Prevention of Cyclophosphamide-Induced Immunosuppression in Mice With Traditional Chinese Medicine Xuanfei Baidu Decoction
Background and aims: Xuanfei Baidu decoction (XFBD), a traditional Chinese medicine formulation, was designed and successfully applied for COVID-19 disease treatment in China, while the mechanism is still not clear.Methods: To evaluate the protective effect of XFBD on immunosuppression in cyclophosp...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-10-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2021.730567/full |
| _version_ | 1819005251905126400 |
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| author | Huimin Yan Huimin Yan Jia Lu Jia Lu Jiabao Wang Jiabao Wang Jiabao Wang Lu Chen Lu Chen Lu Chen Yu Wang Yu Wang Yu Wang Lin Li Lin Li Lin Li Lin Miao Lin Miao Lin Miao Han Zhang Han Zhang Han Zhang |
| author_facet | Huimin Yan Huimin Yan Jia Lu Jia Lu Jiabao Wang Jiabao Wang Jiabao Wang Lu Chen Lu Chen Lu Chen Yu Wang Yu Wang Yu Wang Lin Li Lin Li Lin Li Lin Miao Lin Miao Lin Miao Han Zhang Han Zhang Han Zhang |
| author_sort | Huimin Yan |
| collection | DOAJ |
| description | Background and aims: Xuanfei Baidu decoction (XFBD), a traditional Chinese medicine formulation, was designed and successfully applied for COVID-19 disease treatment in China, while the mechanism is still not clear.Methods: To evaluate the protective effect of XFBD on immunosuppression in cyclophosphamide (CY)-treated mice, XFBD was orally administrated, the body weight was measured, and the immune organ index was calculated. HE staining was performed to analyze the pathological structures of the liver, spleen, and thymus. The levels of cytokines and immunoglobulin in the serum and spleen were evaluated by ELISA and RT-PCR. Splenic lymphocytes were isolated, and LPS-stimulated cell proliferation and the number of CD4+ and CD8+ T lymphocytes were evaluated.Results: XFBD significantly suppressed body weight loss and increased the indices of spleen and thymus. The pathological alteration was much improved after XFBD administration. The reductions of TNF-α, IFN-γ, IgG, and IgM levels in serum and IL-2, IL-4, and IL-6 expressions in the spleen were all significantly alleviated by XFBD. Splenic lymphocyte proliferation in response to LPS was further enhanced after treatment with XFBD. The reduction of CD4+ and CD8+ T lymphocytes in CY-treated mice was also highly increased in XFBD groups.Conclusion: Our findings suggested that XFBD played a crucial role in protection against immunosuppression in CY-treated mice and could be a potential candidate for immune modification and therapy. |
| first_indexed | 2024-12-20T23:49:50Z |
| format | Article |
| id | doaj.art-caa414cdbc084bb2aef0b0b7716e1aef |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-20T23:49:50Z |
| publishDate | 2021-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-caa414cdbc084bb2aef0b0b7716e1aef2022-12-21T19:22:52ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122021-10-011210.3389/fphar.2021.730567730567Prevention of Cyclophosphamide-Induced Immunosuppression in Mice With Traditional Chinese Medicine Xuanfei Baidu DecoctionHuimin Yan0Huimin Yan1Jia Lu2Jia Lu3Jiabao Wang4Jiabao Wang5Jiabao Wang6Lu Chen7Lu Chen8Lu Chen9Yu Wang10Yu Wang11Yu Wang12Lin Li13Lin Li14Lin Li15Lin Miao16Lin Miao17Lin Miao18Han Zhang19Han Zhang20Han Zhang21State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaInstitute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaInstitute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaKey Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaState Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaInstitute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaKey Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaState Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaInstitute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaKey Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaState Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaInstitute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaKey Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaState Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaInstitute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaKey Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaState Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaInstitute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaKey Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaState Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaInstitute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaKey Laboratory of Pharmacology of Traditional Chinese Medical Formulae, Ministry of Education, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaBackground and aims: Xuanfei Baidu decoction (XFBD), a traditional Chinese medicine formulation, was designed and successfully applied for COVID-19 disease treatment in China, while the mechanism is still not clear.Methods: To evaluate the protective effect of XFBD on immunosuppression in cyclophosphamide (CY)-treated mice, XFBD was orally administrated, the body weight was measured, and the immune organ index was calculated. HE staining was performed to analyze the pathological structures of the liver, spleen, and thymus. The levels of cytokines and immunoglobulin in the serum and spleen were evaluated by ELISA and RT-PCR. Splenic lymphocytes were isolated, and LPS-stimulated cell proliferation and the number of CD4+ and CD8+ T lymphocytes were evaluated.Results: XFBD significantly suppressed body weight loss and increased the indices of spleen and thymus. The pathological alteration was much improved after XFBD administration. The reductions of TNF-α, IFN-γ, IgG, and IgM levels in serum and IL-2, IL-4, and IL-6 expressions in the spleen were all significantly alleviated by XFBD. Splenic lymphocyte proliferation in response to LPS was further enhanced after treatment with XFBD. The reduction of CD4+ and CD8+ T lymphocytes in CY-treated mice was also highly increased in XFBD groups.Conclusion: Our findings suggested that XFBD played a crucial role in protection against immunosuppression in CY-treated mice and could be a potential candidate for immune modification and therapy.https://www.frontiersin.org/articles/10.3389/fphar.2021.730567/fullXuanfei Baidu decoctionimmunosuppressionimmune modificationcyclophosphamidetraditional Chinese medicine |
| spellingShingle | Huimin Yan Huimin Yan Jia Lu Jia Lu Jiabao Wang Jiabao Wang Jiabao Wang Lu Chen Lu Chen Lu Chen Yu Wang Yu Wang Yu Wang Lin Li Lin Li Lin Li Lin Miao Lin Miao Lin Miao Han Zhang Han Zhang Han Zhang Prevention of Cyclophosphamide-Induced Immunosuppression in Mice With Traditional Chinese Medicine Xuanfei Baidu Decoction Frontiers in Pharmacology Xuanfei Baidu decoction immunosuppression immune modification cyclophosphamide traditional Chinese medicine |
| title | Prevention of Cyclophosphamide-Induced Immunosuppression in Mice With Traditional Chinese Medicine Xuanfei Baidu Decoction |
| title_full | Prevention of Cyclophosphamide-Induced Immunosuppression in Mice With Traditional Chinese Medicine Xuanfei Baidu Decoction |
| title_fullStr | Prevention of Cyclophosphamide-Induced Immunosuppression in Mice With Traditional Chinese Medicine Xuanfei Baidu Decoction |
| title_full_unstemmed | Prevention of Cyclophosphamide-Induced Immunosuppression in Mice With Traditional Chinese Medicine Xuanfei Baidu Decoction |
| title_short | Prevention of Cyclophosphamide-Induced Immunosuppression in Mice With Traditional Chinese Medicine Xuanfei Baidu Decoction |
| title_sort | prevention of cyclophosphamide induced immunosuppression in mice with traditional chinese medicine xuanfei baidu decoction |
| topic | Xuanfei Baidu decoction immunosuppression immune modification cyclophosphamide traditional Chinese medicine |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2021.730567/full |
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