Two-Dimensional Differential Gel Electrophoresis to Identify Protein Biomarkers in Amniotic Fluid of Edwards Syndrome (Trisomy 18) Pregnancies.
BACKGROUND:Edwards syndrome (ES) is a severe chromosomal abnormality with a prevalence of about 0.8 in 10,000 infants born alive. The aims of this study were to identify candidate proteins associated with ES pregnancies from amniotic fluid supernatant (AFS) using proteomics, and to explore the role...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4713428?pdf=render |
| _version_ | 1818921852762849280 |
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| author | Te-Yao Hsu Hao Lin Hsuan-Ning Hung Kuender D Yang Chia-Yu Ou Ching-Chang Tsai Hsin-Hsin Cheng Su-Hai Chung Bi-Hua Cheng Yi-Hsun Wong An Kuo Chou Chang-Chun Hsiao |
| author_facet | Te-Yao Hsu Hao Lin Hsuan-Ning Hung Kuender D Yang Chia-Yu Ou Ching-Chang Tsai Hsin-Hsin Cheng Su-Hai Chung Bi-Hua Cheng Yi-Hsun Wong An Kuo Chou Chang-Chun Hsiao |
| author_sort | Te-Yao Hsu |
| collection | DOAJ |
| description | BACKGROUND:Edwards syndrome (ES) is a severe chromosomal abnormality with a prevalence of about 0.8 in 10,000 infants born alive. The aims of this study were to identify candidate proteins associated with ES pregnancies from amniotic fluid supernatant (AFS) using proteomics, and to explore the role of biological networks in the pathophysiology of ES. METHODS:AFS from six second trimester pregnancies with ES fetuses and six normal cases were included in this study. Fluorescence-based two-dimensional difference gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) were used for comparative proteomic analysis. The identified proteins were further validated by Western blotting and the role of biological networks was analyzed. RESULTS:Twelve protein spots were differentially expressed by more than 1.5-fold in the AFS of the ES pregnancies. MALDI-TOF/MS identified one up-regulated protein: apolipoprotein A1 (ApoA1), and four under-regulated proteins: vitamin D binding protein (VDBP), alpha-1-antitrypsin (A1AT), insulin-like growth factor-binding protein 1 (IGFBP-1), and transthyretin (TTR). Western blot and densitometric analysis of ApoA1, A1AT, IGFBP-1, and TTR confirmed the alteration of these proteins in the amniotic fluid samples. Biological network analysis revealed that the proteins of the ES AFS were involved mainly in lipid and hormone metabolism, immune response, and cardiovascular disease. CONCLUSIONS:These five proteins may be involved in the pathogenesis of ES. Further studies are needed to explore. |
| first_indexed | 2024-12-20T01:44:14Z |
| format | Article |
| id | doaj.art-ca967e12206f49cbbbcf5af55250ac24 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T01:44:14Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ca967e12206f49cbbbcf5af55250ac242022-12-21T19:57:49ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01111e014590810.1371/journal.pone.0145908Two-Dimensional Differential Gel Electrophoresis to Identify Protein Biomarkers in Amniotic Fluid of Edwards Syndrome (Trisomy 18) Pregnancies.Te-Yao HsuHao LinHsuan-Ning HungKuender D YangChia-Yu OuChing-Chang TsaiHsin-Hsin ChengSu-Hai ChungBi-Hua ChengYi-Hsun WongAn Kuo ChouChang-Chun HsiaoBACKGROUND:Edwards syndrome (ES) is a severe chromosomal abnormality with a prevalence of about 0.8 in 10,000 infants born alive. The aims of this study were to identify candidate proteins associated with ES pregnancies from amniotic fluid supernatant (AFS) using proteomics, and to explore the role of biological networks in the pathophysiology of ES. METHODS:AFS from six second trimester pregnancies with ES fetuses and six normal cases were included in this study. Fluorescence-based two-dimensional difference gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) were used for comparative proteomic analysis. The identified proteins were further validated by Western blotting and the role of biological networks was analyzed. RESULTS:Twelve protein spots were differentially expressed by more than 1.5-fold in the AFS of the ES pregnancies. MALDI-TOF/MS identified one up-regulated protein: apolipoprotein A1 (ApoA1), and four under-regulated proteins: vitamin D binding protein (VDBP), alpha-1-antitrypsin (A1AT), insulin-like growth factor-binding protein 1 (IGFBP-1), and transthyretin (TTR). Western blot and densitometric analysis of ApoA1, A1AT, IGFBP-1, and TTR confirmed the alteration of these proteins in the amniotic fluid samples. Biological network analysis revealed that the proteins of the ES AFS were involved mainly in lipid and hormone metabolism, immune response, and cardiovascular disease. CONCLUSIONS:These five proteins may be involved in the pathogenesis of ES. Further studies are needed to explore.http://europepmc.org/articles/PMC4713428?pdf=render |
| spellingShingle | Te-Yao Hsu Hao Lin Hsuan-Ning Hung Kuender D Yang Chia-Yu Ou Ching-Chang Tsai Hsin-Hsin Cheng Su-Hai Chung Bi-Hua Cheng Yi-Hsun Wong An Kuo Chou Chang-Chun Hsiao Two-Dimensional Differential Gel Electrophoresis to Identify Protein Biomarkers in Amniotic Fluid of Edwards Syndrome (Trisomy 18) Pregnancies. PLoS ONE |
| title | Two-Dimensional Differential Gel Electrophoresis to Identify Protein Biomarkers in Amniotic Fluid of Edwards Syndrome (Trisomy 18) Pregnancies. |
| title_full | Two-Dimensional Differential Gel Electrophoresis to Identify Protein Biomarkers in Amniotic Fluid of Edwards Syndrome (Trisomy 18) Pregnancies. |
| title_fullStr | Two-Dimensional Differential Gel Electrophoresis to Identify Protein Biomarkers in Amniotic Fluid of Edwards Syndrome (Trisomy 18) Pregnancies. |
| title_full_unstemmed | Two-Dimensional Differential Gel Electrophoresis to Identify Protein Biomarkers in Amniotic Fluid of Edwards Syndrome (Trisomy 18) Pregnancies. |
| title_short | Two-Dimensional Differential Gel Electrophoresis to Identify Protein Biomarkers in Amniotic Fluid of Edwards Syndrome (Trisomy 18) Pregnancies. |
| title_sort | two dimensional differential gel electrophoresis to identify protein biomarkers in amniotic fluid of edwards syndrome trisomy 18 pregnancies |
| url | http://europepmc.org/articles/PMC4713428?pdf=render |
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