Risk estimation of HNA-3 incompatibility and alloimmunization in Thai populations.
Severe transfusion-related acute lung injury (TRALI) is often due to antibodies in blood components directed against human neutrophil antigen (HNA)-3a. This study aimed to report the genotype frequencies of the HNA-3 system and to estimate the potential risk of HNA-3 incompatibility and alloimmuniza...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4301801?pdf=render |
| _version_ | 1818018951317159936 |
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| author | Oytip Nathalang Kamphon Intharanut Kanokpol Siriphanthong Siriporn Nathalang Nipapan Leetrakool |
| author_facet | Oytip Nathalang Kamphon Intharanut Kanokpol Siriphanthong Siriporn Nathalang Nipapan Leetrakool |
| author_sort | Oytip Nathalang |
| collection | DOAJ |
| description | Severe transfusion-related acute lung injury (TRALI) is often due to antibodies in blood components directed against human neutrophil antigen (HNA)-3a. This study aimed to report the genotype frequencies of the HNA-3 system and to estimate the potential risk of HNA-3 incompatibility and alloimmunization in two Thai populations. Eight hundred DNA samples obtained from 500 unrelated healthy blood donors at the National Blood Centre, Thai Red Cross Society, Bangkok and 300 samples from the Blood Bank, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand were included. HNA-3 genotyping was performed using an in-house polymerase chain reaction with sequence-specific primer (PCR-SSP) technique. The observed frequencies of the HNA-3a/3a, HNA-3a/3b, and HNA-3b/3b genotypes were 0.528, 0.380, and 0.092 in central Thais and 0.600, 0.350, and 0.050 in northern Thais, respectively. The frequencies were used to estimate HNA-3 incompatibility and risk of HNA-3a alloimmunization. The HNA-3 incompatibility in central Thais (33.28%) was higher than northern Thais (28.75%), corresponding to a significantly higher probability of HNA-3a alloimmunization (P<0.05) similar to Japanese and Chinese populations. This study showed the high risk of HNA-3 incompatibility and alloimmunization, especially in central Thai blood donors. A molecular-based identification of the HNA-3 genotype of female donors is suggested to reduce the risk of TRALI following plasma and whole blood allogeneic transfusion. |
| first_indexed | 2024-04-14T07:46:30Z |
| format | Article |
| id | doaj.art-ca90ca003c684b57ac09179910b0db99 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-14T07:46:30Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ca90ca003c684b57ac09179910b0db992022-12-22T02:05:20ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01101e011690510.1371/journal.pone.0116905Risk estimation of HNA-3 incompatibility and alloimmunization in Thai populations.Oytip NathalangKamphon IntharanutKanokpol SiriphanthongSiriporn NathalangNipapan LeetrakoolSevere transfusion-related acute lung injury (TRALI) is often due to antibodies in blood components directed against human neutrophil antigen (HNA)-3a. This study aimed to report the genotype frequencies of the HNA-3 system and to estimate the potential risk of HNA-3 incompatibility and alloimmunization in two Thai populations. Eight hundred DNA samples obtained from 500 unrelated healthy blood donors at the National Blood Centre, Thai Red Cross Society, Bangkok and 300 samples from the Blood Bank, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand were included. HNA-3 genotyping was performed using an in-house polymerase chain reaction with sequence-specific primer (PCR-SSP) technique. The observed frequencies of the HNA-3a/3a, HNA-3a/3b, and HNA-3b/3b genotypes were 0.528, 0.380, and 0.092 in central Thais and 0.600, 0.350, and 0.050 in northern Thais, respectively. The frequencies were used to estimate HNA-3 incompatibility and risk of HNA-3a alloimmunization. The HNA-3 incompatibility in central Thais (33.28%) was higher than northern Thais (28.75%), corresponding to a significantly higher probability of HNA-3a alloimmunization (P<0.05) similar to Japanese and Chinese populations. This study showed the high risk of HNA-3 incompatibility and alloimmunization, especially in central Thai blood donors. A molecular-based identification of the HNA-3 genotype of female donors is suggested to reduce the risk of TRALI following plasma and whole blood allogeneic transfusion.http://europepmc.org/articles/PMC4301801?pdf=render |
| spellingShingle | Oytip Nathalang Kamphon Intharanut Kanokpol Siriphanthong Siriporn Nathalang Nipapan Leetrakool Risk estimation of HNA-3 incompatibility and alloimmunization in Thai populations. PLoS ONE |
| title | Risk estimation of HNA-3 incompatibility and alloimmunization in Thai populations. |
| title_full | Risk estimation of HNA-3 incompatibility and alloimmunization in Thai populations. |
| title_fullStr | Risk estimation of HNA-3 incompatibility and alloimmunization in Thai populations. |
| title_full_unstemmed | Risk estimation of HNA-3 incompatibility and alloimmunization in Thai populations. |
| title_short | Risk estimation of HNA-3 incompatibility and alloimmunization in Thai populations. |
| title_sort | risk estimation of hna 3 incompatibility and alloimmunization in thai populations |
| url | http://europepmc.org/articles/PMC4301801?pdf=render |
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