PCE3 Plays a Role in the Reproduction of Male <i>Nilaparvata lugens</i>
<i>Nilaparvata lugens</i> proclotting enzymes (<i>Nl</i>PCEs) belong to the clip domain serine protease (clip-SP) family, which is a characteristic protease family in arthropods. <i>NlPCE3</i> was previously reported to regulate egg production and development in f...
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MDPI AG
2021-01-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/12/2/114 |
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| author | Rong-er Zheng Jinliang Ji Jiamin Wu Ruijuan Zhang Yabin Li Xiaoping Yu Yipeng Xu |
| author_facet | Rong-er Zheng Jinliang Ji Jiamin Wu Ruijuan Zhang Yabin Li Xiaoping Yu Yipeng Xu |
| author_sort | Rong-er Zheng |
| collection | DOAJ |
| description | <i>Nilaparvata lugens</i> proclotting enzymes (<i>Nl</i>PCEs) belong to the clip domain serine protease (clip-SP) family, which is a characteristic protease family in arthropods. <i>NlPCE3</i> was previously reported to regulate egg production and development in female <i>N. lugens</i>, but its role in male <i>N. lugens</i> is unclear. In the present study, qPCR analysis showed that <i>NlPCE3</i> was expressed in three different tissues (gut, testis and fat body). RNAi revealed that ds<i>NlPCE3</i> injection made the male vas deferens thinner and reduced the oviposition level of the females that mated with ds<i>NlPCE3</i>-treated males, causing eggs not to hatch. Furthermore, immunofluorescence staining showed that <i>Nl</i>PCE3 was widely expressed in the male internal genitalia. However, after ds<i>NlPCE3</i> injection, expression of <i>Nl</i>PCE3 was diffuse in the male internal genitalia, whose peripheral cells seemed degraded. Overall, these results indicate that <i>NlPCE3</i> is important for reproduction in male <i>N. lugens</i>. |
| first_indexed | 2024-03-09T03:26:46Z |
| format | Article |
| id | doaj.art-caa246bcf9984fde8d064bfbef5c98ac |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-09T03:26:46Z |
| publishDate | 2021-01-01 |
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| series | Insects |
| spelling | doaj.art-caa246bcf9984fde8d064bfbef5c98ac2023-12-03T15:01:18ZengMDPI AGInsects2075-44502021-01-0112211410.3390/insects12020114PCE3 Plays a Role in the Reproduction of Male <i>Nilaparvata lugens</i>Rong-er Zheng0Jinliang Ji1Jiamin Wu2Ruijuan Zhang3Yabin Li4Xiaoping Yu5Yipeng Xu6Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, China Jiliang University, Hangzhou 310018, ChinaZhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, China Jiliang University, Hangzhou 310018, ChinaZhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, China Jiliang University, Hangzhou 310018, ChinaZhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, China Jiliang University, Hangzhou 310018, ChinaZhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, China Jiliang University, Hangzhou 310018, ChinaZhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, China Jiliang University, Hangzhou 310018, ChinaZhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, China Jiliang University, Hangzhou 310018, China<i>Nilaparvata lugens</i> proclotting enzymes (<i>Nl</i>PCEs) belong to the clip domain serine protease (clip-SP) family, which is a characteristic protease family in arthropods. <i>NlPCE3</i> was previously reported to regulate egg production and development in female <i>N. lugens</i>, but its role in male <i>N. lugens</i> is unclear. In the present study, qPCR analysis showed that <i>NlPCE3</i> was expressed in three different tissues (gut, testis and fat body). RNAi revealed that ds<i>NlPCE3</i> injection made the male vas deferens thinner and reduced the oviposition level of the females that mated with ds<i>NlPCE3</i>-treated males, causing eggs not to hatch. Furthermore, immunofluorescence staining showed that <i>Nl</i>PCE3 was widely expressed in the male internal genitalia. However, after ds<i>NlPCE3</i> injection, expression of <i>Nl</i>PCE3 was diffuse in the male internal genitalia, whose peripheral cells seemed degraded. Overall, these results indicate that <i>NlPCE3</i> is important for reproduction in male <i>N. lugens</i>.https://www.mdpi.com/2075-4450/12/2/114<i>Nilaparvata lugens</i>proclotting enzymeRNAireproduction |
| spellingShingle | Rong-er Zheng Jinliang Ji Jiamin Wu Ruijuan Zhang Yabin Li Xiaoping Yu Yipeng Xu PCE3 Plays a Role in the Reproduction of Male <i>Nilaparvata lugens</i> Insects <i>Nilaparvata lugens</i> proclotting enzyme RNAi reproduction |
| title | PCE3 Plays a Role in the Reproduction of Male <i>Nilaparvata lugens</i> |
| title_full | PCE3 Plays a Role in the Reproduction of Male <i>Nilaparvata lugens</i> |
| title_fullStr | PCE3 Plays a Role in the Reproduction of Male <i>Nilaparvata lugens</i> |
| title_full_unstemmed | PCE3 Plays a Role in the Reproduction of Male <i>Nilaparvata lugens</i> |
| title_short | PCE3 Plays a Role in the Reproduction of Male <i>Nilaparvata lugens</i> |
| title_sort | pce3 plays a role in the reproduction of male i nilaparvata lugens i |
| topic | <i>Nilaparvata lugens</i> proclotting enzyme RNAi reproduction |
| url | https://www.mdpi.com/2075-4450/12/2/114 |
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