Isolation and characterization of a novel alphanodavirus

<p>Abstract</p> <p>Background</p> <p><it>Nodaviridae </it>is a family of non-enveloped isometric viruses with bipartite positive-sense RNA genomes. The <it>Nodaviridae </it>family consists of two genera: alpha- and beta-nodavirus. Alphanodaviruse...

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Main Authors: Mao Haitao, Li Xiang, Wang Yun, Bai Huimin, Li Yan, Han Shili, Shi Zhengli, Chen Xinwen
Format: Article
Language:English
Published: BMC 2011-06-01
Series:Virology Journal
Subjects:
Online Access:http://www.virologyj.com/content/8/1/311
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author Mao Haitao
Li Xiang
Wang Yun
Bai Huimin
Li Yan
Han Shili
Shi Zhengli
Chen Xinwen
author_facet Mao Haitao
Li Xiang
Wang Yun
Bai Huimin
Li Yan
Han Shili
Shi Zhengli
Chen Xinwen
author_sort Mao Haitao
collection DOAJ
description <p>Abstract</p> <p>Background</p> <p><it>Nodaviridae </it>is a family of non-enveloped isometric viruses with bipartite positive-sense RNA genomes. The <it>Nodaviridae </it>family consists of two genera: alpha- and beta-nodavirus. Alphanodaviruses usually infect insect cells. Some commercially available insect cell lines have been latently infected by Alphanodaviruses.</p> <p>Results</p> <p>A non-enveloped small virus of approximately 30 nm in diameter was discovered co-existing with a recombinant <it>Helicoverpa armigera </it>single nucleopolyhedrovirus (<it>Hear</it>NPV) in Hz-AM1 cells. Genome sequencing and phylogenetic assays indicate that this novel virus belongs to the genus of alphanodavirus in the family <it>Nodaviridae </it>and was designated HzNV. HzNV possesses a RNA genome that contains two segments. RNA1 is 3038 nt long and encodes a 110 kDa viral protein termed protein A. The 1404 nt long RNA2 encodes a 44 kDa protein, which exhibits a high homology with coat protein precursors of other alphanodaviruses. HzNV virions were located in the cytoplasm, in association with cytoplasmic membrane structures. The host susceptibility test demonstrated that HzNV was able to infect various cell lines ranging from insect cells to mammalian cells. However, only Hz-AM1 appeared to be fully permissive for HzNV, as the mature viral coat protein essential for HzNV particle formation was limited to Hz-AM1 cells.</p> <p>Conclusion</p> <p>A novel alphanodavirus, which is 30 nm in diameter and with a limited host range, was discovered in Hz-AM1 cells.</p>
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spelling doaj.art-ca86ee65e7bf4cb1814ce2208047b2702022-12-22T00:27:11ZengBMCVirology Journal1743-422X2011-06-018131110.1186/1743-422X-8-311Isolation and characterization of a novel alphanodavirusMao HaitaoLi XiangWang YunBai HuiminLi YanHan ShiliShi ZhengliChen Xinwen<p>Abstract</p> <p>Background</p> <p><it>Nodaviridae </it>is a family of non-enveloped isometric viruses with bipartite positive-sense RNA genomes. The <it>Nodaviridae </it>family consists of two genera: alpha- and beta-nodavirus. Alphanodaviruses usually infect insect cells. Some commercially available insect cell lines have been latently infected by Alphanodaviruses.</p> <p>Results</p> <p>A non-enveloped small virus of approximately 30 nm in diameter was discovered co-existing with a recombinant <it>Helicoverpa armigera </it>single nucleopolyhedrovirus (<it>Hear</it>NPV) in Hz-AM1 cells. Genome sequencing and phylogenetic assays indicate that this novel virus belongs to the genus of alphanodavirus in the family <it>Nodaviridae </it>and was designated HzNV. HzNV possesses a RNA genome that contains two segments. RNA1 is 3038 nt long and encodes a 110 kDa viral protein termed protein A. The 1404 nt long RNA2 encodes a 44 kDa protein, which exhibits a high homology with coat protein precursors of other alphanodaviruses. HzNV virions were located in the cytoplasm, in association with cytoplasmic membrane structures. The host susceptibility test demonstrated that HzNV was able to infect various cell lines ranging from insect cells to mammalian cells. However, only Hz-AM1 appeared to be fully permissive for HzNV, as the mature viral coat protein essential for HzNV particle formation was limited to Hz-AM1 cells.</p> <p>Conclusion</p> <p>A novel alphanodavirus, which is 30 nm in diameter and with a limited host range, was discovered in Hz-AM1 cells.</p>http://www.virologyj.com/content/8/1/311AlphanodavirusHzNVHz-AM1Host susceptibility
spellingShingle Mao Haitao
Li Xiang
Wang Yun
Bai Huimin
Li Yan
Han Shili
Shi Zhengli
Chen Xinwen
Isolation and characterization of a novel alphanodavirus
Virology Journal
Alphanodavirus
HzNV
Hz-AM1
Host susceptibility
title Isolation and characterization of a novel alphanodavirus
title_full Isolation and characterization of a novel alphanodavirus
title_fullStr Isolation and characterization of a novel alphanodavirus
title_full_unstemmed Isolation and characterization of a novel alphanodavirus
title_short Isolation and characterization of a novel alphanodavirus
title_sort isolation and characterization of a novel alphanodavirus
topic Alphanodavirus
HzNV
Hz-AM1
Host susceptibility
url http://www.virologyj.com/content/8/1/311
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AT lixiang isolationandcharacterizationofanovelalphanodavirus
AT wangyun isolationandcharacterizationofanovelalphanodavirus
AT baihuimin isolationandcharacterizationofanovelalphanodavirus
AT liyan isolationandcharacterizationofanovelalphanodavirus
AT hanshili isolationandcharacterizationofanovelalphanodavirus
AT shizhengli isolationandcharacterizationofanovelalphanodavirus
AT chenxinwen isolationandcharacterizationofanovelalphanodavirus