Diagnostic value of cardiac miR-126-5p, miR-134-5p, and miR-499a-5p in coronary artery disease-induced sudden cardiac death
BackgroundThe identification of coronary artery disease-induced sudden cardiac death (CAD-SCD) has always been a medical challenge. MicroRNAs (miRNAs) played vital roles in pathogenesis processes and served as potential biomarkers for cardiovascular and many other diseases. The aim of this study was...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-08-01
|
| Series: | Frontiers in Cardiovascular Medicine |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcvm.2022.944317/full |
| _version_ | 1817997818539802624 |
|---|---|
| author | Linfeng Li Xiangwang He Min Liu Libing Yun Bin Cong Bin Cong |
| author_facet | Linfeng Li Xiangwang He Min Liu Libing Yun Bin Cong Bin Cong |
| author_sort | Linfeng Li |
| collection | DOAJ |
| description | BackgroundThe identification of coronary artery disease-induced sudden cardiac death (CAD-SCD) has always been a medical challenge. MicroRNAs (miRNAs) played vital roles in pathogenesis processes and served as potential biomarkers for cardiovascular and many other diseases. The aim of this study was to investigate the diagnostic value of the specific miRNAs for CAD-SCD.MethodsA total of 30 autopsy-verified CAD-SCD victims were selected, including 18 individuals who experienced more than once asymptomatic myocardial ischemia (CAD-activated SCD) and 12 victims without prominent pathological features of insufficient blood supply (CAD-silent SCD). Meanwhile, 30 traumatic victims were enrolled as controls. Systematic postmortem examinations were performed in all study population. The expressions of cardiac miR-126-5p, miR-134-5p, and miR-499a-5p were analyzed by real-time quantitative polymerase chain reaction (RT-qPCR).ResultsRT-qPCR showed significant downregulations of miR-126-5p and miR-499a-5p in CAD-SCD victims, with no obvious difference in miR-134-5p. Receiver-operating characteristic analysis revealed the diagnostic performance of miR-126-5p (areas under the curve [AUC] = 0.76) and validated miR-499a-5p (AUC = 0.82) as a sensitive marker. Additionally, the decreased expression of the two specific cardio-miRNAs was detected for discriminating CAD-silent SCD and CAD-activated SCD. Compared with the limited diagnostic value of single miR-126-5p and miR-499a-5p, their combination could achieve better discriminative capacity (AUC = 0.82, sensitivity = 91.7%, specificity = 77.8%).ConclusionCardiac miR-126-5p and miR-499a-5p presented good diagnostic abilities for CAD-SCD, and their combination could help evaluate CAD condition. These targeted miRNAs as novel biomarkers are expected to be useful to discriminate the detailed causes in real SCD cases. |
| first_indexed | 2024-04-14T02:43:54Z |
| format | Article |
| id | doaj.art-ca9b1fe9612d4133a665caa7a0b91690 |
| institution | Directory Open Access Journal |
| issn | 2297-055X |
| language | English |
| last_indexed | 2024-04-14T02:43:54Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cardiovascular Medicine |
| spelling | doaj.art-ca9b1fe9612d4133a665caa7a0b916902022-12-22T02:16:39ZengFrontiers Media S.A.Frontiers in Cardiovascular Medicine2297-055X2022-08-01910.3389/fcvm.2022.944317944317Diagnostic value of cardiac miR-126-5p, miR-134-5p, and miR-499a-5p in coronary artery disease-induced sudden cardiac deathLinfeng Li0Xiangwang He1Min Liu2Libing Yun3Bin Cong4Bin Cong5Department of Forensic Pathology, West China School of Basic Medical Sciences and Forensic Science, Sichuan University, Chengdu, ChinaDepartment of Forensic Pathology, West China School of Basic Medical Sciences and Forensic Science, Sichuan University, Chengdu, ChinaDepartment of Forensic Pathology, West China School of Basic Medical Sciences and Forensic Science, Sichuan University, Chengdu, ChinaDepartment of Forensic Pathology, West China School of Basic Medical Sciences and Forensic Science, Sichuan University, Chengdu, ChinaDepartment of Forensic Pathology, West China School of Basic Medical Sciences and Forensic Science, Sichuan University, Chengdu, ChinaDepartment of Forensic Medicine, Hebei Medical University, Shijiazhuang, ChinaBackgroundThe identification of coronary artery disease-induced sudden cardiac death (CAD-SCD) has always been a medical challenge. MicroRNAs (miRNAs) played vital roles in pathogenesis processes and served as potential biomarkers for cardiovascular and many other diseases. The aim of this study was to investigate the diagnostic value of the specific miRNAs for CAD-SCD.MethodsA total of 30 autopsy-verified CAD-SCD victims were selected, including 18 individuals who experienced more than once asymptomatic myocardial ischemia (CAD-activated SCD) and 12 victims without prominent pathological features of insufficient blood supply (CAD-silent SCD). Meanwhile, 30 traumatic victims were enrolled as controls. Systematic postmortem examinations were performed in all study population. The expressions of cardiac miR-126-5p, miR-134-5p, and miR-499a-5p were analyzed by real-time quantitative polymerase chain reaction (RT-qPCR).ResultsRT-qPCR showed significant downregulations of miR-126-5p and miR-499a-5p in CAD-SCD victims, with no obvious difference in miR-134-5p. Receiver-operating characteristic analysis revealed the diagnostic performance of miR-126-5p (areas under the curve [AUC] = 0.76) and validated miR-499a-5p (AUC = 0.82) as a sensitive marker. Additionally, the decreased expression of the two specific cardio-miRNAs was detected for discriminating CAD-silent SCD and CAD-activated SCD. Compared with the limited diagnostic value of single miR-126-5p and miR-499a-5p, their combination could achieve better discriminative capacity (AUC = 0.82, sensitivity = 91.7%, specificity = 77.8%).ConclusionCardiac miR-126-5p and miR-499a-5p presented good diagnostic abilities for CAD-SCD, and their combination could help evaluate CAD condition. These targeted miRNAs as novel biomarkers are expected to be useful to discriminate the detailed causes in real SCD cases.https://www.frontiersin.org/articles/10.3389/fcvm.2022.944317/fullcoronary artery diseasesudden cardiac deathmicroRNAmyocardial hypertrophymyocardial fibrosis |
| spellingShingle | Linfeng Li Xiangwang He Min Liu Libing Yun Bin Cong Bin Cong Diagnostic value of cardiac miR-126-5p, miR-134-5p, and miR-499a-5p in coronary artery disease-induced sudden cardiac death Frontiers in Cardiovascular Medicine coronary artery disease sudden cardiac death microRNA myocardial hypertrophy myocardial fibrosis |
| title | Diagnostic value of cardiac miR-126-5p, miR-134-5p, and miR-499a-5p in coronary artery disease-induced sudden cardiac death |
| title_full | Diagnostic value of cardiac miR-126-5p, miR-134-5p, and miR-499a-5p in coronary artery disease-induced sudden cardiac death |
| title_fullStr | Diagnostic value of cardiac miR-126-5p, miR-134-5p, and miR-499a-5p in coronary artery disease-induced sudden cardiac death |
| title_full_unstemmed | Diagnostic value of cardiac miR-126-5p, miR-134-5p, and miR-499a-5p in coronary artery disease-induced sudden cardiac death |
| title_short | Diagnostic value of cardiac miR-126-5p, miR-134-5p, and miR-499a-5p in coronary artery disease-induced sudden cardiac death |
| title_sort | diagnostic value of cardiac mir 126 5p mir 134 5p and mir 499a 5p in coronary artery disease induced sudden cardiac death |
| topic | coronary artery disease sudden cardiac death microRNA myocardial hypertrophy myocardial fibrosis |
| url | https://www.frontiersin.org/articles/10.3389/fcvm.2022.944317/full |
| work_keys_str_mv | AT linfengli diagnosticvalueofcardiacmir1265pmir1345pandmir499a5pincoronaryarterydiseaseinducedsuddencardiacdeath AT xiangwanghe diagnosticvalueofcardiacmir1265pmir1345pandmir499a5pincoronaryarterydiseaseinducedsuddencardiacdeath AT minliu diagnosticvalueofcardiacmir1265pmir1345pandmir499a5pincoronaryarterydiseaseinducedsuddencardiacdeath AT libingyun diagnosticvalueofcardiacmir1265pmir1345pandmir499a5pincoronaryarterydiseaseinducedsuddencardiacdeath AT bincong diagnosticvalueofcardiacmir1265pmir1345pandmir499a5pincoronaryarterydiseaseinducedsuddencardiacdeath AT bincong diagnosticvalueofcardiacmir1265pmir1345pandmir499a5pincoronaryarterydiseaseinducedsuddencardiacdeath |