Analyses of variant human papillomavirus type-16 E5 proteins for their ability to induce mitogenesis of murine fibroblasts
<p>Abstract</p> <p>Background</p> <p>Human papillomavirus type 16 (HPV-16) E5 protein co-operates with epidermal growth factor to stimulate mitogenesis of murine fibroblasts. Currently, little is known about which viral amino acids are involved in this process. Using se...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2006-08-01
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| Series: | Cancer Cell International |
| Online Access: | http://www.cancerci.com/content/6/1/19 |
| _version_ | 1818067852456886272 |
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| author | Cason John Kell Barbara Mant Christine A Nath Rahul Bible Jon M |
| author_facet | Cason John Kell Barbara Mant Christine A Nath Rahul Bible Jon M |
| author_sort | Cason John |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Human papillomavirus type 16 (HPV-16) E5 protein co-operates with epidermal growth factor to stimulate mitogenesis of murine fibroblasts. Currently, little is known about which viral amino acids are involved in this process. Using sequence variants of HPV-16 E5 we have investigated their effects upon E5 transcription, cell-cycling and cell-growth of murine fibroblasts.</p> <p>Results</p> <p>We demonstrate that: (i) introduction of Thr<sup>64 </sup>into the reference E5 sequence of HPV-16 abrogates mitogenic activity: both were poorly transcribed in NIH-3T3 cells; (ii) substitution of Leu<sup>44</sup>Val<sup>65 </sup>or, Thr<sup>37</sup>Leu<sup>44</sup>Val<sup>65 </sup>into the HPV-16 E5 reference backbone resulted in high transcription in NIH-3T3 cells, enhanced cell-cycle progression and high cell-growth; and, (iii) inclusion of Tyr<sup>8 </sup>into the Leu<sup>44</sup>Val<sup>65 </sup>backbone inhibited E5 induced cell-growth and repression of p21 expression, despite high transcription levels.</p> <p>Conclusion</p> <p>The effects of HPV-16 E5 variants upon mitosis help to explain why Leu<sup>44</sup>Val<sup>65 </sup>HPV-16 E5 variants are most prevalent in 'wild' pathogenic viral populations in the UK.</p> |
| first_indexed | 2024-12-10T15:30:16Z |
| format | Article |
| id | doaj.art-ca9cbba4e15e4040bca5eba2fd1b3937 |
| institution | Directory Open Access Journal |
| issn | 1475-2867 |
| language | English |
| last_indexed | 2024-12-10T15:30:16Z |
| publishDate | 2006-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Cell International |
| spelling | doaj.art-ca9cbba4e15e4040bca5eba2fd1b39372022-12-22T01:43:25ZengBMCCancer Cell International1475-28672006-08-01611910.1186/1475-2867-6-19Analyses of variant human papillomavirus type-16 E5 proteins for their ability to induce mitogenesis of murine fibroblastsCason JohnKell BarbaraMant Christine ANath RahulBible Jon M<p>Abstract</p> <p>Background</p> <p>Human papillomavirus type 16 (HPV-16) E5 protein co-operates with epidermal growth factor to stimulate mitogenesis of murine fibroblasts. Currently, little is known about which viral amino acids are involved in this process. Using sequence variants of HPV-16 E5 we have investigated their effects upon E5 transcription, cell-cycling and cell-growth of murine fibroblasts.</p> <p>Results</p> <p>We demonstrate that: (i) introduction of Thr<sup>64 </sup>into the reference E5 sequence of HPV-16 abrogates mitogenic activity: both were poorly transcribed in NIH-3T3 cells; (ii) substitution of Leu<sup>44</sup>Val<sup>65 </sup>or, Thr<sup>37</sup>Leu<sup>44</sup>Val<sup>65 </sup>into the HPV-16 E5 reference backbone resulted in high transcription in NIH-3T3 cells, enhanced cell-cycle progression and high cell-growth; and, (iii) inclusion of Tyr<sup>8 </sup>into the Leu<sup>44</sup>Val<sup>65 </sup>backbone inhibited E5 induced cell-growth and repression of p21 expression, despite high transcription levels.</p> <p>Conclusion</p> <p>The effects of HPV-16 E5 variants upon mitosis help to explain why Leu<sup>44</sup>Val<sup>65 </sup>HPV-16 E5 variants are most prevalent in 'wild' pathogenic viral populations in the UK.</p>http://www.cancerci.com/content/6/1/19 |
| spellingShingle | Cason John Kell Barbara Mant Christine A Nath Rahul Bible Jon M Analyses of variant human papillomavirus type-16 E5 proteins for their ability to induce mitogenesis of murine fibroblasts Cancer Cell International |
| title | Analyses of variant human papillomavirus type-16 E5 proteins for their ability to induce mitogenesis of murine fibroblasts |
| title_full | Analyses of variant human papillomavirus type-16 E5 proteins for their ability to induce mitogenesis of murine fibroblasts |
| title_fullStr | Analyses of variant human papillomavirus type-16 E5 proteins for their ability to induce mitogenesis of murine fibroblasts |
| title_full_unstemmed | Analyses of variant human papillomavirus type-16 E5 proteins for their ability to induce mitogenesis of murine fibroblasts |
| title_short | Analyses of variant human papillomavirus type-16 E5 proteins for their ability to induce mitogenesis of murine fibroblasts |
| title_sort | analyses of variant human papillomavirus type 16 e5 proteins for their ability to induce mitogenesis of murine fibroblasts |
| url | http://www.cancerci.com/content/6/1/19 |
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