycystic ovary syndrome (PCOS) is a common and complex disease caused by endocrine and metabolic dysfunction in women of reproductive age. Baicalin is reported to ameliorate PCOS.Objective This study determines whether baicalin could affect the progression of PCOS.Materials and methods To establish an animal model of PCOS, female Sprague-Dawley (SD) rats were subcutaneously injected with dehydroepiandrosterone (DHEA, 60 mg/kg) for 20 days. Next, normal and PCOS mice were divided into 3 groups: control, PCOS, PCOS + Baicalin (20 mg/kg) groups. In addition, the levels of microRNA-874-3p (miR-874-3p) and microRNA-144 (miR-144) in ovarian tissues were assessed by reverse transcription-quantitative polymerase chain reaction (RT-qPCR).Results Compared to the PCOS group, baicalin treatment significantly declined free testosterone (33.71 pg/mL vs. 56.05 pg/mL) and luteinizing hormone (LH; 3971.73 pg/mL vs. 5201.50 pg/mL) levels in rats with PCOS. Additionally, compared to the control group, 100 μM baicalin lessened miR-874-3p and miR-144 levels in human ovarian granulosa cells (KGN cells) by 36.87% and 32.57%, respectively. Furthermore, forkhead box O (FOXO) proteins FOXO1 and FOXO3 are the direct targets of miR-144 and miR-874-3p, respectively. Meanwhile, baicalin induced G0-G1 phase arrest (69.56 ± 3.7% at baicalin with 100 μM vs. 51.24 ± 3.2%, control) in KGN cells correlating with decreased p27 Kip1 (FOXO proteins downstream effector gene) expression by 55.5%; however, miR-874-3p or miR-144 overexpression could abolish this effect.Conclusions Baicalin could alleviate the symptoms of PCOS via regulating miR-874-3p/FOXO3 and miR-144/FOXO1 axis, demonstrating its potential utility in PCOS treatment.https://www.tandfonline.com/doi/10.1080/13880209.2023.2208636Endocrine and metabolic dysfunctionmiRNAScutellaria baicalensis |
| spellingShingle | Xiaoyuan Xu Xiaohua Xu Xiaoshuang Wang Ling Shen Baicalin suppress the development of polycystic ovary syndrome via regulating the miR-874-3p/FOXO3 and miR-144/FOXO1 axis Pharmaceutical Biology Endocrine and metabolic dysfunction miRNA Scutellaria baicalensis |
| title | Baicalin suppress the development of polycystic ovary syndrome via regulating the miR-874-3p/FOXO3 and miR-144/FOXO1 axis |
| title_full | Baicalin suppress the development of polycystic ovary syndrome via regulating the miR-874-3p/FOXO3 and miR-144/FOXO1 axis |
| title_fullStr | Baicalin suppress the development of polycystic ovary syndrome via regulating the miR-874-3p/FOXO3 and miR-144/FOXO1 axis |
| title_full_unstemmed | Baicalin suppress the development of polycystic ovary syndrome via regulating the miR-874-3p/FOXO3 and miR-144/FOXO1 axis |
| title_short | Baicalin suppress the development of polycystic ovary syndrome via regulating the miR-874-3p/FOXO3 and miR-144/FOXO1 axis |
| title_sort | baicalin suppress the development of polycystic ovary syndrome via regulating the mir 874 3p foxo3 and mir 144 foxo1 axis |
| topic | Endocrine and metabolic dysfunction miRNA Scutellaria baicalensis |
| url | https://www.tandfonline.com/doi/10.1080/13880209.2023.2208636 |
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