Multiple dsRNases Involved in Exogenous dsRNA Degradation of Fall Armyworm Spodoptera frugiperda
RNAi is regarded as a promising technology for pest control. However, not all insects are sensitive to RNAi. Studies have confirmed that insect dsRNases are one of key factors affecting RNAi efficiency. In the current study, we identified four genes coding for dsRNases from the Spodoptera frugiperda...
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2022.850022/full |
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| author | Yang Yao Dong-Jiang Lin Xiang-Yun Cai Ran Wang You-Ming Hou Chao-Hua Hu San-Ji Gao Jin-Da Wang |
| author_facet | Yang Yao Dong-Jiang Lin Xiang-Yun Cai Ran Wang You-Ming Hou Chao-Hua Hu San-Ji Gao Jin-Da Wang |
| author_sort | Yang Yao |
| collection | DOAJ |
| description | RNAi is regarded as a promising technology for pest control. However, not all insects are sensitive to RNAi. Studies have confirmed that insect dsRNases are one of key factors affecting RNAi efficiency. In the current study, we identified four genes coding for dsRNases from the Spodoptera frugiperda genome. Spatial and temporal expression analysis showed that those dsRNases were highly expressed in the midgut and old larvae. Then a delivery method was applied for inducing efficient RNAi based on dsRNA encapsulated by liposome. Furthermore, we assessed degradation efficiency by incubation with dsRNA with gut juice or hemocoel to characterize potential roles of different SfdsRNases after suppression of SfdsRNase. The result showed that interferenced with any sfdsRNase reduced the degradation of exogenous dsRNA in midgut, interfered with sfdsRNase1 and sfdsRNase3 slowed down the degradation of exogenous dsRNA in hemolymph. Our data suggest the evolutionary expansion and multiple high activity dsRNase genes would take part in the RNAi obstinate in S. frugiperda, besides we also provide an efficient RNAi method for better use of RNAi in S. frugiperda. |
| first_indexed | 2024-12-10T05:21:38Z |
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| id | doaj.art-ca9a256f2cf54af5a5ae5c796cad568d |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-10T05:21:38Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Physiology |
| spelling | doaj.art-ca9a256f2cf54af5a5ae5c796cad568d2022-12-22T02:00:47ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2022-05-011310.3389/fphys.2022.850022850022Multiple dsRNases Involved in Exogenous dsRNA Degradation of Fall Armyworm Spodoptera frugiperdaYang Yao0Dong-Jiang Lin1Xiang-Yun Cai2Ran Wang3You-Ming Hou4Chao-Hua Hu5San-Ji Gao6Jin-Da Wang7National Engineering Research Center of Sugarcane, Fujian Agricultural and Forestry University, Fuzhou, ChinaNational Engineering Research Center of Sugarcane, Fujian Agricultural and Forestry University, Fuzhou, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Ministry of Education, College of Plant Protection, Fujian Agricultural and Forestry University, Fuzhou, ChinaInstitute of Plant Protection, Beijing Academy of Agriculture and Forestry, Beijing, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Ministry of Education, College of Plant Protection, Fujian Agricultural and Forestry University, Fuzhou, ChinaNational Engineering Research Center of Sugarcane, Fujian Agricultural and Forestry University, Fuzhou, ChinaNational Engineering Research Center of Sugarcane, Fujian Agricultural and Forestry University, Fuzhou, ChinaNational Engineering Research Center of Sugarcane, Fujian Agricultural and Forestry University, Fuzhou, ChinaRNAi is regarded as a promising technology for pest control. However, not all insects are sensitive to RNAi. Studies have confirmed that insect dsRNases are one of key factors affecting RNAi efficiency. In the current study, we identified four genes coding for dsRNases from the Spodoptera frugiperda genome. Spatial and temporal expression analysis showed that those dsRNases were highly expressed in the midgut and old larvae. Then a delivery method was applied for inducing efficient RNAi based on dsRNA encapsulated by liposome. Furthermore, we assessed degradation efficiency by incubation with dsRNA with gut juice or hemocoel to characterize potential roles of different SfdsRNases after suppression of SfdsRNase. The result showed that interferenced with any sfdsRNase reduced the degradation of exogenous dsRNA in midgut, interfered with sfdsRNase1 and sfdsRNase3 slowed down the degradation of exogenous dsRNA in hemolymph. Our data suggest the evolutionary expansion and multiple high activity dsRNase genes would take part in the RNAi obstinate in S. frugiperda, besides we also provide an efficient RNAi method for better use of RNAi in S. frugiperda.https://www.frontiersin.org/articles/10.3389/fphys.2022.850022/fullRNA inferencedsRNaseefficiencyliposomeSpodoptera frugiperda |
| spellingShingle | Yang Yao Dong-Jiang Lin Xiang-Yun Cai Ran Wang You-Ming Hou Chao-Hua Hu San-Ji Gao Jin-Da Wang Multiple dsRNases Involved in Exogenous dsRNA Degradation of Fall Armyworm Spodoptera frugiperda Frontiers in Physiology RNA inference dsRNase efficiency liposome Spodoptera frugiperda |
| title | Multiple dsRNases Involved in Exogenous dsRNA Degradation of Fall Armyworm Spodoptera frugiperda |
| title_full | Multiple dsRNases Involved in Exogenous dsRNA Degradation of Fall Armyworm Spodoptera frugiperda |
| title_fullStr | Multiple dsRNases Involved in Exogenous dsRNA Degradation of Fall Armyworm Spodoptera frugiperda |
| title_full_unstemmed | Multiple dsRNases Involved in Exogenous dsRNA Degradation of Fall Armyworm Spodoptera frugiperda |
| title_short | Multiple dsRNases Involved in Exogenous dsRNA Degradation of Fall Armyworm Spodoptera frugiperda |
| title_sort | multiple dsrnases involved in exogenous dsrna degradation of fall armyworm spodoptera frugiperda |
| topic | RNA inference dsRNase efficiency liposome Spodoptera frugiperda |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2022.850022/full |
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