Validation of a multiplex amplification system of 19 autosomal STRs and 27 Y-STRs
This article describes a newly devised autosomal short tandem repeat (STR) multiplex polymerase chain reaction (PCR) system for 19 autosomal loci (D12S391, D13S317, D16S539, D18S51, D19S433, D2S1338, D21S11, D3S1358, D5S818, D6S1043, D7S820, D8S1179, CSF1PO, FGA, TH01, TPOX, vWA, Penta D and Penta E...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Oxford University Press
2020-10-01
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| Series: | Forensic Sciences Research |
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| Online Access: | http://dx.doi.org/10.1080/20961790.2019.1665158 |
| _version_ | 1797763309548601344 |
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| author | Feng Liu Fei Jia Fang Sun Bin Zhao Hongying Shen |
| author_facet | Feng Liu Fei Jia Fang Sun Bin Zhao Hongying Shen |
| author_sort | Feng Liu |
| collection | DOAJ |
| description | This article describes a newly devised autosomal short tandem repeat (STR) multiplex polymerase chain reaction (PCR) system for 19 autosomal loci (D12S391, D13S317, D16S539, D18S51, D19S433, D2S1338, D21S11, D3S1358, D5S818, D6S1043, D7S820, D8S1179, CSF1PO, FGA, TH01, TPOX, vWA, Penta D and Penta E), 27 Y-chromosome STR loci (DYS19, DYS385, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS448, DYS449, DYS456, DYS458, DYS460, DYS481, DYS518, DYS533, DYS570, DYS576, DYS635, DYS627, YGATAH4 and DYF387S1) and amelogenin with six-colour fluorescent labelling. Various parameters were evaluated, such as its accuracy, sensitivity, specificity, stability, ability to analysis of mixtures and effects of changes in the PCR-based procedures. All of the 47 selected STR loci were accurately and robustly amplified from 282 bloodstain samples. The species-specificity was high and some ability to inhibit Hematin was identified. The lowest detectable DNA amount was ≥0.125 ng. All of the male loci of the secondary component were revealed precisely when the control DNA was mixed at male/female and male/male ratios of 1:4 or more. We conclude that the present 19-plex autosomal STR and 27 Y-STR assay is both accurate and sensitive. It constitutes an additional powerful tool for forensic applications. |
| first_indexed | 2024-03-12T19:40:43Z |
| format | Article |
| id | doaj.art-ca94177abce64b4d89468868da48f479 |
| institution | Directory Open Access Journal |
| issn | 2096-1790 2471-1411 |
| language | English |
| last_indexed | 2024-03-12T19:40:43Z |
| publishDate | 2020-10-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | Forensic Sciences Research |
| spelling | doaj.art-ca94177abce64b4d89468868da48f4792023-08-02T03:54:05ZengOxford University PressForensic Sciences Research2096-17902471-14112020-10-015429229910.1080/20961790.2019.16651581665158Validation of a multiplex amplification system of 19 autosomal STRs and 27 Y-STRsFeng Liu0Fei Jia1Fang Sun2Bin Zhao3Hongying Shen4Liaoning Provincial Public Security DepartmentLiaoning Provincial Public Security DepartmentLiaoning Provincial Public Security DepartmentLiaoning Provincial Public Security DepartmentLiaoning Provincial Public Security DepartmentThis article describes a newly devised autosomal short tandem repeat (STR) multiplex polymerase chain reaction (PCR) system for 19 autosomal loci (D12S391, D13S317, D16S539, D18S51, D19S433, D2S1338, D21S11, D3S1358, D5S818, D6S1043, D7S820, D8S1179, CSF1PO, FGA, TH01, TPOX, vWA, Penta D and Penta E), 27 Y-chromosome STR loci (DYS19, DYS385, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS448, DYS449, DYS456, DYS458, DYS460, DYS481, DYS518, DYS533, DYS570, DYS576, DYS635, DYS627, YGATAH4 and DYF387S1) and amelogenin with six-colour fluorescent labelling. Various parameters were evaluated, such as its accuracy, sensitivity, specificity, stability, ability to analysis of mixtures and effects of changes in the PCR-based procedures. All of the 47 selected STR loci were accurately and robustly amplified from 282 bloodstain samples. The species-specificity was high and some ability to inhibit Hematin was identified. The lowest detectable DNA amount was ≥0.125 ng. All of the male loci of the secondary component were revealed precisely when the control DNA was mixed at male/female and male/male ratios of 1:4 or more. We conclude that the present 19-plex autosomal STR and 27 Y-STR assay is both accurate and sensitive. It constitutes an additional powerful tool for forensic applications.http://dx.doi.org/10.1080/20961790.2019.1665158forensic sciencesforensic geneticsautosomal stry-strmultiplex amplificationvalidation |
| spellingShingle | Feng Liu Fei Jia Fang Sun Bin Zhao Hongying Shen Validation of a multiplex amplification system of 19 autosomal STRs and 27 Y-STRs Forensic Sciences Research forensic sciences forensic genetics autosomal str y-str multiplex amplification validation |
| title | Validation of a multiplex amplification system of 19 autosomal STRs and 27 Y-STRs |
| title_full | Validation of a multiplex amplification system of 19 autosomal STRs and 27 Y-STRs |
| title_fullStr | Validation of a multiplex amplification system of 19 autosomal STRs and 27 Y-STRs |
| title_full_unstemmed | Validation of a multiplex amplification system of 19 autosomal STRs and 27 Y-STRs |
| title_short | Validation of a multiplex amplification system of 19 autosomal STRs and 27 Y-STRs |
| title_sort | validation of a multiplex amplification system of 19 autosomal strs and 27 y strs |
| topic | forensic sciences forensic genetics autosomal str y-str multiplex amplification validation |
| url | http://dx.doi.org/10.1080/20961790.2019.1665158 |
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