Molecular characterization of Turnip yellows virus: A new pathogen of mustards in Serbia
In 2018, a total of 58 mustard samples from two different localities in Serbia (Rimski Šančevi and Senta) were collected and tested for the presence of Turnip yellow virus (TuYV), Cauliflower mosaic virus (CaMV) and Turnip mosaic virus (TuMV) by DAS-ELTSA. TuYV was serologically detected in 42 teste...
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| Format: | Article |
| Language: | English |
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Institute of field and vegetable crops, Novi Sad
2019-01-01
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| Series: | Ratarstvo i Povrtarstvo |
| Subjects: | |
| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/1821-3944/2019/1821-39441903082M.pdf |
| _version_ | 1818613372477767680 |
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| author | Milošević Dragana Ignjatov Maja Marjanović-Jeromela Ana Stanković Ivana Nikolić Zorica Tamindžić Gordana Krstić Branka |
| author_facet | Milošević Dragana Ignjatov Maja Marjanović-Jeromela Ana Stanković Ivana Nikolić Zorica Tamindžić Gordana Krstić Branka |
| author_sort | Milošević Dragana |
| collection | DOAJ |
| description | In 2018, a total of 58 mustard samples from two different localities in Serbia (Rimski Šančevi and Senta) were collected and tested for the presence of Turnip yellow virus (TuYV), Cauliflower mosaic virus (CaMV) and Turnip mosaic virus (TuMV) by DAS-ELTSA. TuYV was serologically detected in 42 tested samples while all collected samples were negative for CaMV and TuMV. By using aphid transmission tests, five test plants were inoculated with two ELTSApositive TuYV samples of naturally infected mustard plants. Virus species identification was performed by amplification of a 780 bp fragment in all tested samples using the specific primers TuYVorf0F/TuYVorf0R. The RTPCR products from two isolates 88Sal (MK144816) and 98Bni (MK144817) were sequenced and compared with the GenBank sequences of TuYV. Serbian isolates showed the highest identity with Poland TuYV isolate (EU022489). Phylogenetic analysis showed that TuYV isolates from Serbia were clustered with other TuYV sequences retrieved from the GenBank. |
| first_indexed | 2024-12-16T16:01:04Z |
| format | Article |
| id | doaj.art-ca91f04c42af49b18302a9cf473d406f |
| institution | Directory Open Access Journal |
| issn | 1821-3944 2217-8392 |
| language | English |
| last_indexed | 2024-12-16T16:01:04Z |
| publishDate | 2019-01-01 |
| publisher | Institute of field and vegetable crops, Novi Sad |
| record_format | Article |
| series | Ratarstvo i Povrtarstvo |
| spelling | doaj.art-ca91f04c42af49b18302a9cf473d406f2022-12-21T22:25:28ZengInstitute of field and vegetable crops, Novi SadRatarstvo i Povrtarstvo1821-39442217-83922019-01-0156382871821-39441903082MMolecular characterization of Turnip yellows virus: A new pathogen of mustards in SerbiaMilošević Dragana0Ignjatov Maja1https://orcid.org/0000-0003-4650-5082Marjanović-Jeromela Ana2https://orcid.org/0000-0002-7663-0696Stanković Ivana3Nikolić Zorica4Tamindžić Gordana5https://orcid.org/0000-0002-6808-0129Krstić Branka6Institute of Field and Vegetable Crops, Novi SadInstitute of Field and Vegetable Crops, Novi SadInstitute of Field and Vegetable Crops, Novi SadUniversity of Belgrade, Faculty of Agriculture, Institute of PhytomedicineInstitute of Field and Vegetable Crops, Novi SadInstitute of Field and Vegetable Crops, Novi SadUniversity of Belgrade, Faculty of Agriculture, Institute of PhytomedicineIn 2018, a total of 58 mustard samples from two different localities in Serbia (Rimski Šančevi and Senta) were collected and tested for the presence of Turnip yellow virus (TuYV), Cauliflower mosaic virus (CaMV) and Turnip mosaic virus (TuMV) by DAS-ELTSA. TuYV was serologically detected in 42 tested samples while all collected samples were negative for CaMV and TuMV. By using aphid transmission tests, five test plants were inoculated with two ELTSApositive TuYV samples of naturally infected mustard plants. Virus species identification was performed by amplification of a 780 bp fragment in all tested samples using the specific primers TuYVorf0F/TuYVorf0R. The RTPCR products from two isolates 88Sal (MK144816) and 98Bni (MK144817) were sequenced and compared with the GenBank sequences of TuYV. Serbian isolates showed the highest identity with Poland TuYV isolate (EU022489). Phylogenetic analysis showed that TuYV isolates from Serbia were clustered with other TuYV sequences retrieved from the GenBank.https://scindeks-clanci.ceon.rs/data/pdf/1821-3944/2019/1821-39441903082M.pdfdas-eltsamustardsphylogenetic analysisrt-pcrturnip yellows virus |
| spellingShingle | Milošević Dragana Ignjatov Maja Marjanović-Jeromela Ana Stanković Ivana Nikolić Zorica Tamindžić Gordana Krstić Branka Molecular characterization of Turnip yellows virus: A new pathogen of mustards in Serbia Ratarstvo i Povrtarstvo das-eltsa mustards phylogenetic analysis rt-pcr turnip yellows virus |
| title | Molecular characterization of Turnip yellows virus: A new pathogen of mustards in Serbia |
| title_full | Molecular characterization of Turnip yellows virus: A new pathogen of mustards in Serbia |
| title_fullStr | Molecular characterization of Turnip yellows virus: A new pathogen of mustards in Serbia |
| title_full_unstemmed | Molecular characterization of Turnip yellows virus: A new pathogen of mustards in Serbia |
| title_short | Molecular characterization of Turnip yellows virus: A new pathogen of mustards in Serbia |
| title_sort | molecular characterization of turnip yellows virus a new pathogen of mustards in serbia |
| topic | das-eltsa mustards phylogenetic analysis rt-pcr turnip yellows virus |
| url | https://scindeks-clanci.ceon.rs/data/pdf/1821-3944/2019/1821-39441903082M.pdf |
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