An interpromoter region enhances transcription of argk gene, encoding the phaseolotoxin-resistant ornithine carbamoyltransferase in Pseudomonas syringae pv. phaseolicola
Pseudomonas syringae pv. phaseolicola, is the causal agent of halo blight on bean, a disease characterized by water soaked lesions surrounded by a chlorotic halo, which is produced by the action of phaseolotoxin, a nonhost-specific toxin optimally produced between 18°C to 20°C. To protect itself aga...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Universidad Nacional Autónoma de México
2018-08-01
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| Series: | TIP Revista Especializada en Ciencias Químico-Biológicas |
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| Online Access: | http://tip.zaragoza.unam.mx/index.php/tip/article/view/148 |
| _version_ | 1818175814484623360 |
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| author | José Luis Hernández-Flores Ariel Álvarez-Morales Susana De la Torre-Zavala Laura Hernández-Soriano Alejandra Chacón-López Efigenia Montalvo-González Selene Aguilera |
| author_facet | José Luis Hernández-Flores Ariel Álvarez-Morales Susana De la Torre-Zavala Laura Hernández-Soriano Alejandra Chacón-López Efigenia Montalvo-González Selene Aguilera |
| author_sort | José Luis Hernández-Flores |
| collection | DOAJ |
| description | Pseudomonas syringae pv. phaseolicola, is the causal agent of halo blight on bean, a disease characterized by water soaked lesions surrounded by a chlorotic halo, which is produced by the action of phaseolotoxin, a nonhost-specific toxin optimally produced between 18°C to 20°C. To protect itself against phaseolotoxin, P. syringae pv. phaseolicola possesses a phaseolotoxin-resistant OCTase (ROCT) encoded by argK, which is expressed under conditions leading to the synthesis of phaseolotoxin. The promoter of argK is divergent to that of phtABC genes and both share an interpromoter region. The objective of the present study was to evaluate the role of the interpromoter region on argK transcription in P. syringae pv. phaseolicola. To this, deletions of the interpromoter region were made and argK expression was evaluated in an heterologous system. Also, the effect of multiple copies of the interpromoter region on phaseolotoxin production was determined. The obtained results showed that this region is necessary to enhance the transcription of argK. Additionally, this region in a multiple copies, interfered with the thermoregulation of phaseolotoxin at 28°C in strain P. syringae pv. phaseolicola NPS3121. |
| first_indexed | 2024-12-11T20:06:16Z |
| format | Article |
| id | doaj.art-ca8c3114a64042e7b8d862c484592a97 |
| institution | Directory Open Access Journal |
| issn | 1405-888X 2395-8723 |
| language | English |
| last_indexed | 2024-12-11T20:06:16Z |
| publishDate | 2018-08-01 |
| publisher | Universidad Nacional Autónoma de México |
| record_format | Article |
| series | TIP Revista Especializada en Ciencias Químico-Biológicas |
| spelling | doaj.art-ca8c3114a64042e7b8d862c484592a972022-12-22T00:52:23ZengUniversidad Nacional Autónoma de MéxicoTIP Revista Especializada en Ciencias Químico-Biológicas1405-888X2395-87232018-08-0121Supl 1384510.22201/fesz.23958723e.2018.0.148An interpromoter region enhances transcription of argk gene, encoding the phaseolotoxin-resistant ornithine carbamoyltransferase in Pseudomonas syringae pv. phaseolicolaJosé Luis Hernández-Flores0Ariel Álvarez-Morales1Susana De la Torre-Zavala2Laura Hernández-Soriano3Alejandra Chacón-López4Efigenia Montalvo-González5Selene Aguilera6Departamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV)Departamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV)Instituto de Biotecnología, Facultad de Ciencias Biológicas, Ciudad Universitaria, Universidad Autónoma de Nuevo LeónLaboratorio Integral de Investigación en Alimentos, Instituto Tecnológico de TepicLaboratorio Integral de Investigación en Alimentos, Instituto Tecnológico de TepicLaboratorio Integral de Investigación en Alimentos, Instituto Tecnológico de TepicLaboratorio Integral de Investigación en Alimentos, CONACYT-Instituto Tecnológico de TepicPseudomonas syringae pv. phaseolicola, is the causal agent of halo blight on bean, a disease characterized by water soaked lesions surrounded by a chlorotic halo, which is produced by the action of phaseolotoxin, a nonhost-specific toxin optimally produced between 18°C to 20°C. To protect itself against phaseolotoxin, P. syringae pv. phaseolicola possesses a phaseolotoxin-resistant OCTase (ROCT) encoded by argK, which is expressed under conditions leading to the synthesis of phaseolotoxin. The promoter of argK is divergent to that of phtABC genes and both share an interpromoter region. The objective of the present study was to evaluate the role of the interpromoter region on argK transcription in P. syringae pv. phaseolicola. To this, deletions of the interpromoter region were made and argK expression was evaluated in an heterologous system. Also, the effect of multiple copies of the interpromoter region on phaseolotoxin production was determined. The obtained results showed that this region is necessary to enhance the transcription of argK. Additionally, this region in a multiple copies, interfered with the thermoregulation of phaseolotoxin at 28°C in strain P. syringae pv. phaseolicola NPS3121.http://tip.zaragoza.unam.mx/index.php/tip/article/view/148phaseolotoxinargk genepromoterthermoregulationinterpromoter region |
| spellingShingle | José Luis Hernández-Flores Ariel Álvarez-Morales Susana De la Torre-Zavala Laura Hernández-Soriano Alejandra Chacón-López Efigenia Montalvo-González Selene Aguilera An interpromoter region enhances transcription of argk gene, encoding the phaseolotoxin-resistant ornithine carbamoyltransferase in Pseudomonas syringae pv. phaseolicola TIP Revista Especializada en Ciencias Químico-Biológicas phaseolotoxin argk gene promoter thermoregulation interpromoter region |
| title | An interpromoter region enhances transcription of argk gene, encoding the phaseolotoxin-resistant ornithine carbamoyltransferase in Pseudomonas syringae pv. phaseolicola |
| title_full | An interpromoter region enhances transcription of argk gene, encoding the phaseolotoxin-resistant ornithine carbamoyltransferase in Pseudomonas syringae pv. phaseolicola |
| title_fullStr | An interpromoter region enhances transcription of argk gene, encoding the phaseolotoxin-resistant ornithine carbamoyltransferase in Pseudomonas syringae pv. phaseolicola |
| title_full_unstemmed | An interpromoter region enhances transcription of argk gene, encoding the phaseolotoxin-resistant ornithine carbamoyltransferase in Pseudomonas syringae pv. phaseolicola |
| title_short | An interpromoter region enhances transcription of argk gene, encoding the phaseolotoxin-resistant ornithine carbamoyltransferase in Pseudomonas syringae pv. phaseolicola |
| title_sort | interpromoter region enhances transcription of argk gene encoding the phaseolotoxin resistant ornithine carbamoyltransferase in pseudomonas syringae pv phaseolicola |
| topic | phaseolotoxin argk gene promoter thermoregulation interpromoter region |
| url | http://tip.zaragoza.unam.mx/index.php/tip/article/view/148 |
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