Potential Role of Photosynthesis in the Regulation of Reactive Oxygen Species and Defence Responses to <i>Blumeria graminis</i> f. sp. <i>tritici</i> in Wheat

Photosynthesis is not only a primary generator of reactive oxygen species (ROS) but also a component of plant defence. To determine the relationships among photosynthesis, ROS, and defence responses to powdery mildew in wheat, we compared the responses of the <i>Pm40</i>-expressing wheat...

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Main Authors: Yuting Hu, Shengfu Zhong, Min Zhang, Yinping Liang, Guoshu Gong, Xiaoli Chang, Feiquan Tan, Huai Yang, Xiaoyan Qiu, Liya Luo, Peigao Luo
Format: Article
Language:English
Published: MDPI AG 2020-08-01
Series:International Journal of Molecular Sciences
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Online Access:https://www.mdpi.com/1422-0067/21/16/5767
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author Yuting Hu
Shengfu Zhong
Min Zhang
Yinping Liang
Guoshu Gong
Xiaoli Chang
Feiquan Tan
Huai Yang
Xiaoyan Qiu
Liya Luo
Peigao Luo
author_facet Yuting Hu
Shengfu Zhong
Min Zhang
Yinping Liang
Guoshu Gong
Xiaoli Chang
Feiquan Tan
Huai Yang
Xiaoyan Qiu
Liya Luo
Peigao Luo
author_sort Yuting Hu
collection DOAJ
description Photosynthesis is not only a primary generator of reactive oxygen species (ROS) but also a component of plant defence. To determine the relationships among photosynthesis, ROS, and defence responses to powdery mildew in wheat, we compared the responses of the <i>Pm40</i>-expressing wheat line L658 and its susceptible sister line L958 at 0, 6, 12, 24, 48, and 72 h post-inoculation (hpi) with powdery mildew via analyses of transcriptomes, cytology, antioxidant activities, photosynthesis, and chlorophyll fluorescence parameters. The results showed that H<sub>2</sub>O<sub>2</sub> accumulation in L658 was significantly greater than that in L958 at 6 and 48 hpi, and the enzymes activity and transcripts expression of peroxidase and catalase were suppressed in L658 compared with L958. In addition, the inhibition of photosynthesis in L658 paralleled the global downregulation of photosynthesis-related genes. Furthermore, the expression of the salicylic acid-related genes non-expressor of pathogenesis related genes 1 (<i>NPR1</i>), pathogenesis-related 1 <i>(PR1)</i>, and pathogenesis-related 5 (<i>PR5</i>) was upregulated, while the expression of jasmonic acid- and ethylene-related genes was inhibited in L658 compared with L958. In conclusion, the downregulation of photosynthesis-related genes likely led to a decline in photosynthesis, which may be combined with the inhibition of peroxidase (POD) and catalase (CAT) to generate two stages of H<sub>2</sub>O<sub>2</sub> accumulation. The high level of H<sub>2</sub>O<sub>2,</sub> salicylic acid and <i>PR1</i> and <i>PR5</i> in L658 possible initiated the hypersensitive response.
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spelling doaj.art-caa635cf95804d1dba50910fa48cf0df2023-11-20T09:49:43ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-08-012116576710.3390/ijms21165767Potential Role of Photosynthesis in the Regulation of Reactive Oxygen Species and Defence Responses to <i>Blumeria graminis</i> f. sp. <i>tritici</i> in WheatYuting Hu0Shengfu Zhong1Min Zhang2Yinping Liang3Guoshu Gong4Xiaoli Chang5Feiquan Tan6Huai Yang7Xiaoyan Qiu8Liya Luo9Peigao Luo10Provincial Key Laboratory of Plant Breeding and Genetics, College of Agronomy, Sichuan Agricultural University, Chengdu 611130, Sichuan, ChinaProvincial Key Laboratory of Plant Breeding and Genetics, College of Agronomy, Sichuan Agricultural University, Chengdu 611130, Sichuan, ChinaCollege of Agronomy & Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu 611130, Sichuan, ChinaProvincial Key Laboratory of Plant Breeding and Genetics, College of Agronomy, Sichuan Agricultural University, Chengdu 611130, Sichuan, ChinaProvincial Key Laboratory of Plant Breeding and Genetics, College of Agronomy, Sichuan Agricultural University, Chengdu 611130, Sichuan, ChinaProvincial Key Laboratory of Plant Breeding and Genetics, College of Agronomy, Sichuan Agricultural University, Chengdu 611130, Sichuan, ChinaProvincial Key Laboratory of Plant Breeding and Genetics, College of Agronomy, Sichuan Agricultural University, Chengdu 611130, Sichuan, ChinaProvincial Key Laboratory of Plant Breeding and Genetics, College of Agronomy, Sichuan Agricultural University, Chengdu 611130, Sichuan, ChinaCollege of Agronomy & Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu 611130, Sichuan, ChinaCollege of Agronomy & Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu 611130, Sichuan, ChinaProvincial Key Laboratory of Plant Breeding and Genetics, College of Agronomy, Sichuan Agricultural University, Chengdu 611130, Sichuan, ChinaPhotosynthesis is not only a primary generator of reactive oxygen species (ROS) but also a component of plant defence. To determine the relationships among photosynthesis, ROS, and defence responses to powdery mildew in wheat, we compared the responses of the <i>Pm40</i>-expressing wheat line L658 and its susceptible sister line L958 at 0, 6, 12, 24, 48, and 72 h post-inoculation (hpi) with powdery mildew via analyses of transcriptomes, cytology, antioxidant activities, photosynthesis, and chlorophyll fluorescence parameters. The results showed that H<sub>2</sub>O<sub>2</sub> accumulation in L658 was significantly greater than that in L958 at 6 and 48 hpi, and the enzymes activity and transcripts expression of peroxidase and catalase were suppressed in L658 compared with L958. In addition, the inhibition of photosynthesis in L658 paralleled the global downregulation of photosynthesis-related genes. Furthermore, the expression of the salicylic acid-related genes non-expressor of pathogenesis related genes 1 (<i>NPR1</i>), pathogenesis-related 1 <i>(PR1)</i>, and pathogenesis-related 5 (<i>PR5</i>) was upregulated, while the expression of jasmonic acid- and ethylene-related genes was inhibited in L658 compared with L958. In conclusion, the downregulation of photosynthesis-related genes likely led to a decline in photosynthesis, which may be combined with the inhibition of peroxidase (POD) and catalase (CAT) to generate two stages of H<sub>2</sub>O<sub>2</sub> accumulation. The high level of H<sub>2</sub>O<sub>2,</sub> salicylic acid and <i>PR1</i> and <i>PR5</i> in L658 possible initiated the hypersensitive response.https://www.mdpi.com/1422-0067/21/16/5767wheat powdery mildewphotosynthesisreactive oxygen speciesplant defencesalicylic acidtranscriptomics
spellingShingle Yuting Hu
Shengfu Zhong
Min Zhang
Yinping Liang
Guoshu Gong
Xiaoli Chang
Feiquan Tan
Huai Yang
Xiaoyan Qiu
Liya Luo
Peigao Luo
Potential Role of Photosynthesis in the Regulation of Reactive Oxygen Species and Defence Responses to <i>Blumeria graminis</i> f. sp. <i>tritici</i> in Wheat
International Journal of Molecular Sciences
wheat powdery mildew
photosynthesis
reactive oxygen species
plant defence
salicylic acid
transcriptomics
title Potential Role of Photosynthesis in the Regulation of Reactive Oxygen Species and Defence Responses to <i>Blumeria graminis</i> f. sp. <i>tritici</i> in Wheat
title_full Potential Role of Photosynthesis in the Regulation of Reactive Oxygen Species and Defence Responses to <i>Blumeria graminis</i> f. sp. <i>tritici</i> in Wheat
title_fullStr Potential Role of Photosynthesis in the Regulation of Reactive Oxygen Species and Defence Responses to <i>Blumeria graminis</i> f. sp. <i>tritici</i> in Wheat
title_full_unstemmed Potential Role of Photosynthesis in the Regulation of Reactive Oxygen Species and Defence Responses to <i>Blumeria graminis</i> f. sp. <i>tritici</i> in Wheat
title_short Potential Role of Photosynthesis in the Regulation of Reactive Oxygen Species and Defence Responses to <i>Blumeria graminis</i> f. sp. <i>tritici</i> in Wheat
title_sort potential role of photosynthesis in the regulation of reactive oxygen species and defence responses to i blumeria graminis i f sp i tritici i in wheat
topic wheat powdery mildew
photosynthesis
reactive oxygen species
plant defence
salicylic acid
transcriptomics
url https://www.mdpi.com/1422-0067/21/16/5767
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